Analytical PCR experiments ideally use inside probes for monitoring the amplification response and particular detection of the amplicon. Such inside probes must be designed in shut context with the amplification primers, and will require further issues for the detection of genetic variations.
Right here we describe Edesign, a brand new on-line and stand-alone software for designing units of PCR primers along with an inside probe for conducting quantitative real-time PCR (qPCR) and genotypic experiments. Edesign can be utilized for choosing commonplace DNA oligonucleotides like as an example TaqMan probes, however has been additional prolonged with new features and enhanced design options for Eprobes.
Eprobes, with their single thiazole orange-labelled nucleotide, permit for extremely delicate genotypic assays due to their increased DNA binding affinity as in comparison with commonplace DNA oligonucleotides. Utilizing new thermodynamic parameters, Edesign considers distinctive options of Eprobes throughout primer and probe design for establishing qPCR experiments and genotyping by melting curve evaluation.
Design and analysis of reverse transcription nested PCR primers for the detection of betanodavirus in finfish.
Viral encephalopathy and retinopathy in any other case generally known as viral nervous necrosis (VNN) is a neuropathological situation affecting greater than 50 fish species worldwide, largely marine.
Completely different PCR protocols with particular primers had been reported from many international locations for affirmation of VNN in fishes. Within the current examine, two pairs of primers had been designed and evaluated for the prognosis of medical and subclinical circumstances of infections from subject.
These primers designated as BARL-F1/BARL-R1 amplified a 902 bp product within the variable area (T4) of the coat protein gene by first step PCR. Nested PCR primers BARL-F2/BARL-R2 amplified a fraction of 313 bp. The outcomes had been comparable with different generally used primer units similar to F2/R3 and RG668f/RG919r primers.
These new primers might detect betanodavirus in commonplace reference samples containing low, reasonable and excessive viral load. Identified constructive and unfavourable management samples of fish additionally revealed a predictive worth of 100 % by RT-PCR prognosis.
MRPrimer: a MapReduce-based technique for the thorough design of legitimate and ranked primers for PCR.
Primer design is a elementary method that’s extensively used for polymerase chain response (PCR). Though many strategies have been proposed for primer design, they require quite a lot of handbook effort to generate possible and legitimate primers, together with homology exams on off-target sequences utilizing BLAST-like instruments.

That strategy is inconvenient for a lot of goal sequences of quantitative PCR (qPCR) attributable to contemplating the identical stringent and allele-invariant constraints. To deal with this problem, we suggest a completely new technique referred to as MRPrimer that may design all possible and legitimate primer pairs present in a DNA database directly, whereas concurrently checking a mess of filtering constraints and validating primer specificity.
Moreover, MRPrimer suggests the very best primer pair for every goal sequence, primarily based on a rating technique. By way of qPCR evaluation utilizing 343 primer pairs and the corresponding sequencing and comparative analyses, we confirmed that the primer pairs designed by MRPrimer are very steady and efficient for qPCR.
As well as, MRPrimer is computationally environment friendly and scalable and due to this fact helpful for rapidly developing a complete assortment of possible and legitimate primers for steadily up to date databases like RefSeq. Moreover, we propose that MRPrimer could be utilized conveniently for experiments requiring primer design, particularly real-time qPCR.
STITCHER: An internet useful resource for high-throughput design of primers for overlapping PCR purposes.
Overlapping PCR is routinely utilized in a large variety of molecular purposes. These embody stitching PCR fragments collectively, producing fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR can also be used for genotyping by conventional PCR methods and in detection experiments utilizing methods similar to loop-mediated isothermal amplification (LAMP).
STITCHER can deal with each single sequence and multi-sequence enter, and particular options facilitate quite a few different PCR purposes, together with meeting PCR, adapter PCR, and primer strolling. Discipline PCR, and particularly, LAMP, provides promise as an on web site software for pathogen detection in underdeveloped areas, and STITCHER contains off-target detection options for pathogens generally focused utilizing LAMP know-how.
Primer design utilizing Primer Specific® for SYBR Inexperienced-based quantitative PCR.
To quantitate the gene expression, real-time RT-PCR or quantitative PCR (qPCR) is among the most delicate, dependable, and generally used strategies in molecular biology. The reliability and success of a real-time PCR assay depend upon the optimum experiment design. Primers are crucial constituents of real-time PCR experiments similar to in SYBR Inexperienced-based detection assays.
Designing of an acceptable and particular primer pair is extraordinarily essential for proper estimation of transcript abundance of any gene in a given pattern.
