Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays.

Analytical PCR experiments ideally use inside probes for monitoring the amplification response and particular detection of the amplicon. Such inside probes must be designed in shut context with the amplification primers, and will require further issues for the detection of genetic variations.

Right here we describe Edesign, a brand new on-line and stand-alone software for designing units of PCR primers along with an inside probe for conducting quantitative real-time PCR (qPCR) and genotypic experiments. Edesign can be utilized for choosing commonplace DNA oligonucleotides like as an example TaqMan probes, however has been additional prolonged with new features and enhanced design options for Eprobes.

Eprobes, with their single thiazole orange-labelled nucleotide, permit for extremely delicate genotypic assays due to their increased DNA binding affinity as in comparison with commonplace DNA oligonucleotides. Utilizing new thermodynamic parameters, Edesign considers distinctive options of Eprobes throughout primer and probe design for establishing qPCR experiments and genotyping by melting curve evaluation.

Design and analysis of reverse transcription nested PCR primers for the detection of betanodavirus in finfish.

Viral encephalopathy and retinopathy in any other case generally known as viral nervous necrosis (VNN) is a neuropathological situation affecting greater than 50 fish species worldwide, largely marine.

Completely different PCR protocols with particular primers had been reported from many international locations for affirmation of VNN in fishes. Within the current examine, two pairs of primers had been designed and evaluated for the prognosis of medical and subclinical circumstances of infections from subject.

These primers designated as BARL-F1/BARL-R1 amplified a 902 bp product within the variable area (T4) of the coat protein gene by first step PCR. Nested PCR primers BARL-F2/BARL-R2 amplified a fraction of 313 bp. The outcomes had been comparable with different generally used primer units similar to F2/R3 and RG668f/RG919r primers.

These new primers might detect betanodavirus in commonplace reference samples containing low, reasonable and excessive viral load. Identified constructive and unfavourable management samples of fish additionally revealed a predictive worth of 100 % by RT-PCR prognosis.

MRPrimer: a MapReduce-based technique for the thorough design of legitimate and ranked primers for PCR.

Primer design is a elementary method that’s extensively used for polymerase chain response (PCR). Though many strategies have been proposed for primer design, they require quite a lot of handbook effort to generate possible and legitimate primers, together with homology exams on off-target sequences utilizing BLAST-like instruments.

That strategy is inconvenient for a lot of goal sequences of quantitative PCR (qPCR) attributable to contemplating the identical stringent and allele-invariant constraints. To deal with this problem, we suggest a completely new technique referred to as MRPrimer that may design all possible and legitimate primer pairs present in a DNA database directly, whereas concurrently checking a mess of filtering constraints and validating primer specificity.

Moreover, MRPrimer suggests the very best primer pair for every goal sequence, primarily based on a rating technique. By way of qPCR evaluation utilizing 343 primer pairs and the corresponding sequencing and comparative analyses, we confirmed that the primer pairs designed by MRPrimer are very steady and efficient for qPCR.

As well as, MRPrimer is computationally environment friendly and scalable and due to this fact helpful for rapidly developing a complete assortment of possible and legitimate primers for steadily up to date databases like RefSeq. Moreover, we propose that MRPrimer could be utilized conveniently for experiments requiring primer design, particularly real-time qPCR.

STITCHER: An internet useful resource for high-throughput design of primers for overlapping PCR purposes.

Overlapping PCR is routinely utilized in a large variety of molecular purposes. These embody stitching PCR fragments collectively, producing fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR can also be used for genotyping by conventional PCR methods and in detection experiments utilizing methods similar to loop-mediated isothermal amplification (LAMP).

STITCHER can deal with each single sequence and multi-sequence enter, and particular options facilitate quite a few different PCR purposes, together with meeting PCR, adapter PCR, and primer strolling. Discipline PCR, and particularly, LAMP, provides promise as an on web site software for pathogen detection in underdeveloped areas, and STITCHER contains off-target detection options for pathogens generally focused utilizing LAMP know-how.

Primer design utilizing Primer Specific® for SYBR Inexperienced-based quantitative PCR.

To quantitate the gene expression, real-time RT-PCR or quantitative PCR (qPCR) is among the most delicate, dependable, and generally used strategies in molecular biology. The reliability and success of a real-time PCR assay depend upon the optimum experiment design. Primers are crucial constituents of real-time PCR experiments similar to in SYBR Inexperienced-based detection assays.

