Data on PCR primer design for glucose 6-phosphate dehydrogenase gene and the effects of dietary carbohydrate levels on its expression in the liver of Malaysian mahseer.

Data on PCR primer design for glucose 6-phosphate dehydrogenase gene and the effects of dietary carbohydrate levels on its expression in the liver of Malaysian mahseer.

The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the metabolite glucose-6-phosphate in producing NADPH throughout the first part of pentose-phosphate pathway thus gives decreasing energy to all cells for mobile development, antioxidant defence, and biosynthetic reactions in all dwelling organism.

The deliberate inclusion of starch as carbohydrate supply in business feed nonetheless might have an effect on the G6PD hepatic exercise in cultured fish. We designed a set of primers to focus on G6PD gene within the well-liked Malaysian aquaculture species, Tor tambroides. For this dataset, the molecular traits of obtained T. tambroides G6PD (TtG6PD) nucleotide sequence was analysed utilizing a number of alignments and phylogenetic analyses of the deduced amino acids. The set of primers obtained had been then utilized in a examine to judge the impact of various dietary carbohydrate inclusion ranges on the hepatic TtG6PD mRNA expression of the T. tambroides fingerlings.

4 teams of fish got a dietary remedy of 15%, 20%, 25% and 30% starch on the optimum inclusion stage of 23.4% for 10 weeks. The TtG6PD mRNA transcripts had been measured utilizing real-time-PCR assays and its expression normalized towards β-actin, which acts as the inner management gene.

This text gives supportive information in relation between hepatic TtG6PD mRNA gene expression in T. tambroides and the way it’s influenced by its dietary carbohydrate consumption. These information can even help in additional dietary genomic research of carbohydrate and power utilization for all species within the mahseer household.

Primer design for quantitative real-time PCR for the rising Coronavirus SARS-CoV-2

In December 2019, a brand new coronavirus illness (COVID-19) outbreak occurred in Wuhan, China. Extreme acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus identified to contaminate people, is extremely contagious and has quickly expanded worldwide since its discovery.

Quantitative nucleic acid testing has turn out to be the gold normal for analysis and guiding scientific selections concerning the usage of antiviral remedy. Nonetheless, the RT-qPCR assays concentrating on SARS-CoV-2 have various challenges, particularly by way of primer design. Primers are the pivotal elements of a RT-qPCR assay. As soon as virus mutation and recombination happen, it’s tough to successfully diagnose viral an infection by current RT-qPCR primers. Some primers and probes have additionally been made obtainable on the WHO web site for reference.

Data on PCR primer design for glucose 6-phosphate dehydrogenase gene and the effects of dietary carbohydrate levels on its expression in the liver of Malaysian mahseer.

Nonetheless, no earlier assessment has systematically in contrast the beforehand reported primers and probes and described tips on how to design new primers within the occasion of a brand new coronavirus an infection.

This assessment focuses on how primers and probes could be designed methodically and rationally, and the way the sensitivity and specificity of the detection course of could be improved. This transient assessment shall be helpful for the correct analysis and well timed remedy of the brand new coronavirus pneumonia.

EasyPrimer: user-friendly instrument for pan-PCR/HRM primers design. Improvement of an HRM protocol on wzi gene for quick Klebsiella pneumoniae typing.

On this work we current EasyPrimer, a user-friendly on-line instrument developed to help pan-PCR and Excessive Decision Melting (HRM) primer design. The instrument finds essentially the most appropriate areas for primer design in a gene alignment and returns a transparent graphical illustration of their positions on the consensus sequence.

EasyPrimer is especially helpful in tough contexts, e.g. on gene alignments of tons of of sequences and/or on extremely variable genes. HRM evaluation is an rising methodology for quick and value saving bacterial typing and an HRM scheme of six primer pairs on 5 Multi-Locus Sequence Kind (MLST) genes is already obtainable for Klebsiella pneumoniae.