2x SYBR Green qPCR Master Mix (High ROX) |
B21402 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
B21702 |
Bimake |
5 ml |
EUR 268.8 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 290.4 |
2X Universal SYBR Green Fast qPCR Mix |
RK21203 |
Abclonal |
1 mL |
Ask for price |
|
Genious 2X SYBR Green Fast qPCR Mix |
RK21204 |
Abclonal |
1 mL |
Ask for price |
|
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
RK21205 |
Abclonal |
1 ml |
Ask for price |
|
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
Entrans 2X qPCR Probe Master Mix |
RK21208 |
Abclonal |
40 RXN |
Ask for price |
|
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17270 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
17271 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17272 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
17273 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17274 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
17275 |
AAT Bioquest |
5 mL |
EUR 308 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-001 |
GenDepot |
1ml |
EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-002 |
GenDepot |
2x1ml |
EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-005 |
GenDepot |
5x1ml |
EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-010 |
GenDepot |
10x1ml |
EUR 1632 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 789.36 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 197.34 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17276 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
17277 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17278 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
17279 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17280 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
17281 |
AAT Bioquest |
5 mL |
EUR 308 |
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
HS-100-QPCR |
CORNING |
100/pk |
EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
HS-150-QPCR |
CORNING |
100/pk |
EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
SYBR Green I |
B1747-25 |
Biovision |
each |
EUR 1396.8 |
SYBR Green I |
B1747-5 |
Biovision |
each |
EUR 464.4 |
Fast EvaGreen Master Mix for qPCR(200 rxn) |
31003 |
Biotium |
2x1ML |
EUR 244.8 |
Description: Minimum order quantity: 1 unit of 2x1ML |
Fast EvaGreen Master Mix for qPCR (500 rxn) |
31003-1 |
Biotium |
5x1ML |
EUR 463.2 |
Description: Minimum order quantity: 1 unit of 5x1ML |
Fast EvaGreen Master Mix for qPCR (5000 rxn) |
31003-2 |
Biotium |
50x1ML |
EUR 3739.2 |
Description: Minimum order quantity: 1 unit of 50x1ML |
Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
31020-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
31020-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
RT Master Mix for qPCR (gDNA digester plus) |
HY-K0511 |
MedChemExpress |
1 mL (100 rxns) |
EUR 378 |
ABScript II One Step SYBR Green RT-qPCR Kit |
RK20404 |
Abclonal |
10 RXN |
Ask for price |
|
Jade? Master Mix |
M1105-500 |
Biovision |
each |
EUR 529.2 |
Taqman Master Mix |
M1121-500 |
Biovision |
each |
EUR 529.2 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
31041-1 |
Biotium |
5x1mL |
EUR 326.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
31041-20mL |
Biotium |
2x10mL |
EUR 592.8 |
Description: Minimum order quantity: 1 unit of 2x10mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
31041-T |
Biotium |
1mL |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1mL |
EZSolution? SYBR Green I |
B1748-1 |
Biovision |
each |
EUR 744 |
EZSolution? SYBR Green I |
B1748-500 |
Biovision |
each |
EUR 516 |
OPTIONAL GREEN FILTER (572NM) |
GDBL-GREEN |
CORNING |
1/pk |
EUR 1262.4 |
Description: Lab Equipment; Axygen Branded EQ |
Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
31003-T |
Biotium |
1ML |
EUR 147.6 |
Description: Minimum order quantity: 1 unit of 1ML |
Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
31020-T |
Biotium |
1mL |
EUR 160.8 |
Description: Minimum order quantity: 1 unit of 1mL |
CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation |
CRISPY100A-1 |
SBI |
200 rxn |
EUR 412 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17282 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17283 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17286 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17287 |
AAT Bioquest |
5 mL |
EUR 308 |
Green qPCR SuperMix |
20-abx098031 |
Abbexa |
-
EUR 1245.60
-
EUR 526.80
-
EUR 760.80
|
|
|
Green Taq Mix |
P131-01 |
Vazyme |
5 ml |
EUR 142.8 |
Green Taq Mix |
P131-02 |
Vazyme |
15 ml |
EUR 180 |
Green Taq Mix |
P131-03 |
Vazyme |
50 ml |
EUR 297.6 |
amfiSure PCR Master Mix |
P0311-010 |
GenDepot |
2x50 rxns |
EUR 151.2 |
amfiSure PCR Master Mix |
P0311-025 |
GenDepot |
5X50 rxns |
EUR 198 |
amfiSure PCR Master Mix |
P0311-050 |
GenDepot |
10X50 rxns |
EUR 262.8 |
amfiSure PCR Master Mix |
P0311-100 |
GenDepot |
10x1ml |
EUR 343.2 |
amfiSure PCR Master Mix |
P0311-125 |
