This knowledge article accommodates knowledge associated to the analysis article entitled “Copper-mediated on-off management of gene expression in filamentous fungus Trichoderma reesei” (Wang et al., 2017) [1]. 4 sorts of copper responsive promoters had been designed. Quantitative PCR (qPCR) was carried out to find out relative mRNA ranges of purple fluorescent protein gene (rfp) extracted from cells grown underneath totally different concentrations of CuSO4.
Three deletion vectors had been constructed by utilizing a copper-mediated self-excision cassette as a substitute of a xylose-mediated self-excision cassette (Zhang et al., 2016) [2] to knock out xyn1, one of many two main particular endo-β-1,4-xylanases (Rauscher et al., 2006) [3], xyr1, the important thing transcriptional activator of cellulolytic and xylanolytic genes (Lichius et al., 2015) [4], and ace3, an element important for cellulase manufacturing (Häkkinen et al., 2014) [5]. This knowledge article studies the primers, vector building, and qPCR assay.
Primer Design and Inverse PCR on Yeast Show Antibody Choice Outputs.
The show of antibodies on the floor of Saccharomyces cerevisiae cells permits the high-throughput and exact choice of particular binders for the goal antigen. The current implementation of next-generation sequencing (NGS) to antibody show screening offers a whole image of all the chosen polyclonal inhabitants.
As such, NGS overcomes the constraints of random clones screening, nevertheless it comes with two major limitations: (1) relying upon the platform, the sequencing is normally restricted to the variable heavy chain area complementary figuring out area 3 (HCDR3), or VH gene, and doesn’t present further data on the remainder of the antibody gene, together with the VL; and (2) the sequence-identified clones usually are not bodily out there for validation.
Right here, we describe a fast and efficient protocol based mostly on an inverse-PCR technique to recuperate particular antibody clones based mostly on their HCDR3 sequence from a yeast show choice output.
New design of probe and central-homo primer pairs to enhance TaqMan™ PCR accuracy for HBV detection.
Quantitative PCR (qPCR) assay utilizing TaqMan™ probe was extensively used within the detection of various nucleic acids. Nonetheless, this know-how has a number of drawbacks, together with false destructive outcomes brought on by primer-dimer (PD) and false optimistic points because of primer-probe aggregations.
Right here, we designed a modified TaqMan™-Molecular Beacon probe by including an antisense base and a brand new sort of primer pair named central-homo primer pairs bearing 5-10 bases homologous sequence on the three’ finish. Utilizing the HBV qPCR assay as a proof of idea, the brand new design considerably improved the accuracy of the TaqMan™ qPCR assay for HBV detection.
Software of the central-homo primer pair led to considerably delayed Ct values by 5-10 cycles in contrast with standard primer design. The modified probe containing an antisense base didn’t produce any detectable sign in repeating primer-probe aggregation experiments.
Moreover, using the central-homo primer pair and the non-competitive inner management may resolve the false destructive drawback brought on by PD formation. We validated this personalized duplex qPCR system utilizing 208 medical samples collected from sufferers in clinic exhibiting accuracy was larger than that of the traditional qPCR technique.
Efficient Pure PCR-RFLP Primer Design for SNP Genotyping Utilizing Educating-Studying-Based mostly Optimization With Elite Technique.
SNP (single nucleotide polymorphism) genotyping is the willpower of genetic variations of SNPs between members of a species. In lots of laboratories, PCR-RFLP (polymerase chain reaction-restriction fragment size polymorphism) is a normally used biotechnology for SNP genotyping, particularly in small-scale primary analysis research of complicated genetic ailments.
PCR-RFLP requires an out there restriction enzyme at the least for establish a goal SNP and an efficient primer pair conforms quite a few constraints. Nonetheless, the a number of restriction enzymes, tedious sequence and complex constraints make the mining of obtainable restriction enzymes and the design of efficient primer pairs change into a serious problem.
Within the research, we suggest a novel and out there CI (Computation Intelligence)-based technique known as TLBO (teaching-learning-based optimization) and introduce the elite technique to design efficient primer pairs. Three widespread melting temperature computations can be found within the technique.
REHUNT (Restriction Enzymes HUNTing) is first mixed with the tactic to mine out there restriction enzymes. Sturdy in silico simulations for the GA (genetic algorithm), the PSO (particle swarm optimization), and the tactic for pure PCR-RFLP primer design within the SLC6A4 gene with 200 and eighty-eight SNPs had been carried out and in contrast.
