PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.

We just lately demonstrated that the Escherichia coli ribosome is strong sufficient to accommodate international 16S rRNAs from numerous gamma- and betaproteobacteria micro organism (Kitahara et al., 2012).

Therein, we used the widespread common primers Bac8f and UN1541r to acquire a virtually full-length gene. Nevertheless, we observed that these primers overlap variable websites at 19[A/C] and 1527[U/C] in Bac8f and UN1541r, respectively, and thus, the amplicon might include mutations.

That is problematic, notably for the previous website, as a result of the 19th nucleotide pairs with the 916th nucleotide, which is part of the “central pseudoknot” and is important for perform. Due to this fact, we mutationally investigated the function of the bottom pair utilizing a number of 16S rRNAs from gamma- and betaproteobacteria.

We discovered that each the native base pairs (gammaproteobacterial 19A-916U and betaproteobacterial 19C-916G) and the non-native 19A-916G pair retained perform, whereas the non-native 19C-916U was faulty 16S rRNAs. We subsequent designed a brand new primer set, Bac1f and UN1542r, in order that they don’t overlap the potential mismatch websites. 16S rRNA amplicons obtained from the environmental metagenome utilizing the brand new primer set had been dominated by proteobacterial species (~85%).

Subsequent purposeful screening recognized varied 16S rRNAs from proteobacteria, all of which contained native 19A-916U or 19C-916G base pairs. The primers developed on this examine are thus advantageous for purposeful characterization of international 16S rRNA in E. coli with no artifacts.

STITCHER 2.0: primer design for overlapping PCR purposes.

Overlapping polymerase chain response (PCR) is a standard method utilized by researchers in very numerous fields that allows the consumer to ‘sew’ particular person items of DNA collectively. Beforehand, we have now reported an online primarily based device referred to as STITCHER that gives a platform for researchers to automate the design of primers for overlapping PCR purposes.

Right here we current STITCHER 2.0, which represents a considerable replace to STITCHER. STITCHER 2.Zero is a newly designed net device that automates the design of primers for overlapping PCR. In contrast to STITCHER, STITCHER 2.Zero considers numerous algorithmic parameters, and returns a number of end result information that embrace a facility for the consumer to attract their very own primers in addition to complete visible guides to the consumer’s enter, output, and designed primers. These end result information present better management and perception throughout experimental design and troubleshooting.

PrimerSuite: A Excessive-Throughput Net-Based mostly Primer Design Program for Multiplex Bisulfite PCR.

The evaluation of DNA methylation at CpG dinucleotides has turn into a significant analysis focus on account of its regulatory function in quite a few organic processes, however the requisite want for assays which amplify bisulfite-converted DNA represents a significant bottleneck as a result of distinctive design constraints imposed on bisulfite-PCR primers.

Furthermore, a overview of the literature indicated no out there software program options which accommodated each high-throughput primer design, help for multiplex amplification assays, and primer-dimer prediction. In response, the tri-modular software program package deal PrimerSuite was developed to help bisulfite multiplex PCR purposes.

This software program was constructed to (i) design bisulfite primers towards a number of areas concurrently (PrimerSuite), (ii) display screen for primer-primer dimerizing artefacts (PrimerDimer), and (iii) help multiplex PCR assays (PrimerPlex). Furthermore, a significant focus within the growth of this software program package deal was the emphasis on intensive empirical validation, and over 1300 distinctive primer pairs have been efficiently designed and screened, with over 94% of them producing amplicons of the anticipated measurement, and a mean mapping effectivity of 93% when screened utilizing bisulfite multiplex resequencing. The potential use of the software program in different bisulfite-based purposes akin to methylation-specific PCR is into consideration for future updates.

PrimerMapper: excessive throughput primer design and graphical meeting for PCR and SNP detection.

Primer design represents a extensively employed gambit in numerous molecular purposes together with PCR, sequencing, and probe hybridization. Variations of PCR, together with primer strolling, allele-specific PCR, and nested PCR present specialised validation and detection protocols for molecular analyses that always require screening giant numbers of DNA fragments.

In these instances, automated sequence retrieval and processing turn into vital options, and moreover, a graphic that gives the consumer with a visible information to the distribution of designed primers throughout targets is most useful in rapidly ascertaining primer protection. To this finish, I describe right here, PrimerMapper, which supplies a complete graphical consumer interface that designs strong primers from any variety of inputted sequences whereas offering the consumer with each, graphical maps of primer distribution for every inputted sequence, and likewise a world assembled map of all inputted sequences with designed primers.

PrimerMapper additionally allows the visualization of graphical maps inside a browser and permits the consumer to attract new primers immediately onto the webpage.