We validated the instrument designing a scheme of two HRM primer pairs on the hypervariable gene wzi of Klebsiella pneumoniae and in contrast the 2 schemes. The wzi scheme resulted to have a discriminatory energy akin to the HRM MLST scheme, utilizing just one third of primer pairs.

Then we efficiently used the wzi HRM primer scheme to reconstruct a Klebsiella pneumoniae nosocomial outbreak in few hours. The usage of hypervariable genes reduces the variety of HRM primer pairs required for bacterial typing permitting to carry out price saving, large-scale surveillance applications.

ampPD: An Automated PrimerDesign Device for Extremely Multiplexed, Single-Tube Tiled Amplicon PCR for Resequencing of Tumor Samples.

PCR-based goal enrichment is extensively used to arrange libraries for subsequent technology sequencing (NGS). Amplicon tiling is commonly used for assay design however may end up in amplicon overlap that reduces assay sensitivity. Pillar Biosciences™ SLIMamp® enrichment expertise permits environment friendly amplicon tiling even with overlapping amplicons. Right here, we current an automatic primer design instrument (ampPD) for amplicon tiling and consider the efficiency of an assay designed towards the TP53 tumor suppressor gene. The ampPD workflow contains goal preparation, candidate primer technology, primer choice and pooling.

All 11 TP53 coding exons had been focused for amplification and efficiency of the assay design was evaluated by synthesizing and pooling the ampPD output primers to arrange SLIMamp libraries from 10ng, 5ng and 1ng enter DNA from FFPE tumor samples. Ensuing libraries had been normalized, pooled and sequenced on an Illumina MiSeq. Automated TP53 primer design generated an preliminary pool of 627 suitable primers that was diminished to an optimized pool of 19 amplicons, of which 16 amplicons had been overlapping. The whole design took roughly 10 seconds to finish.

The assay displayed excessive protection uniformity, with 100% of focused based mostly coated >0.2x imply protection for all samples. For the 5ng and 10ng samples, variant detection was extremely reproducible and 100% concordant with identified end result and a median 86% on-target fee. For the 1ng samples, all constructive variants had been detected however with elevated background noise and a decrease median on-target fee. The ampPD instrument is a fast and sturdy primer design pipeline for amplicon tiling assays. Utilizing TP53 as a mannequin, the ensuing primers supplied uniform protection and delicate variant detection at FFPE-derived DNA inputs >1ng.

SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

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Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

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SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

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Description: SYBR Green qPCR Master Mix (Low ROX)

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HY-K0522 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

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Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix

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Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns

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Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns

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Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns

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Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns

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Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns

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BioEasy Master Mix (SYBR Green)

TRI-B25L1 200T
EUR 403.2

SYBR Green cRNA PCR Master Mix

SGM002 2 ml
EUR 174

EnoGene Green qPCR Master Mix

E31M001 500 reaction
EUR 75

ChamQ SYBR qPCR Master Mix

Q311-00-100rxns 100 rxns
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ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix

Q311-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix

Q311-03-2500rxns 2,500 rxns
EUR 562.79

Apex qPCR GREEN Master Mix

42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix

42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix

42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix

42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix

42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

BioEasy Master Mix (SYBR Green, No ROX)

TRI-B25L1D 200T
EUR 403.2

BioEasy Master Mix (SYBR Green, low ROX)

TRI-B25L1C 200T
EUR 403.2

BioEasy Master Mix (SYBR Green, high ROX)

TRI-B25L1B 200T
EUR 403.2

ChamQ SYBR Color qPCR Master Mix

Q411-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix

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EUR 145.62

ChamQ SYBR Color qPCR Master Mix

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ChamQ SYBR Color qPCR Master Mix

