PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.

PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.

We just lately demonstrated that the Escherichia coli ribosome is strong sufficient to accommodate international 16S rRNAs from numerous gamma- and betaproteobacteria micro organism (Kitahara et al., 2012).

Therein, we used the widespread common primers Bac8f and UN1541r to acquire a virtually full-length gene. Nevertheless, we observed that these primers overlap variable websites at 19[A/C] and 1527[U/C] in Bac8f and UN1541r, respectively, and thus, the amplicon might include mutations.

That is problematic, notably for the previous website, as a result of the 19th nucleotide pairs with the 916th nucleotide, which is part of the “central pseudoknot” and is important for perform. Due to this fact, we mutationally investigated the function of the bottom pair utilizing a number of 16S rRNAs from gamma- and betaproteobacteria.

We discovered that each the native base pairs (gammaproteobacterial 19A-916U and betaproteobacterial 19C-916G) and the non-native 19A-916G pair retained perform, whereas the non-native 19C-916U was faulty 16S rRNAs. We subsequent designed a brand new primer set, Bac1f and UN1542r, in order that they don’t overlap the potential mismatch websites. 16S rRNA amplicons obtained from the environmental metagenome utilizing the brand new primer set had been dominated by proteobacterial species (~85%).

Subsequent purposeful screening recognized varied 16S rRNAs from proteobacteria, all of which contained native 19A-916U or 19C-916G base pairs. The primers developed on this examine are thus advantageous for purposeful characterization of international 16S rRNA in E. coli with no artifacts.

STITCHER 2.0: primer design for overlapping PCR purposes.

Overlapping polymerase chain response (PCR) is a standard method utilized by researchers in very numerous fields that allows the consumer to ‘sew’ particular person items of DNA collectively. Beforehand, we have now reported an online primarily based device referred to as STITCHER that gives a platform for researchers to automate the design of primers for overlapping PCR purposes.

Right here we current STITCHER 2.0, which represents a considerable replace to STITCHER. STITCHER 2.Zero is a newly designed net device that automates the design of primers for overlapping PCR. In contrast to STITCHER, STITCHER 2.Zero considers numerous algorithmic parameters, and returns a number of end result information that embrace a facility for the consumer to attract their very own primers in addition to complete visible guides to the consumer’s enter, output, and designed primers. These end result information present better management and perception throughout experimental design and troubleshooting.

PrimerSuite: A Excessive-Throughput Net-Based mostly Primer Design Program for Multiplex Bisulfite PCR.

The evaluation of DNA methylation at CpG dinucleotides has turn into a significant analysis focus on account of its regulatory function in quite a few organic processes, however the requisite want for assays which amplify bisulfite-converted DNA represents a significant bottleneck as a result of distinctive design constraints imposed on bisulfite-PCR primers.

PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.

Furthermore, a overview of the literature indicated no out there software program options which accommodated each high-throughput primer design, help for multiplex amplification assays, and primer-dimer prediction. In response, the tri-modular software program package deal PrimerSuite was developed to help bisulfite multiplex PCR purposes.

This software program was constructed to (i) design bisulfite primers towards a number of areas concurrently (PrimerSuite), (ii) display screen for primer-primer dimerizing artefacts (PrimerDimer), and (iii) help multiplex PCR assays (PrimerPlex). Furthermore, a significant focus within the growth of this software program package deal was the emphasis on intensive empirical validation, and over 1300 distinctive primer pairs have been efficiently designed and screened, with over 94% of them producing amplicons of the anticipated measurement, and a mean mapping effectivity of 93% when screened utilizing bisulfite multiplex resequencing. The potential use of the software program in different bisulfite-based purposes akin to methylation-specific PCR is into consideration for future updates.

PrimerMapper: excessive throughput primer design and graphical meeting for PCR and SNP detection.

Primer design represents a extensively employed gambit in numerous molecular purposes together with PCR, sequencing, and probe hybridization. Variations of PCR, together with primer strolling, allele-specific PCR, and nested PCR present specialised validation and detection protocols for molecular analyses that always require screening giant numbers of DNA fragments.

In these instances, automated sequence retrieval and processing turn into vital options, and moreover, a graphic that gives the consumer with a visible information to the distribution of designed primers throughout targets is most useful in rapidly ascertaining primer protection. To this finish, I describe right here, PrimerMapper, which supplies a complete graphical consumer interface that designs strong primers from any variety of inputted sequences whereas offering the consumer with each, graphical maps of primer distribution for every inputted sequence, and likewise a world assembled map of all inputted sequences with designed primers.

PrimerMapper additionally allows the visualization of graphical maps inside a browser and permits the consumer to attract new primers immediately onto the webpage.

Power Eva QPCR SuperMix Kit

K5057400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Green qPCR SuperMix

20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Probe qPCR SuperMix

20-abx098040
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Tip Green qPCR SuperMix

20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 230.00
  • EUR 411.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 408
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 594
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml
  • Shipped within 5-10 working days.

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 718
  • Shipped within 5-10 working days.

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 235

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 235

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 293

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 244

PCR SuperMix

20-abx098887
  • EUR 272.00
  • EUR 578.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

PCR SuperMix (-dye)

20-abx098002
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

PCR SuperMix (+dye)

20-abx098003
  • EUR 411.00
  • EUR 189.00
  • EUR 6174.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml
  • Shipped within 5-10 working days.

