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A Novel Teaching-Learning-Based Optimization for Improved Mutagenic Primer Design in Mismatch PCR-RFLP SNP Genotyping.

A Novel Teaching-Learning-Based Optimization for Improved Mutagenic Primer Design in Mismatch PCR-RFLP SNP Genotyping.

Many single nucleotide polymorphisms (SNPs) for advanced genetic ailments are genotyped by polymerase chain reaction-restriction fragment size polymorphism (PCR-RFLP) in small-scale fundamental analysis research. It’s a vital work to design possible PCR-RFLP primer pair and discover out accessible restriction enzymes to acknowledge the goal SNP for PCR experiments. Nevertheless, many SNPs are incapable of performing […]

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PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.

PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.

We just lately demonstrated that the Escherichia coli ribosome is strong sufficient to accommodate international 16S rRNAs from numerous gamma- and betaproteobacteria micro organism (Kitahara et al., 2012). Therein, we used the widespread common primers Bac8f and UN1541r to acquire a virtually full-length gene. Nevertheless, we observed that these primers overlap variable websites at 19[A/C] […]

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