Designing of an acceptable and particular primer pair is extraordinarily essential for proper estimation of transcript abundance of any gene in a given pattern.

SYBR Green qPCR Master Mix (No ROX)
C0006-50mg	TargetMol Chemicals	50mg	Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)
C0006-5mg	TargetMol Chemicals	5mg	Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)
HY-K0523	MedChemExpress	5 mL (500 rxns )	EUR 308.4

SYBR Green qPCR Master Mix (Low ROX)
C0008-10mg	TargetMol Chemicals	10mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-1g	TargetMol Chemicals	1g	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-1mg	TargetMol Chemicals	1mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-50mg	TargetMol Chemicals	50mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-5mg	TargetMol Chemicals	5mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522	MedChemExpress	5 mL (500 rxns )	EUR 308.4

SYBR Green qPCR Master Mix (High ROX)
C0007-10mg	TargetMol Chemicals	10mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-1g	TargetMol Chemicals	1g	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-1mg	TargetMol Chemicals	1mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-50mg	TargetMol Chemicals	50mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-5mg	TargetMol Chemicals	5mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
HY-K0521	MedChemExpress	1 mL (100 rxns)	EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)
B21702	Bimake	5 ml	EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21703	Bimake	25 ml	EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21402	Bimake	5 ml	EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21403	Bimake	25 ml	EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix
Q111-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR SYBR® Green Master Mix
Q111-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-25	ApexBio	25ml	EUR 336

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-5	ApexBio	5ml	EUR 96

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-50	ApexBio	50ml	EUR 672

ig SYBR Green qPCR 2X Master Mix - 200 Reactions
3354	Intact Genomics	200	EUR 110
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-25	ApexBio	25ml	EUR 336
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-5	ApexBio	5ml	EUR 96
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-50	ApexBio	50ml	EUR 672
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns
THK0221	Westburg	each	Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns
THK0222	Westburg	each	Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns
THK0223	Westburg	each	EUR 3324.5

Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns
THK0241	Westburg	each	Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns
THK0242	Westburg	each	Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns
THK0243	Westburg	each	Ask for price

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns
THK0251	Westburg	each	EUR 232.17

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns
THK0252	Westburg	each	EUR 1046.4

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns
THK0253	Westburg	each	Ask for price

BioEasy Master Mix (SYBR Green)
TRI-B25L1	TRI Biotech	200T	EUR 403.2

ChamQ SYBR qPCR Master Mix
Q311-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix
Q311-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

ChamQ SYBR qPCR Master Mix
Q311-00-100rxns	Vazyme	100 rxns	EUR 1.09

ChamQ SYBR qPCR Master Mix
Q311-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix
Q311-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

Apex qPCR GREEN Master Mix
42-116PG	Genesee Scientific	400 x 25µl Reactions/Unit	EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix
42-117PG	Genesee Scientific	4000 x 25µl Reactions/Unit	EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix
42-118PG	Genesee Scientific	400 x 25µl Reactions/Unit	EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix
42-119PG	Genesee Scientific	4000 x 25µl Reactions/Unit	EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix
42-120PG	Genesee Scientific	400 x 25µl Reactions/Unit	EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix
42-121PG	Genesee Scientific	4000 x 25µl Reactions/Unit	EUR 3095.57
Description: 2X, High ROX, 50ml

BioEasy Master Mix (SYBR Green, No ROX)
TRI-B25L1D	TRI Biotech	200T	EUR 403.2

BioEasy Master Mix (SYBR Green, low ROX)
TRI-B25L1C	TRI Biotech	200T	EUR 403.2

BioEasy Master Mix (SYBR Green, high ROX)
TRI-B25L1B	TRI Biotech	200T	EUR 403.2

ChamQ SYBR Color qPCR Master Mix
Q411-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix
Q411-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR Color qPCR Master Mix
Q411-00-100rxns	Vazyme	100 rxns	EUR 1.09

ChamQ SYBR Color qPCR Master Mix
Q411-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix
Q411-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

AceQ Universal SYBR qPCR Master Mix
Q511-02	Vazyme	500 rxn (20 μl/rxn)	EUR 152.72

AceQ Universal SYBR qPCR Master Mix
Q511-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 688.5

