This knowledge article accommodates knowledge associated to the analysis article entitled “Copper-mediated on-off management of gene expression in filamentous fungus Trichoderma reesei” (Wang et al., 2017) [1]. 4 sorts of copper responsive promoters had been designed. Quantitative PCR (qPCR) was carried out to find out relative mRNA ranges of purple fluorescent protein gene (rfp) extracted from cells grown underneath totally different concentrations of CuSO4.
Three deletion vectors had been constructed by utilizing a copper-mediated self-excision cassette as a substitute of a xylose-mediated self-excision cassette (Zhang et al., 2016) [2] to knock out xyn1, one of many two main particular endo-β-1,4-xylanases (Rauscher et al., 2006) [3], xyr1, the important thing transcriptional activator of cellulolytic and xylanolytic genes (Lichius et al., 2015) [4], and ace3, an element important for cellulase manufacturing (Häkkinen et al., 2014) [5]. This knowledge article studies the primers, vector building, and qPCR assay.
Primer Design and Inverse PCR on Yeast Show Antibody Choice Outputs.
The show of antibodies on the floor of Saccharomyces cerevisiae cells permits the high-throughput and exact choice of particular binders for the goal antigen. The current implementation of next-generation sequencing (NGS) to antibody show screening offers a whole image of all the chosen polyclonal inhabitants.
As such, NGS overcomes the constraints of random clones screening, nevertheless it comes with two major limitations: (1) relying upon the platform, the sequencing is normally restricted to the variable heavy chain area complementary figuring out area 3 (HCDR3), or VH gene, and doesn’t present further data on the remainder of the antibody gene, together with the VL; and (2) the sequence-identified clones usually are not bodily out there for validation.
Right here, we describe a fast and efficient protocol based mostly on an inverse-PCR technique to recuperate particular antibody clones based mostly on their HCDR3 sequence from a yeast show choice output.
New design of probe and central-homo primer pairs to enhance TaqMan™ PCR accuracy for HBV detection.
Quantitative PCR (qPCR) assay utilizing TaqMan™ probe was extensively used within the detection of various nucleic acids. Nonetheless, this know-how has a number of drawbacks, together with false destructive outcomes brought on by primer-dimer (PD) and false optimistic points because of primer-probe aggregations.
Right here, we designed a modified TaqMan™-Molecular Beacon probe by including an antisense base and a brand new sort of primer pair named central-homo primer pairs bearing 5-10 bases homologous sequence on the three’ finish. Utilizing the HBV qPCR assay as a proof of idea, the brand new design considerably improved the accuracy of the TaqMan™ qPCR assay for HBV detection.
Software of the central-homo primer pair led to considerably delayed Ct values by 5-10 cycles in contrast with standard primer design. The modified probe containing an antisense base didn’t produce any detectable sign in repeating primer-probe aggregation experiments.
Moreover, using the central-homo primer pair and the non-competitive inner management may resolve the false destructive drawback brought on by PD formation. We validated this personalized duplex qPCR system utilizing 208 medical samples collected from sufferers in clinic exhibiting accuracy was larger than that of the traditional qPCR technique.
Efficient Pure PCR-RFLP Primer Design for SNP Genotyping Utilizing Educating-Studying-Based mostly Optimization With Elite Technique.
SNP (single nucleotide polymorphism) genotyping is the willpower of genetic variations of SNPs between members of a species. In lots of laboratories, PCR-RFLP (polymerase chain reaction-restriction fragment size polymorphism) is a normally used biotechnology for SNP genotyping, particularly in small-scale primary analysis research of complicated genetic ailments.
PCR-RFLP requires an out there restriction enzyme at the least for establish a goal SNP and an efficient primer pair conforms quite a few constraints. Nonetheless, the a number of restriction enzymes, tedious sequence and complex constraints make the mining of obtainable restriction enzymes and the design of efficient primer pairs change into a serious problem.
Within the research, we suggest a novel and out there CI (Computation Intelligence)-based technique known as TLBO (teaching-learning-based optimization) and introduce the elite technique to design efficient primer pairs. Three widespread melting temperature computations can be found within the technique.
