Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays.

Analytical PCR experiments ideally use inside probes for monitoring the amplification response and particular detection of the amplicon. Such inside probes must be designed in shut context with the amplification primers, and will require further issues for the detection of genetic variations.

Right here we describe Edesign, a brand new on-line and stand-alone software for designing units of PCR primers along with an inside probe for conducting quantitative real-time PCR (qPCR) and genotypic experiments. Edesign can be utilized for choosing commonplace DNA oligonucleotides like as an example TaqMan probes, however has been additional prolonged with new features and enhanced design options for Eprobes.

Eprobes, with their single thiazole orange-labelled nucleotide, permit for extremely delicate genotypic assays due to their increased DNA binding affinity as in comparison with commonplace DNA oligonucleotides. Utilizing new thermodynamic parameters, Edesign considers distinctive options of Eprobes throughout primer and probe design for establishing qPCR experiments and genotyping by melting curve evaluation.

Design and analysis of reverse transcription nested PCR primers for the detection of betanodavirus in finfish.

Viral encephalopathy and retinopathy in any other case generally known as viral nervous necrosis (VNN) is a neuropathological situation affecting greater than 50 fish species worldwide, largely marine.

Completely different PCR protocols with particular primers had been reported from many international locations for affirmation of VNN in fishes. Within the current examine, two pairs of primers had been designed and evaluated for the prognosis of medical and subclinical circumstances of infections from subject.

These primers designated as BARL-F1/BARL-R1 amplified a 902 bp product within the variable area (T4) of the coat protein gene by first step PCR. Nested PCR primers BARL-F2/BARL-R2 amplified a fraction of 313 bp. The outcomes had been comparable with different generally used primer units similar to F2/R3 and RG668f/RG919r primers.

These new primers might detect betanodavirus in commonplace reference samples containing low, reasonable and excessive viral load. Identified constructive and unfavourable management samples of fish additionally revealed a predictive worth of 100 % by RT-PCR prognosis.

MRPrimer: a MapReduce-based technique for the thorough design of legitimate and ranked primers for PCR.

Primer design is a elementary method that’s extensively used for polymerase chain response (PCR). Though many strategies have been proposed for primer design, they require quite a lot of handbook effort to generate possible and legitimate primers, together with homology exams on off-target sequences utilizing BLAST-like instruments.

That strategy is inconvenient for a lot of goal sequences of quantitative PCR (qPCR) attributable to contemplating the identical stringent and allele-invariant constraints. To deal with this problem, we suggest a completely new technique referred to as MRPrimer that may design all possible and legitimate primer pairs present in a DNA database directly, whereas concurrently checking a mess of filtering constraints and validating primer specificity.

Moreover, MRPrimer suggests the very best primer pair for every goal sequence, primarily based on a rating technique. By way of qPCR evaluation utilizing 343 primer pairs and the corresponding sequencing and comparative analyses, we confirmed that the primer pairs designed by MRPrimer are very steady and efficient for qPCR.

As well as, MRPrimer is computationally environment friendly and scalable and due to this fact helpful for rapidly developing a complete assortment of possible and legitimate primers for steadily up to date databases like RefSeq. Moreover, we propose that MRPrimer could be utilized conveniently for experiments requiring primer design, particularly real-time qPCR.

STITCHER: An internet useful resource for high-throughput design of primers for overlapping PCR purposes.

Overlapping PCR is routinely utilized in a large variety of molecular purposes. These embody stitching PCR fragments collectively, producing fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR can also be used for genotyping by conventional PCR methods and in detection experiments utilizing methods similar to loop-mediated isothermal amplification (LAMP).

STITCHER can deal with each single sequence and multi-sequence enter, and particular options facilitate quite a few different PCR purposes, together with meeting PCR, adapter PCR, and primer strolling. Discipline PCR, and particularly, LAMP, provides promise as an on web site software for pathogen detection in underdeveloped areas, and STITCHER contains off-target detection options for pathogens generally focused utilizing LAMP know-how.

Primer design utilizing Primer Specific® for SYBR Inexperienced-based quantitative PCR.

To quantitate the gene expression, real-time RT-PCR or quantitative PCR (qPCR) is among the most delicate, dependable, and generally used strategies in molecular biology. The reliability and success of a real-time PCR assay depend upon the optimum experiment design. Primers are crucial constituents of real-time PCR experiments similar to in SYBR Inexperienced-based detection assays.

Designing of an acceptable and particular primer pair is extraordinarily essential for proper estimation of transcript abundance of any gene in a given pattern.

