Analytical PCR experiments ideally use inside probes for monitoring the amplification response and particular detection of the amplicon. Such inside probes must be designed in shut context with the amplification primers, and will require further issues for the detection of genetic variations.
Right here we describe Edesign, a brand new on-line and stand-alone software for designing units of PCR primers along with an inside probe for conducting quantitative real-time PCR (qPCR) and genotypic experiments. Edesign can be utilized for choosing commonplace DNA oligonucleotides like as an example TaqMan probes, however has been additional prolonged with new features and enhanced design options for Eprobes.
Eprobes, with their single thiazole orange-labelled nucleotide, permit for extremely delicate genotypic assays due to their increased DNA binding affinity as in comparison with commonplace DNA oligonucleotides. Utilizing new thermodynamic parameters, Edesign considers distinctive options of Eprobes throughout primer and probe design for establishing qPCR experiments and genotyping by melting curve evaluation.
Design and analysis of reverse transcription nested PCR primers for the detection of betanodavirus in finfish.
Viral encephalopathy and retinopathy in any other case generally known as viral nervous necrosis (VNN) is a neuropathological situation affecting greater than 50 fish species worldwide, largely marine.
Completely different PCR protocols with particular primers had been reported from many international locations for affirmation of VNN in fishes. Within the current examine, two pairs of primers had been designed and evaluated for the prognosis of medical and subclinical circumstances of infections from subject.
These primers designated as BARL-F1/BARL-R1 amplified a 902 bp product within the variable area (T4) of the coat protein gene by first step PCR. Nested PCR primers BARL-F2/BARL-R2 amplified a fraction of 313 bp. The outcomes had been comparable with different generally used primer units similar to F2/R3 and RG668f/RG919r primers.
These new primers might detect betanodavirus in commonplace reference samples containing low, reasonable and excessive viral load. Identified constructive and unfavourable management samples of fish additionally revealed a predictive worth of 100 % by RT-PCR prognosis.
MRPrimer: a MapReduce-based technique for the thorough design of legitimate and ranked primers for PCR.
Primer design is a elementary method that’s extensively used for polymerase chain response (PCR). Though many strategies have been proposed for primer design, they require quite a lot of handbook effort to generate possible and legitimate primers, together with homology exams on off-target sequences utilizing BLAST-like instruments.
That strategy is inconvenient for a lot of goal sequences of quantitative PCR (qPCR) attributable to contemplating the identical stringent and allele-invariant constraints. To deal with this problem, we suggest a completely new technique referred to as MRPrimer that may design all possible and legitimate primer pairs present in a DNA database directly, whereas concurrently checking a mess of filtering constraints and validating primer specificity.
Moreover, MRPrimer suggests the very best primer pair for every goal sequence, primarily based on a rating technique. By way of qPCR evaluation utilizing 343 primer pairs and the corresponding sequencing and comparative analyses, we confirmed that the primer pairs designed by MRPrimer are very steady and efficient for qPCR.
As well as, MRPrimer is computationally environment friendly and scalable and due to this fact helpful for rapidly developing a complete assortment of possible and legitimate primers for steadily up to date databases like RefSeq. Moreover, we propose that MRPrimer could be utilized conveniently for experiments requiring primer design, particularly real-time qPCR.
STITCHER: An internet useful resource for high-throughput design of primers for overlapping PCR purposes.
Overlapping PCR is routinely utilized in a large variety of molecular purposes. These embody stitching PCR fragments collectively, producing fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR can also be used for genotyping by conventional PCR methods and in detection experiments utilizing methods similar to loop-mediated isothermal amplification (LAMP).
STITCHER can deal with each single sequence and multi-sequence enter, and particular options facilitate quite a few different PCR purposes, together with meeting PCR, adapter PCR, and primer strolling. Discipline PCR, and particularly, LAMP, provides promise as an on web site software for pathogen detection in underdeveloped areas, and STITCHER contains off-target detection options for pathogens generally focused utilizing LAMP know-how.
Primer design utilizing Primer Specific® for SYBR Inexperienced-based quantitative PCR.
To quantitate the gene expression, real-time RT-PCR or quantitative PCR (qPCR) is among the most delicate, dependable, and generally used strategies in molecular biology. The reliability and success of a real-time PCR assay depend upon the optimum experiment design. Primers are crucial constituents of real-time PCR experiments similar to in SYBR Inexperienced-based detection assays.