Primers and probe design and precision evaluation of the true time RT-PCR assay in Coxsackievirus A10 and enterovirus detection.
This knowledge article accommodates knowledge associated to the analysis article entitled “Fast detection of enterovirus and Coxsackievirus A10 by a TaqMan based mostly duplex one-step actual time RT-PCR assay” (Chen at al., 2017) [1].
Primers and probe sequence design are among the many most crucial components in real-time polymerase chain response (PCR) assay optimization. Linearity, sensitivity, specificity and precision are the essential standards that are used to judge the efficiency of a brand new technique.
This knowledge article report the primers and probe design and precision evaluation of the brand new assay. VP1 gene of Coxsackievirus A10 (CV-A10) and 5′-NCR of various enterovirus (EV) serotypes had been retrieved from GenBank database and aligned.
Probe qPCR SuperMix |
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20-abx098040 | Abbexa |
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Library Quantification qPCR SuperMix |
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20-abx098896 | Abbexa |
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Green qPCR SuperMix UDG |
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20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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20-abx098034 | Abbexa |
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QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054200 | Biochain | 200 reactions | EUR 489.6 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054400 | Biochain | 400 reactions | EUR 712.8 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054004 | Biochain | 400 rxn | EUR 635 |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056200 | Biochain | 200 reactions | EUR 225.6 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056400 | Biochain | 400 reactions | EUR 358.8 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems | Abbexa |
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Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054002 | Biochain | 200 rxn | EUR 418 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058200 | Biochain | 200 reactions | EUR 253.2 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058400 | Biochain | 400 reactions | EUR 384 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 | Abbexa |
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cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
HiScript II Q RT SuperMix for qPCR |
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R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 282 |
HiScript II Q Select RT SuperMix for qPCR |
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R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 282 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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HS-100-QPCR | CORNING | 100/pk | EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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HS-150-QPCR | CORNING | 100/pk | EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 351.6 |
PCR SuperMix |
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20-abx098887 | Abbexa |
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HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 292.8 |
Plus PCR SuperMix |
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20-abx098888 | Abbexa |
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Library Amplification SuperMix |
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20-abx098894 | Abbexa |
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PCR SuperMix (-dye) |
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20-abx098002 | Abbexa |
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PCR SuperMix (+dye) |
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20-abx098003 | Abbexa |
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cDNA Synthesis SuperMix |
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20-abx09801420ulSystems | Abbexa |
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OneScriptcDNA Synthesis SuperMix |
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G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
Evo? cDNA Supermix |
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M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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M1168-25 | Biovision | each | EUR 320.4 |
Novo? cDNA Supermix |
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M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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M1169-25 | Biovision | each | EUR 346.8 |
QPCR Kit QPCR Mastermix DNA |
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MOL6354 | Scientific Laboratory Supplies | EACH | EUR 231.42 |
electrophoresis power supply |
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EV245 | Consort | ea | EUR 574.8 |
CyanRed Power Box |
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2305226 | Atto | 2unit | EUR 339.6 |
Description: i ndepent l y use for CyanRed Epi |
Replacement power adapter |
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HOM2748 | Scientific Laboratory Supplies | EACH | EUR 271.32 |
Electrode InLab Power |
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51343110 | Scientific Laboratory Supplies | EACH | EUR 405.84 |
T PCR SuperMix (-dye) |
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20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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20-abx098006 | Abbexa |
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EasyPfu PCR SuperMix (-dye) |
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20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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20-abx098010 | Abbexa |
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OneScriptPlus cDNA Synthesis SuperMix |
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G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
COLLECTION CONTAINER 1L, EVA FILM |
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91-200-01 | CORNING | 1/pk | EUR 96 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 2L, EVA FILM |
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91-200-02 | CORNING | 1/pk | EUR 105.6 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
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91-200-05 | CORNING | 1/pk | EUR 118.8 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
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91-200-10 | CORNING | 1/pk | EUR 121.2 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
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91-200-20 | CORNING | 1/pk | EUR 132 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
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91-200-36 | CORNING | 1/pk | EUR 103.2 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
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91-200-39 | CORNING | 1/pk | EUR 106.8 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
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91-200-43 | CORNING | 1/pk | EUR 181.2 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
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91-200-45 | CORNING | 1/pk | EUR 151.2 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