AceQ qPCR SYBR® Green Master Mix
Q111-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

AceQ qPCR SYBR® Green Master Mix
Q111-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

2x SYBR Green qPCR Master Mix (High ROX)
B21402	Bimake	5 ml	EUR 224
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21403	Bimake	25 ml	EUR 856
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21702	Bimake	5 ml	EUR 224
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21703	Bimake	25 ml	EUR 856
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

ig SYBR Green qPCR 2X Master Mix - 500 Reactions
3356	Intact Genomics	1/EA	EUR 288

AceQ Universal SYBR qPCR Master Mix
Q511-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

AceQ Universal SYBR qPCR Master Mix
Q511-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

ChamQ Universal SYBR qPCR Master Mix
Q711-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

ChamQ Universal SYBR qPCR Master Mix
Q711-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

miRNA Universal SYBR® qPCR Master Mix
MQ101-01	Vazyme	125 rxn(20 μl/rxn)	EUR 138

miRNA Universal SYBR® qPCR Master Mix
MQ101-02	Vazyme	500 rxn(20 μl/rxn)	EUR 242

2X Universal SYBR Green Fast qPCR Mix
RK21203	Abclonal	1 mL	Ask for price

Genious 2X SYBR Green Fast qPCR Mix
RK21204	Abclonal	1 mL	Ask for price

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)
RK21205	Abclonal	1 ml	Ask for price

RT Master Mix for qPCR
HY-K0510	MedChemExpress	1 mL (100 rxns)	EUR 291

AceQ qPCR Probe Master Mix
Q112-02	Vazyme	500 rxn (20 μl/rxn)	EUR 221

AceQ qPCR Probe Master Mix
Q112-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 646

Entrans 2X qPCR Probe Master Mix
RK21208	Abclonal	40 RXN	Ask for price

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001	GenDepot	1ml	EUR 280

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-002	GenDepot	2x1ml	EUR 482

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-005	GenDepot	5x1ml	EUR 828

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-010	GenDepot	10x1ml	EUR 1360

HotStart PCR Master mix (2X, Green Dye)
BS9291	Bio Basic	5X1mL, 5ml	EUR 209.5

HotStart PCR Master mix (2X, Green Dye)
BS9292	Bio Basic	1ml	EUR 96.4

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR	CORNING	100/pk	EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR	CORNING	100/pk	EUR 318
Description: Sealing Products; Sealing films - Axygen

ABScript II One Step SYBR Green RT-qPCR Kit
RK20404	Abclonal	10 RXN	Ask for price

SYBR Green I
B1747-25	Biovision		EUR 1164

SYBR Green I
B1747-5	Biovision		EUR 387

RT Master Mix for qPCR (gDNA digester plus)
HY-K0511	MedChemExpress	1 mL (100 rxns)	EUR 315

Fast EvaGreen Master Mix for qPCR(200 rxn)
31003	Biotium	2x1ML	EUR 204
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)
31003-1	Biotium	5x1ML	EUR 386
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)
31003-2	Biotium	50x1ML	EUR 3116
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (200 rxn)
31020	Biotium	2x1mL	EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)
31020-1	Biotium	5x1mL	EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2	Biotium	50x1mL	EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Genorise® 2 x qPCR HotStart Master Mix
GR108002	Genorise Scientific	2.0 ml	EUR 279

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)
31041-1	Biotium	5x1mL	EUR 272
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)
31041-20mL	Biotium	2x10mL	EUR 494
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)
31041-T	Biotium	1mL	EUR 114
Description: Minimum order quantity: 1 unit of 1mL

Green qPCR SuperMix
20-abx098031	Abbexa	EUR 1038.00 EUR 439.00 EUR 634.00	15 ml 1 ml 5 ml

EZSolution? SYBR Green I
B1748-1	Biovision		EUR 620

EZSolution? SYBR Green I
B1748-500	Biovision		EUR 430

OPTIONAL GREEN FILTER (572NM)
GDBL-GREEN	CORNING	1/pk	EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Jade? Master Mix
M1105-500	Biovision		EUR 441

Taqman Master Mix
M1121-500	Biovision		EUR 441

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)
31003-T	Biotium	1ML	EUR 123
Description: Minimum order quantity: 1 unit of 1ML

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)
31020-T	Biotium	1mL	EUR 134
Description: Minimum order quantity: 1 unit of 1mL