Designing of an acceptable and particular primer pair is extraordinarily essential for proper estimation of transcript abundance of any gene in a given pattern.
SYBR Green qPCR Master Mix (No ROX) |
C0006-50mg |
TargetMol Chemicals |
50mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
C0006-5mg |
TargetMol Chemicals |
5mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix (Low ROX) |
C0008-10mg |
TargetMol Chemicals |
10mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
C0008-1g |
TargetMol Chemicals |
1g |
Ask for price |
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
C0008-1mg |
TargetMol Chemicals |
1mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
C0008-50mg |
TargetMol Chemicals |
50mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
C0008-5mg |
TargetMol Chemicals |
5mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
SYBR Green qPCR Master Mix (High ROX) |
C0007-10mg |
TargetMol Chemicals |
10mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
C0007-1g |
TargetMol Chemicals |
1g |
Ask for price |
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
C0007-1mg |
TargetMol Chemicals |
1mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
C0007-50mg |
TargetMol Chemicals |
50mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
C0007-5mg |
TargetMol Chemicals |
5mg |
Ask for price |
Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
2x SYBR Green qPCR Master Mix (Low ROX) |
B21702 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21402 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
AceQ qPCR SYBR® Green Master Mix |
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-25 |
ApexBio |
25ml |
EUR 336 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-5 |
ApexBio |
5ml |
EUR 96 |
HotStart™ 2X SYBR Green qPCR Master Mix |
K1070-50 |
ApexBio |
50ml |
EUR 672 |
ig SYBR Green qPCR 2X Master Mix - 200 Reactions |
3354 |
Intact Genomics |
200 |
EUR 110 |
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification. |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-25 |
ApexBio |
25ml |
EUR 336 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-5 |
ApexBio |
5ml |
EUR 96 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
K1170-50 |
ApexBio |
50ml |
EUR 672 |
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
ChamQ SYBR qPCR Master Mix |
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix |
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
Apex qPCR GREEN Master Mix |
42-116PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
Description: 2X, Without ROX, 5ml |
Apex qPCR GREEN Master Mix |
42-117PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
Description: 2X, Without ROX, 50ml |
Apex qPCR GREEN Master Mix |
42-118PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 338.88 |
Description: 2X, Low ROX, 5ml |
Apex qPCR GREEN Master Mix |
42-119PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
Description: 2X, Low ROX, 50ml |
Apex qPCR GREEN Master Mix |
42-121PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 3095.57 |
Description: 2X, High ROX, 50ml |
Apex qPCR GREEN Master Mix |
42-120PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
Description: 2X, High ROX, 5ml |
ChamQ SYBR Color qPCR Master Mix |
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix |
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
miRNA Universal SYBR qPCR Master Mix |
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 38.9 |
miRNA Universal SYBR qPCR Master Mix |
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 145 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 161 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 725 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
QLMM10 |
Vivantis |
each |
Ask for price |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
QLMM12 |
Vivantis |
each |
Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns |
QLMM17 |
Vivantis |
each |
Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
QLMM02 |
Vivantis |
each |
Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns |
QLMM07 |
Vivantis |
each |
Ask for price |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17270 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17271 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17272 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17273 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17274 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17275 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 203 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 508 |
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
EUR 328.5 |
|
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
EUR 76.5 |
|
ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns |
QLMM12-R |
Vivantis |
each |
Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns |
QLMM17-R |
Vivantis |
each |
Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX, 150rxns |
QLMM02-R |
Vivantis |
each |
Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II with ROX, 150rxns |