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EUR 656.51

AceQ Universal SYBR qPCR Master Mix

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EUR 1.09

AceQ Universal SYBR qPCR Master Mix

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EUR 152.72

AceQ Universal SYBR qPCR Master Mix

Q511-02-500rxns 500 rxns
EUR 158.14

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 688.5

AceQ Universal SYBR qPCR Master Mix

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EUR 712.94

miRNA Universal SYBR qPCR Master Mix

MQ101-00-100rxns 100 rxns
EUR 1.09

miRNA Universal SYBR qPCR Master Mix

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EUR 38.9

miRNA Universal SYBR qPCR Master Mix

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EUR 40.28

miRNA Universal SYBR qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 145

miRNA Universal SYBR qPCR Master Mix

MQ101-02-500rxns 500 rxns
EUR 150.15

ChamQ Universal SYBR qPCR Master Mix

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EUR 1.09

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 152.72

ChamQ Universal SYBR qPCR Master Mix

Q711-02-500rxns 500 rxns
EUR 158.14

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 688.5

ChamQ Universal SYBR qPCR Master Mix

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EUR 712.94

HotStart™ 2X Green qPCR Master Mix

K1070-25 25ml
EUR 392
Description: Reverse Transcription & PCR|qPCR

HotStart™ 2X Green qPCR Master Mix

K1070-5 5ml
EUR 124
Description: Reverse Transcription & PCR|qPCR

HotStart™ 2X Green qPCR Master Mix

K1070-50 50ml
EUR 768
Description: Reverse Transcription & PCR|qPCR

2×SYBR Green qPCR Mix

E37F021-10x5mL 10×5mL
EUR 1360
Description: DsDNA, ssDNA, RNA quantitative detection kit

2×SYBR Green qPCR Mix

E37F021-5mL 5mL
EUR 227
Description: Fluorescent quantitative PCR kits

2×SYBR Green qPCR Mix

MBS8579840-200Tests 200Tests
EUR 285

2×SYBR Green qPCR Mix

MBS8579840-50Tests 50Tests
EUR 180

2×SYBR Green qPCR Mix

MBS8579840-5x200Tests 5x200Tests
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2×SYBR Green qPCR Mix

MBS8579840-5x50Tests 5x50Tests
EUR 770

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03-2500rxns 2,500 rxns
EUR 562.79

Taq Pro Universal SYBR qPCR Master Mix

Q712-00-100rxns 100 rxns
EUR 1.09

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 161

Taq Pro Universal SYBR qPCR Master Mix

Q712-02-500rxns 500 rxns
EUR 166.72

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 725

Taq Pro Universal SYBR qPCR Master Mix

Q712-03-2500rxns 2,500 rxns
EUR 750.74

miRNA SYBR Green qPCR Mix

4308A 100 Tests*20μL
EUR 80

miRNA SYBR Green qPCR Mix

4308B 200 Tests*20μL
EUR 150

miRNA SYBR Green qPCR Mix

4308C 500 Tests*20μL
EUR 300

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns

QLMM10 each Ask for price

HotStart™ 2X FAST Green qPCR Master Mix

K1171-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X Green qPCR Master Mix

K1170-25 25ml
EUR 392
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X Green qPCR Master Mix

K1170-5 5ml
EUR 124
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X Green qPCR Master Mix

K1170-50 50ml
EUR 752
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03-2500rxns 2,500 rxns
EUR 562.79

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03-2500rxns 2,500 rxns
EUR 562.79

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03-2500rxns 2,500 rxns
EUR 656.51

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns

QLMM12 each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns

QLMM17 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns

QLMM02 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns

QLMM07 each Ask for price

Hieff® miRNA Universal qPCR SYBR Master Mix

11171ES03 1 mL
EUR 35

Hieff® miRNA Universal qPCR SYBR Master Mix

11171ES08 5 mL
EUR 150

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03-2500rxns 2,500 rxns
EUR 656.51

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02-500rxns 500 rxns
EUR 145.62

Future work will prolong the design pipeline to bigger and extra advanced panels, enabling fast creation of single-tube focused sequencing assays for quite a lot of functions.

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