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Plus PCR SuperMix

20-abx098888
  • EUR 244.00
  • EUR 467.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Library Amplification SuperMix

20-abx098894
  • EUR 258.00
  • EUR 537.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 141

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

qPCR Control Template Kit

G929 100 rxns
EUR 148

QPCR Positive Control Kit

M1126-100
EUR 403

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns Ask for price

COLLECTION CONTAINER 1L, EVA FILM

91-200-01 1/pk
EUR 80
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 2L, EVA FILM

91-200-02 1/pk
EUR 88
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-05 1/pk
EUR 99
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-10 1/pk
EUR 101
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-20 1/pk
EUR 110
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-36 1/pk
EUR 86
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-39 1/pk
EUR 89
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 1L, EVA FILM

91-200-42 1/pk
EUR 73
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-43 1/pk
EUR 151
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-45 1/pk
EUR 126
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-47 1/pk
EUR 132
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 50L, EVA FILM

91-200-48 1/pk
EUR 146
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 100L, EVA FILM

91-200-82 1/pk
EUR 279
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 200L, EVA FILM

91-200-83 1/pk
EUR 292
Description: Flexible Containers; Bioprocess Bags and Parts

T PCR SuperMix (-dye)

20-abx098005
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

T PCR SuperMix (+dye)

20-abx098006
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 244.00
  • EUR 495.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2
  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

COLLECTION CONTAINER 500 ML, EVA FILM

91-200-41 1/pk
EUR 69
Description: Flexible Containers; Bioprocess Bags and Parts

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

igScript RT-qPCR Kit - 50 Reactions

4513 1/EA
EUR 248

igScript RT-qPCR Kit - 200 Reactions

4515 1/EA
EUR 716

Coronavirus Rapid RT-qPCR Detection Kit

K1461-100 100 Rxns
EUR 551

Adeno-Associated Virus qPCR Quantification Kit

K1473-100 100 Rxns
EUR 348

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110
  • Category: Cas9

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999
  • Category: Lineage Profilers

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172
  • Category: MicroRNA Tools

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 880
  • Category: Long Non-coding RNA Tools

Protein Eva-1 Homolog C (EVA1C) Antibody

20-abx302696
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 376
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 572
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 732
  • Shipped within 5-10 working days.

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 648
  • Shipped within 5-10 working days.

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
  • EUR 495.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
  • EUR 592.00
  • EUR 1024.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • EUR 718.00
  • EUR 578.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 10 x 1,25 ml
EUR 1719

COVID-19 RT-qPCR Rapid Detection Kit

G628 NULL
EUR 0

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 20 reactions
EUR 331
  • Category: PiggyBac Technology

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-iCycler

MasterMix-5PC 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex

MasterMix-5PM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex

MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 646

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 578
  • Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 606
  • Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 759
  • Shipped within 5-10 working days.

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 885
  • Shipped within 5-10 working days.

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)

105-520 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)

105-522 10 x 1,25 ml
EUR 1719

igScript One Step RT-qPCR Kit - 100 Reactions

4214 1/EA
EUR 253

igScript One Step RT-qPCR Kit - 500 Reactions

4218 1/EA
EUR 773

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 428

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 394

2x SYBR Green qPCR Master Mix

B21202 5 mL
EUR 224
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix

B21203 25 mL
EUR 856
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

HotTaq Probe qPCR Mix (no ROX)

BT11002 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)

BT11102 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Universal miRNA qPCR Primer (10 µM)

abx298006-200ul 200 ul
EUR 189
  • Shipped within 5-10 working days.

Opti-Seal qPCR and Storage Films

G495 100 Films/Box
EUR 202

Opti-Seal qPCR 384 Well Plate

G935 10 Plates/Box
EUR 89

Opti-Seal qPCR 384 Well Plate

G936 100 Plates/Case
EUR 428

BrightGreen 5X qPCR MasterMix-Low ROX

MasterMix-5L 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Low ROX

MasterMix-5PL 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-No Dye

MasterMix-5PS 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-No Dye

MasterMix-5S 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen Express 2X qPCR MasterMix-iCycler

MasterMix-EC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen Express 2X qPCR MasterMix-ROX

MasterMix-ER 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-Low ROX

MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-No Dye

MasterMix-KS 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-No Dye

MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-Low ROX

MasterMix-PL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-No Dye

MasterMix-PS 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-No Dye

MasterMix-S 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-No Dye

MasterMix-S-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 221

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 646

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 221

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 646

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2152
  • Category: Cas9
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 445
  • Category: Cas9

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • EUR 217.00
  • EUR 356.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 606
  • Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 662
  • Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 857
  • Shipped within 5-10 working days.

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 801
  • Shipped within 5-10 working days.

Green One-Step qRT-PCR SuperMix (GC Rich)

20-abx09803920ulSystems
  • EUR 592.00
  • EUR 1024.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Probe One-Step qRT-PCR SuperMix (GC Rich)

20-abx09804120ulSystems
  • EUR 495.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1024.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

One.Step RT-qPCR Probe Kit with HighROX (2X concentrated)

105-510 2 x 1,25 ml
EUR 440

One.Step RT-qPCR Probe Kit with HighROX (2X concentrated)

105-512 10 x 1,25 ml
EUR 1628

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1152
  • Category: Stem Cell Products

ROX reference dye for qPCR, 500 uL

31110 500 uL
EUR 46

ROX reference dye for qPCR, 1 mL

41110 1 mL
EUR 52

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 113

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 257

Different options of PrimerMapper embrace allele-specific design options for SNP genotyping, a distant BLAST window to NCBI databases, and distant sequence retrieval from GenBank and dbSNP. PrimerMapper is hosted at GitHub and freely out there with out restriction.

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