QLMM07-R |
Vivantis |
each |
Ask for price |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 897 |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 224 |
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns |
QLMM12-LR |
Vivantis |
each |
Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns |
QLMM17-LR |
Vivantis |
each |
Ask for price |
BrightCycle Universal SYBR Green qPCR Mix with UDG |
RK21219 |
Abclonal |
5mL |
EUR 475 |
ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX, 150rxns |
QLMM02-LR |
Vivantis |
each |
Ask for price |
ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns |
QLMM07-LR |
Vivantis |
each |
Ask for price |
PCR Master mix (SYBR) |
abx460031-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
PCR Master mix (SYBR) |
abx460031-596tests |
Abbexa |
5 × 96 tests |
Ask for price |
PCR Master mix (SYBR) |
abx460031-96tests |
Abbexa |
96 tests |
EUR 337.5 |
Genious 2X SYBR Green Fast qPCR Mix (No ROX) |
RK21205 |
Abclonal |
1 ml |
EUR 328.5 |
|
qPCR Master Mix |
abx460014-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
qPCR Master Mix |
abx460014-596tests |
Abbexa |
5 × 96 tests |
Ask for price |
qPCR Master Mix |
abx460014-96tests |
Abbexa |
96 tests |
EUR 337.5 |
1ml PrecisionPLUS qPCR Master Mix premixed with SYBRgreen |
Z-PPLUS-SY-1ML |
Novacyt Group |
1ml |
EUR 81 |
Description: Precision®PLUS |
Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed) |
RK21206 |
Abclonal |
1 ml |
EUR 328.5 |
|
Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed) |
RK21207 |
Abclonal |
1 ml |
EUR 93 |
|
1ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
Z-PFAST-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
Z-PFAST-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-001 |
GenDepot |
1ml |
EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-002 |
GenDepot |
2x1ml |
EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-005 |
GenDepot |
5x1ml |
EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-010 |
GenDepot |
10x1ml |
EUR 1632 |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I, 150rxns |
QLMM14 |
Vivantis |
each |
Ask for price |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II, 150rxns |
QLMM18 |
Vivantis |
each |
Ask for price |
Maximo Dry-Master Mix (Lyophilized qPCR Mastermix with Sybrgreen) |
S295RS |
GeneOn |
192 rcs (20µl, in plates or tubes) |
EUR 171 |
ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I, 150rxns |
QLMM04 |
Vivantis |
each |
Ask for price |
ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II, 150rxns |
QLMM08 |
Vivantis |
each |
Ask for price |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
1ml PrecisionPLUS qPCR Master Mix |
Z-PPLUS-1ML |
Novacyt Group |
1ml |
EUR 81 |
Description: Precision®PLUS |
1-Step 2X RT-PCR Master Mix-SYBR Green-HY (High Yield) |
W141 |
101Bio |
200 rxn |
EUR 199 |
Apex qPCR Probe Master Mix |
42-116P |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
Description: 2X, Without ROX, 5ml |
Apex qPCR Probe Master Mix |
42-117P |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
Description: 2X, Without ROX, 50ml |
Apex qPCR Probe Master Mix |
42-118P |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
Description: 2X, Low ROX, 5ml |
Apex qPCR Probe Master Mix |
42-119P |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
Description: 2X, Low ROX, 50ml |
Apex qPCR Probe Master Mix |
42-120P |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
Description: 2X, High ROX, 5ml |
Apex qPCR Probe Master Mix |
42-121P |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
Description: 2X, High ROX, 50ml |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 120.55 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 543.5 |
ViPrimePLUS Taq qPCR Master Mix, 150rxns |
QLMM11 |
Vivantis |
each |
Ask for price |
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
ViPrimePLUS AtTaq qPCR Master Mix, 150rxns |
QLMM01 |
Vivantis |
each |
Ask for price |
1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility) |
W142 |
101Bio |
200 rxn |
EUR 199 |
1ml Precision FAST qPCR Master Mix |
Z-PFAST-1ML |
Novacyt Group |
1ml |
EUR 90 |
Description: Precision®FAST |
2ml Precision FAST qPCR Master Mix |
Z-PFAST-2ML |
Novacyt Group |
2ml |
EUR 161 |
Description: Precision®FAST |
PanGreen™ Universal SYBR® Green Mastermix |
QSD01-0100 |
Bio-Helix |
100 rxns |
EUR 20 |
PanGreen™ Universal SYBR Green Mastermix UNG |
QSD02-0100 |
Bio-Helix |
100 rxns |
EUR 22 |
1ml PrecisionPLUS CL qPCR Master Mix |
Z-PPLUS-CL-1ML |
Novacyt Group |
1ml |
EUR 81 |
Description: Precision®PLUS |
1ml PrecisionPLUS LR qPCR Master Mix |
Z-PPLUS-LR-1ML |
Novacyt Group |
1ml |
EUR 81 |
Description: Precision®PLUS |
ViPrimePLUS Lyophilized qPCR Master Mix, 150rxns |
QLMM05 |
Vivantis |
each |
Ask for price |
Entrans 2X qPCR Probe Master Mix |
RK21208 |
Abclonal |
40 RXN |
EUR 119 |
|
Right here, we’re presenting a fast, simple, and dependable technique for designing target-specific primers utilizing Primer Specific(®) software program for real-time PCR (qPCR) experiments.