Green Taq Mix
P131-01	Vazyme	5 ml	EUR 119

Green Taq Mix
P131-02	Vazyme	15 ml	EUR 150

Green Taq Mix
P131-03	Vazyme	50 ml	EUR 248

Green qPCR SuperMix UDG
20-abx098032	Abbexa	EUR 1135.00 EUR 453.00 EUR 662.00	15 ml 1 ml 5 ml

Top Green qPCR SuperMix
20-abx098033	Abbexa	EUR 829.00 EUR 217.00 EUR 1135.00 EUR 411.00	15 ml 1 ml 25 ml 5 ml

Tip Green qPCR SuperMix
20-abx098034	Abbexa	EUR 968.00 EUR 230.00 EUR 1330.00 EUR 467.00	15 ml 1 ml 25 ml 5 ml

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials
S190	GeneOn	100 rcs (2,5 ml)	EUR 138

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials
S190L	GeneOn	500 rcs (12,5 ml)	EUR 523

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)
31014	Biotium	2x1mL	EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)
31014-1	Biotium	5x1mL	EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
31014-2	Biotium	50x1mL	EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)
31015-1	Biotium	5x1mL	EUR 437
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2	Biotium	50x1mL	EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)
31042-1	Biotium	5x1mL	EUR 272
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)
31042-20mL	Biotium	2x10mL	EUR 494
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)
31042-T	Biotium	1mL	EUR 114
Description: Minimum order quantity: 1 unit of 1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
9-31045	Biotium	EUR 114.00 EUR 482.00 EUR 266.00	1mL 1X10mL 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)
9-31046	Biotium	EUR 114.00 EUR 482.00 EUR 266.00	1mL 1X10mL 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

amfiSure PCR Master Mix
P0311-010	GenDepot	2x50 rxns	EUR 126

amfiSure PCR Master Mix
P0311-025	GenDepot	5X50 rxns	EUR 165

amfiSure PCR Master Mix
P0311-050	GenDepot	10X50 rxns	EUR 219

amfiSure PCR Master Mix
P0311-100	GenDepot	10x1ml	EUR 286

amfiSure PCR Master Mix
P0311-125	GenDepot	15X50 rxns	EUR 278

amfiSure PCR Master Mix
P0311-200	GenDepot	20x1ml	EUR 469

amfiSure PCR Master Mix
P0311-250	GenDepot	50x50 rxns	EUR 752

amfiSure PCR Master Mix
P0311-500	GenDepot	100x50 rxns	EUR 1258

amfiXpand PCR Master Mix
P0331-010	GenDepot	2X50 rxns	EUR 151

amfiXpand PCR Master Mix
P0331-025	GenDepot	5X50 rxns	EUR 273

amfiXpand PCR Master Mix
P0331-050	GenDepot	10X50 rxns	EUR 448

amfiXpand PCR Master Mix
P0331-250	GenDepot	50x50 rxns	EUR 1873

Accuris Taq Master Mix
PR1001-1000	Benchmark Scientific	1 PC	EUR 300.26

Accuris Taq Master Mix
PR1001-200	Benchmark Scientific	1 PC	EUR 121.62

Accuris Taq Master Mix
PR1001-S	Benchmark Scientific	1 PC	EUR 69.79

Taqman Master Mix-iCycler
M1122-500	Biovision		EUR 441

Taqman Master Mix-ROX
M1124-500	Biovision		EUR 441

Taqman Master Mix-Multiplex
M1125-500	Biovision		EUR 441

2 × Taq Master Mix
P111-01	Vazyme	5 ml	EUR 117

2 × Taq Master Mix
P111-02	Vazyme	15 ml	EUR 146

2 × Taq Master Mix
P111-03	Vazyme	50 ml	EUR 236

2 × AceTaq Master Mix
P411-01	Vazyme	1 ml	EUR 119

2 × AceTaq Master Mix
P411-02	Vazyme	5 ml	EUR 158

2 × AceTaq Master Mix
P411-03	Vazyme	15 ml	EUR 262

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles
RA910A-1	SBI	20 profiles	EUR 1108

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials
S150	GeneOn	100 rcs (2,5 ml)	EUR 138

qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials
S150L	GeneOn	500 rcs (12,5 ml)	EUR 523

qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials
S200	GeneOn	100 rcs (2,5 ml)	EUR 159

qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials
S200L	GeneOn	500 rcs (12,5 ml)	EUR 612

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T	Biotium	1mL	EUR 134
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
31015	Biotium	2x1mL	EUR 228
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)
31015-T	Biotium	1mL	EUR 134
Description: Minimum order quantity: 1 unit of 1mL