GenDepot |
15X50 rxns |
EUR 333.6 |
amfiSure PCR Master Mix |
P0311-200 |
GenDepot |
20x1ml |
EUR 562.8 |
amfiSure PCR Master Mix |
P0311-250 |
GenDepot |
50x50 rxns |
EUR 902.4 |
amfiSure PCR Master Mix |
P0311-500 |
GenDepot |
100x50 rxns |
EUR 1509.6 |
amfiXpand PCR Master Mix |
P0331-010 |
GenDepot |
2X50 rxns |
EUR 181.2 |
amfiXpand PCR Master Mix |
P0331-025 |
GenDepot |
5X50 rxns |
EUR 327.6 |
amfiXpand PCR Master Mix |
P0331-050 |
GenDepot |
10X50 rxns |
EUR 537.6 |
amfiXpand PCR Master Mix |
P0331-250 |
GenDepot |
50x50 rxns |
EUR 2247.6 |
2 × Taq Master Mix |
P111-01 |
Vazyme |
5 ml |
EUR 140.4 |
2 × Taq Master Mix |
P111-02 |
Vazyme |
15 ml |
EUR 175.2 |
2 × Taq Master Mix |
P111-03 |
Vazyme |
50 ml |
EUR 283.2 |
2 × AceTaq Master Mix |
P411-01 |
Vazyme |
1 ml |
EUR 142.8 |
2 × AceTaq Master Mix |
P411-02 |
Vazyme |
5 ml |
EUR 189.6 |
2 × AceTaq Master Mix |
P411-03 |
Vazyme |
15 ml |
EUR 314.4 |
Taqman Master Mix-iCycler |
M1122-500 |
Biovision |
each |
EUR 529.2 |
Taqman Master Mix-ROX |
M1124-500 |
Biovision |
each |
EUR 529.2 |
Taqman Master Mix-Multiplex |
M1125-500 |
Biovision |
each |
EUR 529.2 |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
31014 |
Biotium |
2x1mL |
EUR 273.6 |
Description: Minimum order quantity: 1 unit of 2x1mL |
Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
31014-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
31014-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
31015-1 |
Biotium |
5x1mL |
EUR 524.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
31015-2 |
Biotium |
50x1mL |
EUR 4291.2 |
Description: Minimum order quantity: 1 unit of 50x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
31042-1 |
Biotium |
5x1mL |
EUR 326.4 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
31042-20mL |
Biotium |
2x10mL |
EUR 592.8 |
Description: Minimum order quantity: 1 unit of 2x10mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
31042-T |
Biotium |
1mL |
EUR 136.8 |
Description: Minimum order quantity: 1 unit of 1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
9-31045 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
9-31046 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
S190 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
S190L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291 |
AAT Bioquest |
5 mL |
EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293 |
AAT Bioquest |
5 mL |
EUR 308 |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-372S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
EUR 1362.00
-
EUR 543.60
-
EUR 794.40
|
|
|
Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
EUR 994.80
-
EUR 260.40
-
EUR 1362.00
-
EUR 493.20
|
|
|
Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
|
|
|
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-373L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-373S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-374L |
MiTeGen |
10 x 1,25 ml |
EUR 720.29 |
|
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-374S |
MiTeGen |
2 x 1,25 ml |
EUR 180.14 |
|
Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-375L |
MiTeGen |
10 x 1,25 ml |
EUR 864.5 |
|
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
M-PCR-375S |
MiTeGen |
2 x 1,25 ml |
EUR 216.19 |
|
Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
Seamless cloning Master Mix (Kit) |
B632219 |
Bio Basic |
40RXN, 40prep |
EUR 319.96 |
|
amfiSure Prime PCR Master Mix |
P1311-025 |
GenDepot |
5X50 rxns |
EUR 193.2 |
amfiSure Prime PCR Master Mix |
P1311-050 |
GenDepot |
10X50 rxns |
EUR 262.8 |
amfiSure Prime PCR Master Mix |
P1311-125 |
GenDepot |
15X50 rxns |
EUR 327.6 |
amfiSure Prime PCR Master Mix |
P1311-500 |
GenDepot |
100x50 rxns |
EUR 1485.6 |
2 × Taq Plus Master Mix |
P211-01 |
Vazyme |
5 ml |
EUR 160.8 |
2 × Taq Plus Master Mix |
P211-02 |
Vazyme |
15 ml |
EUR 231.6 |
2 × Taq Plus Master Mix |
P211-03 |
Vazyme |
50 ml |
EUR 466.8 |
2 × Rapid Taq Master Mix |
P222-01 |
Vazyme |
5 ml (5×1ml) |
EUR 144 |
2 × Rapid Taq Master Mix |
P222-02 |
Vazyme |
15 ml (15×1ml) |
EUR 183.6 |
2 × Rapid Taq Master Mix |
P222-03 |
Vazyme |
50 ml (50 x 1 ml) |
EUR 308.4 |
2 × Rapid Taq Master Mix |
P222-04 |
Vazyme |
50 ml (10×5ml) |
EUR 308.4 |
amfiSure Advanced PCR Master Mix |
P2311-025 |
GenDepot |
5X50 rxns |
EUR 198 |
amfiSure Advanced PCR Master Mix |
P2311-050 |
GenDepot |
10X50 rxns |
EUR 267.6 |
amfiSure Advanced PCR Master Mix |
P2311-125 |
GenDepot |
15X50 rxns |
EUR 338.4 |
amfiSure Advanced PCR Master Mix |
P2311-250 |
GenDepot |
50x50 rxns |
EUR 902.4 |
amfiSure Advanced PCR Master Mix |
P2311-500 |
GenDepot |
100x50 rxns |
EUR 1534.8 |
2 × Vazyme LAmp Master Mix |
P311-01 |
Vazyme |
1 ml |
EUR 164.4 |
2 × Vazyme LAmp Master Mix |
P311-02 |
Vazyme |
5 ml |
EUR 254.4 |
Right here, we’re presenting a fast, simple, and dependable technique for designing target-specific primers utilizing Primer Specific(®) software program for real-time PCR (qPCR) experiments.