REHUNT (Restriction Enzymes HUNTing) is first mixed with the tactic to mine out there restriction enzymes. Sturdy in silico simulations for the GA (genetic algorithm), the PSO (particle swarm optimization), and the tactic for pure PCR-RFLP primer design within the SLC6A4 gene with 200 and eighty-eight SNPs had been carried out and in contrast.
Primers and probe design and precision evaluation of the true time RT-PCR assay in Coxsackievirus A10 and enterovirus detection.
This knowledge article accommodates knowledge associated to the analysis article entitled “Fast detection of enterovirus and Coxsackievirus A10 by a TaqMan based mostly duplex one-step actual time RT-PCR assay” (Chen at al., 2017) [1].
Primers and probe sequence design are among the many most crucial components in real-time polymerase chain response (PCR) assay optimization. Linearity, sensitivity, specificity and precision are the essential standards that are used to judge the efficiency of a brand new technique.
This knowledge article report the primers and probe design and precision evaluation of the brand new assay. VP1 gene of Coxsackievirus A10 (CV-A10) and 5′-NCR of various enterovirus (EV) serotypes had been retrieved from GenBank database and aligned.
Probe qPCR SuperMix |
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20-abx098040 | Abbexa |
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Green qPCR SuperMix |
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abx098031-100l | Abbexa | 100 µl | EUR 675 |
Green qPCR SuperMix |
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abx098031-200l | Abbexa | 200 µl | EUR 912.5 |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056200 | Biochain | 200 reactions | EUR 180 |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056400 | Biochain | 400 reactions | EUR 326 |
Green qPCR SuperMix UDG |
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20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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20-abx098034 | Abbexa |
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Green qPCR SuperMix UDG |
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abx098032-100l | Abbexa | 100 µl | EUR 687.5 |
Green qPCR SuperMix UDG |
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abx098032-200l | Abbexa | 200 µl | EUR 912.5 |
Top Green qPCR SuperMix |
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abx098033-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix |
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abx098033-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix |
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abx098034-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix |
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abx098034-200l | Abbexa | 200 µl | EUR 437.5 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058200 | Biochain | 200 reactions | EUR 211 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058400 | Biochain | 400 reactions | EUR 354 |
Library Quantification qPCR SuperMix |
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20-abx098896 | Abbexa |
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Library Quantification qPCR SuperMix |
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abx098896-100l | Abbexa | 100 µl | EUR 700 |
Library Quantification qPCR SuperMix |
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abx098896-200l | Abbexa | 200 µl | EUR 962.5 |
EnTurbo™ probe qPCR SuperMix |
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EQ017 | ELK Biotech | 5mL | EUR 240 |
Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 | Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
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abx098890-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
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abx098890-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
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abx098891-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
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abx098891-200l | Abbexa | 200 µl | EUR 437.5 |
EnTurbo™ SYBR Color qPCR SuperMix |
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EQ036 | ELK Biotech | 5mL | EUR 340 |
cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems | Abbexa |
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HyperScript RT SuperMix for qPCR |
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K1074-100 | ApexBio | 100 rxn (20 uL/rxn) | EUR 280 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
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K1074-50 | ApexBio | 50 rxn (20 uL/rxn) | EUR 150 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
cDNA Synthesis SuperMix for qPCR |
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abx098019-100l | Abbexa | 100 µl | EUR 825 |
cDNA Synthesis SuperMix for qPCR |
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abx098019-1ml | Abbexa | 1 ml | EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
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abx098019-200l | Abbexa | 200 µl | EUR 1000 |
HiScript II Q RT SuperMix for qPCR |
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R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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EQ035 | ELK Biotech | 5mL | EUR 340 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-100l | Abbexa | 100 µl | EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-200l | Abbexa | 200 µl | Ask for price |
HiScript II Q Select RT SuperMix for qPCR |
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R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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R423-01 | Vazyme | 100rxns(20µl/rxn) | EUR 326 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 217.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-100l | Abbexa | 100 µl | EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-1ml | Abbexa | 1 ml | Ask for price |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-200l | Abbexa | 200 µl | Ask for price |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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EQ033 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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EQ034 | ELK Biotech | 5mL | EUR 340 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
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K1174-100 | ApexBio | 100 rxns | EUR 304 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
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K1174-50 | ApexBio | 50 rxns | EUR 176 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
