Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei.

Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei.

This knowledge article accommodates knowledge associated to the analysis article entitled “Copper-mediated on-off management of gene expression in filamentous fungus Trichoderma reesei” (Wang et al., 2017) [1]. 4 sorts of copper responsive promoters had been designed. Quantitative PCR (qPCR) was carried out to find out relative mRNA ranges of purple fluorescent protein gene (rfp) extracted from cells grown underneath totally different concentrations of CuSO4.

Three deletion vectors had been constructed by utilizing a copper-mediated self-excision cassette as a substitute of a xylose-mediated self-excision cassette (Zhang et al., 2016) [2] to knock out xyn1, one of many two main particular endo-β-1,4-xylanases (Rauscher et al., 2006) [3], xyr1, the important thing transcriptional activator of cellulolytic and xylanolytic genes (Lichius et al., 2015) [4], and ace3, an element important for cellulase manufacturing (Häkkinen et al., 2014) [5]. This knowledge article studies the primers, vector building, and qPCR assay.

Primer Design and Inverse PCR on Yeast Show Antibody Choice Outputs.

The show of antibodies on the floor of Saccharomyces cerevisiae cells permits the high-throughput and exact choice of particular binders for the goal antigen. The current implementation of next-generation sequencing (NGS) to antibody show screening offers a whole image of all the chosen polyclonal inhabitants.

As such, NGS overcomes the constraints of random clones screening, nevertheless it comes with two major limitations: (1) relying upon the platform, the sequencing is normally restricted to the variable heavy chain area complementary figuring out area 3 (HCDR3), or VH gene, and doesn’t present further data on the remainder of the antibody gene, together with the VL; and (2) the sequence-identified clones usually are not bodily out there for validation.

Right here, we describe a fast and efficient protocol based mostly on an inverse-PCR technique to recuperate particular antibody clones based mostly on their HCDR3 sequence from a yeast show choice output.

New design of probe and central-homo primer pairs to enhance TaqMan™ PCR accuracy for HBV detection.

Quantitative PCR (qPCR) assay utilizing TaqMan™ probe was extensively used within the detection of various nucleic acids. Nonetheless, this know-how has a number of drawbacks, together with false destructive outcomes brought on by primer-dimer (PD) and false optimistic points because of primer-probe aggregations.

Right here, we designed a modified TaqMan™-Molecular Beacon probe by including an antisense base and a brand new sort of primer pair named central-homo primer pairs bearing 5-10 bases homologous sequence on the three’ finish. Utilizing the HBV qPCR assay as a proof of idea, the brand new design considerably improved the accuracy of the TaqMan™ qPCR assay for HBV detection.

Software of the central-homo primer pair led to considerably delayed Ct values by 5-10 cycles in contrast with standard primer design. The modified probe containing an antisense base didn’t produce any detectable sign in repeating primer-probe aggregation experiments.

Moreover, using the central-homo primer pair and the non-competitive inner management may resolve the false destructive drawback brought on by PD formation. We validated this personalized duplex qPCR system utilizing 208 medical samples collected from sufferers in clinic exhibiting accuracy was larger than that of the traditional qPCR technique.

Efficient Pure PCR-RFLP Primer Design for SNP Genotyping Utilizing Educating-Studying-Based mostly Optimization With Elite Technique.

SNP (single nucleotide polymorphism) genotyping is the willpower of genetic variations of SNPs between members of a species. In lots of laboratories, PCR-RFLP (polymerase chain reaction-restriction fragment size polymorphism) is a normally used biotechnology for SNP genotyping, particularly in small-scale primary analysis research of complicated genetic ailments.

PCR-RFLP requires an out there restriction enzyme at the least for establish a goal SNP and an efficient primer pair conforms quite a few constraints. Nonetheless, the a number of restriction enzymes, tedious sequence and complex constraints make the mining of obtainable restriction enzymes and the design of efficient primer pairs change into a serious problem.

 Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei.

Within the research, we suggest a novel and out there CI (Computation Intelligence)-based technique known as TLBO (teaching-learning-based optimization) and introduce the elite technique to design efficient primer pairs. Three widespread melting temperature computations can be found within the technique.

REHUNT (Restriction Enzymes HUNTing) is first mixed with the tactic to mine out there restriction enzymes. Sturdy in silico simulations for the GA (genetic algorithm), the PSO (particle swarm optimization), and the tactic for pure PCR-RFLP primer design within the SLC6A4 gene with 200 and eighty-eight SNPs had been carried out and in contrast.

Primers and probe design and precision evaluation of the true time RT-PCR assay in Coxsackievirus A10 and enterovirus detection.

This knowledge article accommodates knowledge associated to the analysis article entitled “Fast detection of enterovirus and Coxsackievirus A10 by a TaqMan based mostly duplex one-step actual time RT-PCR assay” (Chen at al., 2017) [1].

Primers and probe sequence design are among the many most crucial components in real-time polymerase chain response (PCR) assay optimization. Linearity, sensitivity, specificity and precision are the essential standards that are used to judge the efficiency of a brand new technique.

This knowledge article report the primers and probe design and precision evaluation of the brand new assay. VP1 gene of Coxsackievirus A10 (CV-A10) and 5′-NCR of various enterovirus (EV) serotypes had been retrieved from GenBank database and aligned.