AceQ Universal SYBR qPCR Master Mix
Q511-00-100rxns	Vazyme	100 rxns	EUR 1.09

AceQ Universal SYBR qPCR Master Mix
Q511-02-500rxns	Vazyme	500 rxns	EUR 158.14

AceQ Universal SYBR qPCR Master Mix
Q511-03-2500rxns	Vazyme	2,500 rxns	EUR 712.94

miRNA Universal SYBR qPCR Master Mix
MQ101-01	Vazyme	125 rxn(20 μl/rxn)	EUR 38.9

miRNA Universal SYBR qPCR Master Mix
MQ101-02	Vazyme	500 rxn(20 μl/rxn)	EUR 145

ChamQ Universal SYBR qPCR Master Mix
Q711-02	Vazyme	500 rxn (20 μl/rxn)	EUR 152.72

ChamQ Universal SYBR qPCR Master Mix
Q711-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 688.5

miRNA Universal SYBR qPCR Master Mix
MQ101-00-100rxns	Vazyme	100 rxns	EUR 1.09

miRNA Universal SYBR qPCR Master Mix
MQ101-01-125rxns	Vazyme	125 rxns	EUR 40.28

miRNA Universal SYBR qPCR Master Mix
MQ101-02-500rxns	Vazyme	500 rxns	EUR 150.15

ChamQ Universal SYBR qPCR Master Mix
Q711-00-100rxns	Vazyme	100 rxns	EUR 1.09

ChamQ Universal SYBR qPCR Master Mix
Q711-02-500rxns	Vazyme	500 rxns	EUR 158.14

ChamQ Universal SYBR qPCR Master Mix
Q711-03-2500rxns	Vazyme	2,500 rxns	EUR 712.94

2×SYBR Green qPCR Mix
MBS8579840-200Tests	MyBiosource	200Tests	EUR 285

2×SYBR Green qPCR Mix
MBS8579840-50Tests	MyBiosource	50Tests	EUR 180

2×SYBR Green qPCR Mix
MBS8579840-5x200Tests	MyBiosource	5x200Tests	EUR 1230

2×SYBR Green qPCR Mix
MBS8579840-5x50Tests	MyBiosource	5x50Tests	EUR 770

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

Taq Pro Universal SYBR qPCR Master Mix
Q712-02	Vazyme	500 rxns (20 μl/rxn)	EUR 161

Taq Pro Universal SYBR qPCR Master Mix
Q712-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 725

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

Taq Pro Universal SYBR qPCR Master Mix
Q712-00-100rxns	Vazyme	100 rxns	EUR 1.09

Taq Pro Universal SYBR qPCR Master Mix
Q712-02-500rxns	Vazyme	500 rxns	EUR 166.72

Taq Pro Universal SYBR qPCR Master Mix
Q712-03-2500rxns	Vazyme	2,500 rxns	EUR 750.74

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns
QLMM10	Vivantis	each	Ask for price

HotStart™ 2X FAST Green qPCR Master Mix
K1171-25	ApexBio	25ml	EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix
K1171-5	ApexBio	5ml	EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix
K1171-50	ApexBio	50ml	EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-25	ApexBio	25ml	EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-5	ApexBio	5ml	EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-50	ApexBio	50ml	EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns
QLMM12	Vivantis	each	Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns
QLMM17	Vivantis	each	Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns
QLMM02	Vivantis	each	Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns
QLMM07	Vivantis	each	Ask for price

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

ABsolute SYBR Green QPCR Mix 800 rxns
AB-1158/B	Westburg	each	Ask for price

ABsolute SYBR Green QPCR Mix 200 rxns
AB-1159	Westburg	each	EUR 529.74

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17274	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17274-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17275	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17275-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-200Tests	MyBiosource	200Tests	EUR 285

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-50Tests	MyBiosource	50Tests	EUR 180

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-5x200Tests	MyBiosource	5x200Tests	EUR 1230

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-5x50Tests	MyBiosource	5x50Tests	EUR 770

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Genious 2X SYBR Green Fast qPCR Mix
RK21204	Abclonal	1 mL	EUR 328.5

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Right here, we’re presenting a fast, simple, and dependable technique for designing target-specific primers utilizing Primer Specific(®) software program for real-time PCR (qPCR) experiments.