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R223-01 | Vazyme | 100rxns(20µl/rxn) | EUR 159.5 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054200 | Biochain | 200 reactions | EUR 468 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054400 | Biochain | 400 reactions | EUR 711 |
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
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R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 159.5 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054002 | Biochain | 200 rxn | EUR 468 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054004 | Biochain | 400 rxn | EUR 711 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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R333-01 | Vazyme | 100 rxns (21 μl/rxn) | EUR 217.5 |
PCR SuperMix |
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20-abx098887 | Abbexa |
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2X PCR SuperMix |
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MB200-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
PCR SuperMix (-dye) |
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20-abx098002 | Abbexa |
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PCR SuperMix (+dye) |
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20-abx098003 | Abbexa |
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PCR SuperMix (-dye) |
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abx098002-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (-dye) |
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abx098002-200l | Abbexa | 200 µl | EUR 337.5 |
PCR SuperMix (+dye) |
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abx098003-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (+dye) |
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abx098003-200l | Abbexa | 200 µl | EUR 337.5 |
Plus PCR SuperMix |
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20-abx098888 | Abbexa |
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Evo? cDNA Supermix |
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M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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M1168-25 | Biovision | each | EUR 320.4 |
Plus PCR SuperMix |
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abx098888-100l | Abbexa | 100 µl | EUR 300 |
Plus PCR SuperMix |
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abx098888-200l | Abbexa | 200 µl | EUR 612.5 |
Novo? cDNA Supermix |
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M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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M1169-25 | Biovision | each | EUR 346.8 |
Probe Direct SuperMix |
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abx461057-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe Direct SuperMix |
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abx461057-596tests | Abbexa | 5 × 96 tests | EUR 2800 |
Probe Direct SuperMix |
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abx461057-96tests | Abbexa | 96 tests | EUR 812.5 |
T PCR SuperMix (-dye) |
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20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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20-abx098006 | Abbexa |
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T PCR SuperMix (-dye) |
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abx098005-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (-dye) |
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abx098005-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (+dye) |
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abx098006-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (+dye) |
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abx098006-200l | Abbexa | 200 µl | EUR 462.5 |
cDNA Synthesis SuperMix |
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20-abx09801420ulSystems | Abbexa |
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cDNA Synthesis SuperMix |
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abx098014-100l | Abbexa | 100 µl | EUR 725 |
cDNA Synthesis SuperMix |
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abx098014-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix |
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abx098014-200l | Abbexa | 200 µl | EUR 812.5 |
AmpHS 2X PCR SuperMix |
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MB203-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055200 | Biochain | 200 reactions | EUR 427 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055400 | Biochain | 400 reactions | EUR 682 |
EasyPfu PCR SuperMix (-dye) |
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20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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20-abx098010 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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abx098010-100l | Abbexa | 100 µl | EUR 300 |
FastPfu PCR SuperMix (-dye) |
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abx098010-200l | Abbexa | 200 µl | EUR 575 |
Library Amplification SuperMix |
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20-abx098894 | Abbexa |
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Library Amplification SuperMix |
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abx098894-100l | Abbexa | 100 µl | EUR 737.5 |
Library Amplification SuperMix |
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abx098894-200l | Abbexa | 200 µl | EUR 1137.5 |
OneScriptcDNA Synthesis SuperMix |
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G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
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20-abx098004 | Abbexa |
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PCR SuperMix for PAGE (+dye) |
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abx098004-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
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abx098004-200l | Abbexa | 200 µl | EUR 337.5 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055002 | Biochain | 200 rxn | EUR 426 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055004 | Biochain | 400 rxn | EUR 682 |
OneScriptPlus cDNA Synthesis SuperMix |
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G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
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MBA01-0100 | Bio-Helix | 100 rxns (2.5ml) | EUR 16 |
EnTurbo™ SYBR Green PCR SuperMix |
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EQ001 | ELK Biotech | 5mL | EUR 340 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