Pro QPCR SuperMix Kit

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cDNA Synthesis SuperMix for qPCR

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Top Green qPCR SuperMix (+Dye II)

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Tip Green qPCR SuperMix (+Dye II)

20-abx098891
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abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
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HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
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R232-01 100 rxn (20 μl/rxn)
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HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
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PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
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PCR SuperMix

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CyanRed Power Box

2305226 2unit
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Description: i ndepent l y use for CyanRed Epi

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106 500 g
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Power Broth™

117 500 ml
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electrophoresis power supply

EV245 ea
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20-abx098002
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PCR SuperMix (+dye)

20-abx098003
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cDNA Synthesis SuperMix

20-abx09801420ulSystems
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Plus PCR SuperMix

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Library Amplification SuperMix

20-abx098894
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OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 141

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

qPCR Control Template Kit

G929 100 rxns
EUR 148

QPCR Positive Control Kit

M1126-100
EUR 403

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns Ask for price

COLLECTION CONTAINER 1L, EVA FILM

91-200-01 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

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91-200-02 1/pk
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COLLECTION CONTAINER 1L, EVA FILM

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COLLECTION CONTAINER 5L, EVA FILM

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COLLECTION CONTAINER 10L, EVA FILM

91-200-45 1/pk
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COLLECTION CONTAINER 20L, EVA FILM

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COLLECTION CONTAINER 50L, EVA FILM

91-200-48 1/pk
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COLLECTION CONTAINER 100L, EVA FILM

91-200-82 1/pk
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Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 200L, EVA FILM

91-200-83 1/pk
EUR 292
Description: Flexible Containers; Bioprocess Bags and Parts

Power Prime Broth™

112 500 g
EUR 111

Power Prime-olate™

162 500 g
EUR 106

Power Prime-olate™

172 500 ml
EUR 68

Power Prime Broth™

122 500 ml
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Alkaline phosphatase Power AP

AZ140A 1MG
EUR 100.46
  • Product category: Proteins/Enzymes/Phosphatases

Alkaline phosphatase Power AP

AZ235A 1MG
EUR 116.12
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Four Source Power Supply 

EDK621 1 UNIT
EUR 684.23
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12v vehicle power adapter

BSH100-A12 1 PC
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T PCR SuperMix (-dye)

20-abx098005
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T PCR SuperMix (+dye)

20-abx098006
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  • EUR 342.00
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EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 203.00
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FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 244.00
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OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
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Built in power supply for WSE-1165 Power AC 100-240V

WSE-650-659 1unit
EUR 726
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n

Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

COLLECTION CONTAINER 500 ML, EVA FILM

91-200-41 1/pk
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Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Power leads for HorizeBLOT-R

2322462 5unit
EUR 270

Dual Source 150 Power Supply 

EDK620 1 UNIT
EUR 371.46
  • Product category: Benchtop Equipment/Gel Electrophoresis/Power Supplies

electrophoresis power supply uk plug

EV245-UK ea
EUR 479

electrophoresis power supply 120 vac

EV245-US ea
EUR 479

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
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Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

igScript RT-qPCR Kit - 50 Reactions

4513 1/EA
EUR 248

igScript RT-qPCR Kit - 200 Reactions

4515 1/EA
EUR 716

Coronavirus Rapid RT-qPCR Detection Kit

K1461-100 100 Rxns
EUR 551

Adeno-Associated Virus qPCR Quantification Kit

K1473-100 100 Rxns
EUR 348

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

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Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

K515-200
EUR 550

OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit

STA-859 200 assays
EUR 508
Description: OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples. Following the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the sample, the kit colorimetric probe develops a blue color that is read colorimetrically at 540-600nm. The antioxidant potential of samples is determined based on an iron standard curve and results are calculated at Fe2+ equivalents (µM) or FRAP value.

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110
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Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999
  • Category: Lineage Profilers

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172
  • Category: MicroRNA Tools

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 880
  • Category: Long Non-coding RNA Tools

Protein Eva-1 Homolog C (EVA1C) Antibody

20-abx302696
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
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QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 376
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 572
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

myVolt™ Mini power supply, 115V

BM0294 Ea
EUR 307

myVolt™ Mini power supply, 230V

BM0295 Ea
EUR 307

myVolt™ Touch power supply, 115V

BM0296 Ea
EUR 718

myVolt™ Touch power supply, 230V

BM0297 Ea
EUR 718

ENDURO™ 250V Power Supply , 120V

LE0203-120 Ea
EUR 1603

ENDURO™ 250V Power Supply , 230V

LE0203-230 Ea
EUR 1603

ENDURO™ 300V Power Supply , 120V

LE0303-120 Ea
EUR 1198

myVolt™ Mini Power Supply,115V

LE1007 Ea
EUR 307

myVolt™ Mini Power Supply, 230V

LE1008 Ea
EUR 307

myVolt™ Touch power supply, 115V

LE1009 Ea
EUR 718

myVolt™ Touch power supply, 220V

LE1010 Ea
EUR 718

UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET

MOTO-SP-PWR 1/pk
EUR 61
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 732
  • Shipped within 5-10 working days.

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 648
  • Shipped within 5-10 working days.

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
  • EUR 495.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
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  • Shipped within 5-10 working days.

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
  • EUR 592.00
  • EUR 1024.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • EUR 718.00
  • EUR 578.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 10 x 1,25 ml
EUR 1719

COVID-19 RT-qPCR Rapid Detection Kit

G628 NULL
EUR 0

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 20 reactions
EUR 331
  • Category: PiggyBac Technology

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

FRAP? (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate)

K043-H1 2 x 96 well plate
EUR 496

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-iCycler

MasterMix-5PC 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex

MasterMix-5PM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

The intra- and inter-assay variation had been assessed utilizing excessive, medium and low focus of management plasmid DNA and viral RNA samples.

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