HotTaq Probe qPCR Mix (ROX)
BT11001	Bioatlas	250rxn	EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)
BT11003	Bioatlas	250rxn	EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix
BT11004	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
BT11101	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)
BT11103	Bioatlas	250rxn	EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix
BT11104	Bioatlas	250rxn	EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

amfiSure Prime PCR Master Mix
P1311-025	GenDepot	5X50 rxns	EUR 161

amfiSure Prime PCR Master Mix
P1311-050	GenDepot	10X50 rxns	EUR 219

amfiSure Prime PCR Master Mix
P1311-125	GenDepot	15X50 rxns	EUR 273

amfiSure Prime PCR Master Mix
P1311-500	GenDepot	100x50 rxns	EUR 1238

amfiSure Advanced PCR Master Mix
P2311-025	GenDepot	5X50 rxns	EUR 165

amfiSure Advanced PCR Master Mix
P2311-050	GenDepot	10X50 rxns	EUR 223

amfiSure Advanced PCR Master Mix
P2311-125	GenDepot	15X50 rxns	EUR 282

amfiSure Advanced PCR Master Mix
P2311-250	GenDepot	50x50 rxns	EUR 752

amfiSure Advanced PCR Master Mix
P2311-500	GenDepot	100x50 rxns	EUR 1279

amfiRivert cDNA Synthesis Master Mix
R5101-050	GenDepot	50 rxns	EUR 415

amfiRivert cDNA Synthesis Master Mix
R5101-100	GenDepot	2X50 rxns	EUR 847

amfiRivert cDNA Synthesis Master Mix
R5101-200	GenDepot	4X50 rxns	EUR 1211

Seamless cloning Master Mix (Kit)
B632219	Bio Basic	40RXN, 40prep	EUR 266.63

Accuris Taq Plus Master Mix
PR1001-TP-1000	Benchmark Scientific	1 PC	EUR 827.41

Accuris Taq Plus Master Mix
PR1001-TP-200	Benchmark Scientific	1 PC	EUR 242.84

Accuris Taq Plus Master Mix
PR1001-TP-S	Benchmark Scientific	1 PC	EUR 69.79

Taqman Master Mix-Low ROX
M1123-500	Biovision		EUR 441

2 × Taq Plus Master Mix
P211-01	Vazyme	5 ml	EUR 134

2 × Taq Plus Master Mix
P211-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix
P211-03	Vazyme	50 ml	EUR 389

2 × Rapid Taq Master Mix
P222-01	Vazyme	5 ml (5×1ml)	EUR 120

2 × Rapid Taq Master Mix
P222-02	Vazyme	15 ml (15×1ml)	EUR 153

2 × Rapid Taq Master Mix
P222-03	Vazyme	50 ml (50 x 1 ml)	EUR 257

2 × Rapid Taq Master Mix
P222-04	Vazyme	50 ml (10×5ml)	EUR 257

2 × Vazyme LAmp Master Mix
P311-01	Vazyme	1 ml	EUR 137

2 × Vazyme LAmp Master Mix
P311-02	Vazyme	5 ml	EUR 212

2 × Vazyme LAmp Master Mix
P311-03	Vazyme	15 ml	EUR 401

2 × Phanta Max Master Mix
P515-01	Vazyme	1 ml	EUR 145

2 × Phanta Max Master Mix
P515-02	Vazyme	5 ml	EUR 269

2 × Phanta Max Master Mix
P515-03	Vazyme	15 ml	EUR 575

AceQ U+ Probe Master Mix
Q113-02	Vazyme	500 rxn (20 μl/rxn)	EUR 267

AceQ U+ Probe Master Mix
Q113-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 842

qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials
S160	GeneOn	100 rcs (2,5 ml)	EUR 159

qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials
S160L	GeneOn	500 rcs (12,5 ml)	EUR 612

SYBR Green I Nucleic Acid Gel Stain
HY-K1004	MedChemExpress	100 μL	EUR 113

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P	Yeastern Biotech	100 Preps 1.25 ml	Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-400P	Yeastern Biotech	400 Preps 1.25 ml x 4	Ask for price

SensiFAST SYBR Lo-ROX Mix, 50 mL
BIO-94050	Bioline	5000 rxn	Ask for price

SensiFAST SYBR No-ROX Mix, 50 mL
BIO-98050	Bioline	5000 rxn	Ask for price

Right here, we’re presenting a fast, simple, and dependable technique for designing target-specific primers utilizing Primer Specific(®) software program for real-time PCR (qPCR) experiments.