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November 20, 2020

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Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei.

This knowledge article accommodates knowledge associated to the analysis article entitled “Copper-mediated on-off management of gene expression in filamentous fungus Trichoderma reesei” (Wang et al., 2017) [1]. 4 sorts of copper responsive promoters had been designed. Quantitative PCR (qPCR) was carried out to find out relative mRNA ranges of purple fluorescent protein gene (rfp) extracted […]