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MB204-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 13 |
Green Two-Step qRT-PCR SuperMix |
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abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 777.6 |
Green One-Step qRT-PCR SuperMix |
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20-abx09803820ulSystems | Abbexa |
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Probe One-Step qRT-PCR SuperMix |
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20-abx09804220ulSystems | Abbexa |
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Green Two-Step qRT-PCR SuperMix |
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abx098035-100l | Abbexa | 100 µl | EUR 925 |
Green Two-Step qRT-PCR SuperMix |
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abx098035-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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abx098035-200l | Abbexa | 200 µl | Ask for price |
Green One-Step qRT-PCR SuperMix |
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abx098038-100l | Abbexa | 100 µl | EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
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abx098038-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix |
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abx098038-200l | Abbexa | 200 µl | EUR 1050 |
Probe One-Step qRT-PCR SuperMix |
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abx098042-100l | Abbexa | 100 µl | EUR 850 |
Probe One-Step qRT-PCR SuperMix |
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abx098042-1ml | Abbexa | 1 ml | Ask for price |
Probe One-Step qRT-PCR SuperMix |
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abx098042-200l | Abbexa | 200 µl | EUR 1400 |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 727.2 |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 910.8 |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-100l | Abbexa | 100 µl | EUR 887.5 |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-200l | Abbexa | 200 µl | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-100l | Abbexa | 100 µl | EUR 1087.5 |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-200l | Abbexa | 200 µl | Ask for price |
AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X) |
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MB202-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
All-in-One cDNA Synthesis SuperMix |
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B24403 | Bimake | 200 ractions | EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
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B24408 | Bimake | 1000 ractions | EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
OmniPCR HS Supermix w Fluorescent dye |
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MBA03-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OmniPCR FAST Supermix w Fluorescent dye |
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MBA02-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OmniPCR Long Supermix w Fluorescent dye |
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MBA04-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 727.2 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 794.4 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 1028.4 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-100l | Abbexa | 100 µl | EUR 875 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-200l | Abbexa | 200 µl | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-100l | Abbexa | 100 µl | EUR 950 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-200l | Abbexa | 200 µl | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-100l | Abbexa | 100 µl | EUR 1212.5 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-200l | Abbexa | 200 µl | Ask for price |
Fly First-Strand cDNA Synthesis SuperMix |
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20-abx09885820ulSystems | Abbexa |
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Fly First-Strand cDNA Synthesis SuperMix |
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abx098858-100l | Abbexa | 100 µl | EUR 850 |
Fly First-Strand cDNA Synthesis SuperMix |
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abx098858-1ml | Abbexa | 1 ml | Ask for price |
Fly First-Strand cDNA Synthesis SuperMix |
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abx098858-200l | Abbexa | 200 µl | EUR 1037.5 |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems | Abbexa | 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) | EUR 1062 |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-100l | Abbexa | 100 µl | EUR 1212.5 |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-1ml | Abbexa | 1 ml | Ask for price |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-200l | Abbexa | 200 µl | Ask for price |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-20rxns20ulSystems | Abbexa | 20 rxns × 20 ul Systems | EUR 878.4 |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-100l | Abbexa | 100 µl | EUR 950 |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-1ml | Abbexa | 1 ml | Ask for price |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-200l | Abbexa | 200 µl | Ask for price |
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
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20-abx098007 | Abbexa |
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Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 961.2 |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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20-abx09803920ulSystems | Abbexa |
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Probe One-Step qRT-PCR SuperMix (GC Rich) |
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20-abx09804120ulSystems | Abbexa |
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High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
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abx098007-100l | Abbexa | 100 µl | EUR 262.5 |
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
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abx098007-200l | Abbexa | 200 µl | EUR 475 |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-100l | Abbexa | 100 µl | EUR 1125 |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-200l | Abbexa | 200 µl | Ask for price |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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abx098039-100l | Abbexa | 100 µl | EUR 450 |
The intra- and inter-assay variation had been assessed utilizing excessive, medium and low focus of management plasmid DNA and viral RNA samples.