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91-200-47 | CORNING | 1/pk | EUR 158.4 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 50L, EVA FILM |
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91-200-48 | CORNING | 1/pk | EUR 175.2 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 100L, EVA FILM |
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91-200-82 | CORNING | 1/pk | EUR 334.8 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 200L, EVA FILM |
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91-200-83 | CORNING | 1/pk | EUR 350.4 |
Description: Flexible Containers; Bioprocess Bags and Parts |
Collection Container 1L EVA Film |
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91-200-42 | Scientific Laboratory Supplies | EACH | EUR 54.38 |
12v vehicle power adapter |
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BSH100-A12 | Benchmark Scientific | 1 PC | EUR 515.44 |
Alkaline phosphatase Power AP |
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AZ140A | Bio Basic | 1MG | EUR 120.55 |
Alkaline phosphatase Power AP |
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AZ235A | Bio Basic | 1MG | EUR 139.34 |
Four Source Power Supply |
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EDK621 | Bio Basic | 1 UNIT | EUR 821.08 |
Consort Power Supply EV2230 |
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ELE9104 | Scientific Laboratory Supplies | EACH | EUR 1185.6 |
Consort Power Supply EV2310 |
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ELE9106 | Scientific Laboratory Supplies | EACH | EUR 1077.3 |
Consort Power Supply EV2320 |
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ELE9108 | Scientific Laboratory Supplies | EACH | EUR 1185.6 |
Consort Power Supply EV2650 |
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ELE9110 | Scientific Laboratory Supplies | EACH | EUR 1077.3 |
Consort Power Supply EV3020 |
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ELE9112 | Scientific Laboratory Supplies | EACH | EUR 1553.82 |
Consort Power Supply EV3150 |
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ELE9114 | Scientific Laboratory Supplies | EACH | EUR 1553.82 |
Consort Power Supply EV3330 |
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ELE9116 | Scientific Laboratory Supplies | EACH | EUR 2330.16 |
Consort Power Supply EV3610 |
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ELE9118 | Scientific Laboratory Supplies | EACH | EUR 1553.82 |
Consort Power Supply EV3620 |
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ELE9120 | Scientific Laboratory Supplies | EACH | EUR 2330.16 |
Mains Power Adaptor Cord |
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46001724 | Scientific Laboratory Supplies | EACH | EUR 28.5 |
Power charger 220 V |
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DD75014 | Scientific Laboratory Supplies | EACH | EUR 134.52 |
12v vehicle power adapter |
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BLO1258 | Scientific Laboratory Supplies | EACH | EUR 66.12 |
Eps 301 Power Supply |
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18113001 | Scientific Laboratory Supplies | EACH | EUR 762.66 |
Frit Kit |
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FRIT-KIT | Next Advance | 1each | EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Column Packing Kit |
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PACK-KIT | Next Advance | 1pack | EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
Built in power supply for WSE-1165 Power AC 100-240V |
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WSE-650-659 | Atto | 1unit | EUR 871.2 |
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n |
COLLECTION CONTAINER 500 ML, EVA FILM |
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91-200-41 | CORNING | 1/pk | EUR 82.8 |
Description: Flexible Containers; Bioprocess Bags and Parts |
PCR SuperMix for PAGE (+dye) |
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20-abx098004 | Abbexa |
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PCR Mycoplasma Detection Kit |
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M034-Kit | TOKU-E | Kit | EUR 319.2 |
qPCR Control Template Kit |
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G929 | ABM | 100 rxns | EUR 177.6 |
Lentivirus qPCR Quantification Kit |
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K1471-100 | Biovision | 100 Rxns | EUR 357.6 |
Retrovirus qPCR Quantification Kit |
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K1472-100 | Biovision | 100 Rxns | EUR 381.6 |
Entrans qPCR Probe Kit |
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RK21209 | Abclonal | 40 RXN | Ask for price |
QPCR Kit DNA Staphylococ |
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MOL6370 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Bifidobacte |
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MOL6418 | Scientific Laboratory Supplies | EACH | EUR 842.46 |
QPCR Kit DNA Clostridium |
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MOL6422 | Scientific Laboratory Supplies | EACH | EUR 876.66 |
QPCR Kit DNA Lactobacill |
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MOL6474 | Scientific Laboratory Supplies | EACH | EUR 876.66 |
QPCR Kit DNA Anacardium |
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MOL6486 | Scientific Laboratory Supplies | EACH | EUR 842.46 |
QPCR Kit DNA Bertholleti |
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MOL6498 | Scientific Laboratory Supplies | EACH | EUR 876.66 |
QPCR Kit DNA Melanogramm |
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MOL6550 | Scientific Laboratory Supplies | EACH | EUR 876.66 |
QPCR Kit DNA Merlangius |
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MOL6562 | Scientific Laboratory Supplies | EACH | EUR 842.46 |
QPCR Kit DNA Phacochoeru |
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MOL6574 | Scientific Laboratory Supplies | EACH | EUR 876.66 |
QPCR Kit DNA Pleuronecte |
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MOL6582 | Scientific Laboratory Supplies | EACH | EUR 842.46 |
QPCR Kit DNA Pollachius |
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MOL6594 | Scientific Laboratory Supplies | EACH | EUR 842.46 |
QPCR Kit DNA Bifidobacte |
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MOL6614 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Clostridium |
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MOL6630 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Enterococcu |
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MOL6634 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Enterococcu |
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MOL6638 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Pseudomonas |
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MOL6666 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Cyclospora |
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MOL6694 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Campylobact |
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MOL6702 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Escherichia |
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MOL6710 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Escherichia |
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MOL6718 | Scientific Laboratory Supplies | EACH | EUR 1081.86 |
QPCR Kit DNA Enteropatho |
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MOL6734 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Francisella |
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MOL6738 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycobacteri |
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MOL6750 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Shewanella |
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MOL6758 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Aggregatiba |
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MOL6762 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Acinetobact |
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MOL6766 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Ajellomyces |
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MOL6770 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Balamuthia |
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MOL6806 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Bordetella |
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MOL6818 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Clostridium |
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MOL6826 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Clostridium |
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MOL6830 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Clostridium |
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MOL6838 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Cytomegalov |
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MOL6854 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Enterococcu |
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MOL6866 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Haemophilus |
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MOL6918 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit RNA Coronavirus |
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MOL6946 | Scientific Laboratory Supplies | EACH | EUR 820.8 |
QPCR Kit DNA Klebsiella |
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MOL8156 | Scientific Laboratory Supplies | EACH | EUR 1220.94 |
QPCR Kit DNA Leishmania |
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MOL8164 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Legionella |
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MOL8168 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Legionella |
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MOL8176 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycobacteri |
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MOL8192 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Mycoplasma |
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MOL8204 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycobacteri |
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MOL8212 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycoplasma |
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MOL8216 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycobacteri |
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MOL8220 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Methicillin |
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MOL8228 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Methicillin |
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MOL8232 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Methicillin |
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MOL8234 | Scientific Laboratory Supplies | EACH | EUR 982.68 |
QPCR Kit DNA Methicillin |
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MOL8236 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Methicillin |
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MOL8238 | Scientific Laboratory Supplies | EACH | EUR 982.68 |
QPCR Kit DNA Oxalobacter |
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MOL8252 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Plasmodium |
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MOL8256 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Porphyromon |
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MOL8260 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Prevotella |
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MOL8264 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Pneumocysti |
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MOL8268 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Rickettsia |
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MOL8296 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit RNA Respiratory |
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MOL8300 | Scientific Laboratory Supplies | EACH | EUR 1220.94 |
QPCR Kit RNA Respiratory |
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MOL8304 | Scientific Laboratory Supplies | EACH | EUR 1220.94 |
QPCR Kit RNA Respiratory |
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MOL8308 | Scientific Laboratory Supplies | EACH | EUR 1220.94 |
QPCR Kit DNA Staphylococ |
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MOL8316 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Staphylococ |
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MOL8320 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Streptococc |
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MOL8328 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Tannerella |
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MOL8356 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Tsukamurell |
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MOL8360 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Trichomonas |
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MOL8368 | Scientific Laboratory Supplies | EACH | EUR 896.04 |
QPCR Kit DNA Mycobacteri |
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MOL8372 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Trichodyspl |
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MOL8380 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Ureaplasma |
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MOL8384 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Blastocysti |
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MOL8400 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Ancylostoma |
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MOL8404 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Aspergillus |
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MOL8412 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Aspergillus |
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MOL8424 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA AspergillusMmix |
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MOL8426 | Scientific Laboratory Supplies | EACH | EUR 1069.32 |
QPCR Kit DNA Burkholderi |
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MOL8432 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Chlamydophi |
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MOL8436 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Campylobact |
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MOL8444 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Chlamydiace |
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MOL8448 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Cryptococcu |
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MOL8452 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Chlamydophi |
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MOL8456 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
The intra- and inter-assay variation had been assessed utilizing excessive, medium and low focus of management plasmid DNA and viral RNA samples.