Pro QPCR SuperMix Kit
K5053400	Biochain	400 reactions	EUR 326

Green qPCR SuperMix
20-abx098031	Abbexa	Ask for price Ask for price Ask for price	15 ml 1 ml 5 ml

Probe qPCR SuperMix
20-abx098040	Abbexa	Ask for price Ask for price Ask for price	15 ml 1 ml 5 ml

Green qPCR SuperMix
abx098031-100l	Abbexa	100 µl	EUR 675

Green qPCR SuperMix
abx098031-200l	Abbexa	200 µl	EUR 912.5

Pro QPCR SuperMix Kit - ROX premixed
K5056200	Biochain	200 reactions	EUR 180

Pro QPCR SuperMix Kit - ROX premixed
K5056400	Biochain	400 reactions	EUR 326

Green qPCR SuperMix UDG
20-abx098032	Abbexa	Ask for price Ask for price Ask for price	15 ml 1 ml 5 ml

Top Green qPCR SuperMix
20-abx098033	Abbexa	Ask for price Ask for price Ask for price Ask for price	15 ml 1 ml 25 ml 5 ml

Tip Green qPCR SuperMix
20-abx098034	Abbexa	Ask for price Ask for price Ask for price Ask for price	15 ml 1 ml 25 ml 5 ml

Green qPCR SuperMix UDG
abx098032-100l	Abbexa	100 µl	EUR 687.5

Green qPCR SuperMix UDG
abx098032-200l	Abbexa	200 µl	EUR 912.5

Top Green qPCR SuperMix
abx098033-100l	Abbexa	100 µl	EUR 262.5

Top Green qPCR SuperMix
abx098033-200l	Abbexa	200 µl	EUR 487.5

Tip Green qPCR SuperMix
abx098034-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix
abx098034-200l	Abbexa	200 µl	EUR 437.5

Fast Pro QPCR SuperMix Kit - ROX premixed
K5058200	Biochain	200 reactions	EUR 211

Fast Pro QPCR SuperMix Kit - ROX premixed
K5058400	Biochain	400 reactions	EUR 354

Library Quantification qPCR SuperMix
20-abx098896	Abbexa	Ask for price Ask for price	1 ml 5 ml

Library Quantification qPCR SuperMix
abx098896-100l	Abbexa	100 µl	EUR 700

Library Quantification qPCR SuperMix
abx098896-200l	Abbexa	200 µl	EUR 962.5

EnTurbo™ probe qPCR SuperMix
EQ017-25mL	ELK Biotech	25mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-5mL	ELK Biotech	5mL	EUR 78

EnTurbo™ probe qPCR SuperMix
EQ017	ELK Biotech	5mL	EUR 240

Top Green qPCR SuperMix (+Dye II)
20-abx098890	Abbexa	Ask for price Ask for price Ask for price Ask for price	15 ml 1 ml 25 ml 5 ml

Tip Green qPCR SuperMix (+Dye II)
20-abx098891	Abbexa	Ask for price Ask for price Ask for price Ask for price	15 ml 1 ml 25 ml 5 ml

Top Green qPCR SuperMix (+Dye II)
abx098890-100l	Abbexa	100 µl	EUR 262.5

Top Green qPCR SuperMix (+Dye II)
abx098890-200l	Abbexa	200 µl	EUR 487.5

Tip Green qPCR SuperMix (+Dye II)
abx098891-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix (+Dye II)
abx098891-200l	Abbexa	200 µl	EUR 437.5

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix
EQ036	ELK Biotech	5mL	EUR 340

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	Ask for price Ask for price	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems

HyperScript RT SuperMix for qPCR
K1074-100	ApexBio	100 rxn (20 uL/rxn)	EUR 280
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR
K1074-50	ApexBio	50 rxn (20 uL/rxn)	EUR 150
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

cDNA Synthesis SuperMix for qPCR
abx098019-100l	Abbexa	100 µl	EUR 825

cDNA Synthesis SuperMix for qPCR
abx098019-1ml	Abbexa	1 ml	EUR 2387.5

cDNA Synthesis SuperMix for qPCR
abx098019-200l	Abbexa	200 µl	EUR 1000

HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035	ELK Biotech	5mL	EUR 340

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 861.6

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-100l	Abbexa	100 µl	EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-200l	Abbexa	200 µl	Ask for price

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01	Vazyme	100rxns(20µl/rxn)	EUR 326

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01-100rxns	Vazyme	100 rxns	EUR 337.36

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 217.5

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-100l	Abbexa	100 µl	EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-1ml	Abbexa	1 ml	Ask for price

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-200l	Abbexa	200 µl	Ask for price

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01-100rxns	Vazyme	100 rxns	EUR 225.22

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034	ELK Biotech	5mL	EUR 340

HyperScript RT SuperMix for qPCR (with gDNA wiper)
K1174-100	ApexBio	100 rxns	EUR 304
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR (with gDNA wiper)
K1174-50	ApexBio	50 rxns	EUR 176
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HiScript II Q RT SuperMix for qPCR (+gDNA wiper)
R223-01	Vazyme	100rxns(20µl/rxn)	EUR 159.5

HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 159.5

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054200	Biochain	200 reactions	EUR 468

QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054400	Biochain	400 reactions	EUR 711

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01	Vazyme	100 rxns (21 μl/rxn)	EUR 217.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01-100rxns	Vazyme	100 rxns	EUR 225.22

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054002	Biochain	200 rxn	EUR 468

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit
K5054004	Biochain	400 rxn	EUR 711

PCR SuperMix
20-abx098887	Abbexa	Ask for price Ask for price	1 ml 5 ml

2X PCR SuperMix
MB200-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

2X PCR SuperMix
MBS402025-100Reactions	MyBiosource	100Reactions	EUR 175

2X PCR SuperMix
MBS402025-4Reactions	MyBiosource	4Reactions	EUR 160

PCR SuperMix (-dye)
20-abx098002	Abbexa	Ask for price Ask for price Ask for price	15 ml 1 ml 5 ml

PCR SuperMix (+dye)
20-abx098003	Abbexa	Ask for price Ask for price Ask for price Ask for price	15 ml 1 ml 480 ml 5 ml

PCR SuperMix (-dye)
abx098002-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (-dye)
abx098002-200l	Abbexa	200 µl	EUR 337.5

PCR SuperMix (+dye)
abx098003-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (+dye)
abx098003-200l	Abbexa	200 µl	EUR 337.5

Plus PCR SuperMix
20-abx098888	Abbexa	Ask for price Ask for price	1 ml 5 ml

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Plus PCR SuperMix
abx098888-100l	Abbexa	100 µl	EUR 300

Plus PCR SuperMix
abx098888-200l	Abbexa	200 µl	EUR 612.5

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

Probe Direct SuperMix
abx461057-1096tests	Abbexa	10 × 96 tests	Ask for price

Probe Direct SuperMix
abx461057-596tests	Abbexa	5 × 96 tests	EUR 2800

Probe Direct SuperMix
abx461057-96tests	Abbexa	96 tests	EUR 812.5

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA Synthesis SuperMix
abx098014-100l	Abbexa	100 µl	EUR 725

cDNA Synthesis SuperMix
abx098014-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix
abx098014-200l	Abbexa	200 µl	EUR 812.5

T PCR SuperMix (-dye)
20-abx098005	Abbexa	Ask for price Ask for price	1 ml 5 ml

T PCR SuperMix (+dye)
20-abx098006	Abbexa	Ask for price Ask for price	1 ml 5 ml

T PCR SuperMix (-dye)
abx098005-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (-dye)
abx098005-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (+dye)
abx098006-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (+dye)
abx098006-200l	Abbexa	200 µl	EUR 462.5

AmpHS 2X PCR SuperMix
MB203-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	Ask for price Ask for price	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	Ask for price Ask for price	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
abx098010-100l	Abbexa	100 µl	EUR 300

FastPfu PCR SuperMix (-dye)
abx098010-200l	Abbexa	200 µl	EUR 575

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055200	Biochain	200 reactions	EUR 427

QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055400	Biochain	400 reactions	EUR 682

Library Amplification SuperMix
20-abx098894	Abbexa	Ask for price Ask for price	1 ml 5 ml

Library Amplification SuperMix
abx098894-100l	Abbexa	100 µl	EUR 737.5

Library Amplification SuperMix
abx098894-200l	Abbexa	200 µl	EUR 1137.5

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 106.8

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 169.2

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	Ask for price Ask for price Ask for price	15 ml 1 ml 5 ml

PCR SuperMix for PAGE (+dye)
abx098004-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix for PAGE (+dye)
abx098004-200l	Abbexa	200 µl	EUR 337.5

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055002	Biochain	200 rxn	EUR 426

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit
K5055004	Biochain	400 rxn	EUR 682

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

OmniPCR Supermix w Fluorescent dye
MBA01-0100	Bio-Helix	100 rxns (2.5ml)	EUR 16

EnTurbo™ SYBR Green PCR SuperMix
EQ001-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Green PCR SuperMix
EQ001	ELK Biotech	5mL	EUR 340

Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 777.6

Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix
20-abx09804220ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green Two-Step qRT-PCR SuperMix
abx098035-100l	Abbexa	100 µl	EUR 925

Green Two-Step qRT-PCR SuperMix
abx098035-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-200l	Abbexa	200 µl	Ask for price

Green One-Step qRT-PCR SuperMix
abx098038-100l	Abbexa	100 µl	EUR 737.5

Green One-Step qRT-PCR SuperMix
abx098038-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix
abx098038-200l	Abbexa	200 µl	EUR 1050

Probe One-Step qRT-PCR SuperMix
abx098042-100l	Abbexa	100 µl	EUR 850

Probe One-Step qRT-PCR SuperMix
abx098042-1ml	Abbexa	1 ml	Ask for price

Probe One-Step qRT-PCR SuperMix
abx098042-200l	Abbexa	200 µl	EUR 1400

AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X)
MB204-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 13

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 910.8

Two-Step RT-PCR SuperMix (12 kb)
abx098025-100l	Abbexa	100 µl	EUR 887.5

Two-Step RT-PCR SuperMix (12 kb)
abx098025-1ml	Abbexa	1 ml	Ask for price

Two-Step RT-PCR SuperMix (12 kb)
abx098025-200l	Abbexa	200 µl	Ask for price

Two-Step RT-PCR SuperMix (15 kb)
abx098026-100l	Abbexa	100 µl	EUR 1087.5

Two-Step RT-PCR SuperMix (15 kb)
abx098026-1ml	Abbexa	1 ml	Ask for price

Two-Step RT-PCR SuperMix (15 kb)
abx098026-200l	Abbexa	200 µl	Ask for price

All-in-One cDNA Synthesis SuperMix
B24403	Bimake	200 ractions	EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408	Bimake	1000 ractions	EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

OmniPCR HS Supermix w Fluorescent dye
MBA03-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X)
MB202-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

OmniPCR FAST Supermix w Fluorescent dye
MBA02-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

OmniPCR Long Supermix w Fluorescent dye
MBA04-0100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 16

OnePCR Ultra Supermix w Fluorescent Dye
MBS402026-100Reactions	MyBiosource	100Reactions	EUR 180

OnePCR Ultra Supermix w Fluorescent Dye
MBS402026-4Reactions	MyBiosource	4Reactions	EUR 160

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix
abx098858-100l	Abbexa	100 µl	EUR 850

Fly First-Strand cDNA Synthesis SuperMix
abx098858-1ml	Abbexa	1 ml	Ask for price

Fly First-Strand cDNA Synthesis SuperMix
abx098858-200l	Abbexa	200 µl	EUR 1037.5

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 1028.4

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-100l	Abbexa	100 µl	EUR 875

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-100l	Abbexa	100 µl	EUR 950

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200l	Abbexa	200 µl	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-100l	Abbexa	100 µl	EUR 1212.5

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-1ml	Abbexa	1 ml	Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200l	Abbexa	200 µl	Ask for price

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems	Abbexa	20 rxns × 20 ul Systems	EUR 878.4

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-100l	Abbexa	100 µl	EUR 950

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-1ml	Abbexa	1 ml	Ask for price

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-200l	Abbexa	200 µl	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 1062

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-100l	Abbexa	100 µl	EUR 1212.5

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-1ml	Abbexa	1 ml	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-200l	Abbexa	200 µl	Ask for price

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
20-abx098007	Abbexa	Ask for price Ask for price	1 ml 5 ml

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix (GC Rich)
20-abx09804120ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
abx098007-100l	Abbexa	100 µl	EUR 262.5

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)
abx098007-200l	Abbexa	200 µl	EUR 475

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-100l	Abbexa	100 µl	EUR 1125

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-200l	Abbexa	200 µl	Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-100l	Abbexa	100 µl	EUR 450

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-200l	Abbexa	200 µl	EUR 1000

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-100l	Abbexa	100 µl	EUR 737.5

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-1ml	Abbexa	1 ml	Ask for price

Probe One-Step qRT-PCR SuperMix (GC Rich)
abx098041-200l	Abbexa	200 µl	EUR 1050

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 693.6

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-100l	Abbexa	100 µl	EUR 850

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-1ml	Abbexa	1 ml	Ask for price

First-Strand cDNA Synthesis SuperMix for PCR
abx098018-200l	Abbexa	200 µl	Ask for price

Different options of PrimerMapper embrace allele-specific design options for SNP genotyping, a distant BLAST window to NCBI databases, and distant sequence retrieval from GenBank and dbSNP. PrimerMapper is hosted at GitHub and freely out there with out restriction.