The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the metabolite glucose-6-phosphate in producing NADPH throughout the first part of pentose-phosphate pathway thus gives decreasing energy to all cells for mobile development, antioxidant defence, and biosynthetic reactions in all dwelling organism.
The deliberate inclusion of starch as carbohydrate supply in business feed nonetheless might have an effect on the G6PD hepatic exercise in cultured fish. We designed a set of primers to focus on G6PD gene within the well-liked Malaysian aquaculture species, Tor tambroides. For this dataset, the molecular traits of obtained T. tambroides G6PD (TtG6PD) nucleotide sequence was analysed utilizing a number of alignments and phylogenetic analyses of the deduced amino acids. The set of primers obtained had been then utilized in a examine to judge the impact of various dietary carbohydrate inclusion ranges on the hepatic TtG6PD mRNA expression of the T. tambroides fingerlings.
4 teams of fish got a dietary remedy of 15%, 20%, 25% and 30% starch on the optimum inclusion stage of 23.4% for 10 weeks. The TtG6PD mRNA transcripts had been measured utilizing real-time-PCR assays and its expression normalized towards β-actin, which acts as the inner management gene.
This text gives supportive information in relation between hepatic TtG6PD mRNA gene expression in T. tambroides and the way it’s influenced by its dietary carbohydrate consumption. These information can even help in additional dietary genomic research of carbohydrate and power utilization for all species within the mahseer household.
Primer design for quantitative real-time PCR for the rising Coronavirus SARS-CoV-2
In December 2019, a brand new coronavirus illness (COVID-19) outbreak occurred in Wuhan, China. Extreme acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus identified to contaminate people, is extremely contagious and has quickly expanded worldwide since its discovery.
Quantitative nucleic acid testing has turn out to be the gold normal for analysis and guiding scientific selections concerning the usage of antiviral remedy. Nonetheless, the RT-qPCR assays concentrating on SARS-CoV-2 have various challenges, particularly by way of primer design. Primers are the pivotal elements of a RT-qPCR assay. As soon as virus mutation and recombination happen, it’s tough to successfully diagnose viral an infection by current RT-qPCR primers. Some primers and probes have additionally been made obtainable on the WHO web site for reference.
Nonetheless, no earlier assessment has systematically in contrast the beforehand reported primers and probes and described tips on how to design new primers within the occasion of a brand new coronavirus an infection.
This assessment focuses on how primers and probes could be designed methodically and rationally, and the way the sensitivity and specificity of the detection course of could be improved. This transient assessment shall be helpful for the correct analysis and well timed remedy of the brand new coronavirus pneumonia.
EasyPrimer: user-friendly instrument for pan-PCR/HRM primers design. Improvement of an HRM protocol on wzi gene for quick Klebsiella pneumoniae typing.
On this work we current EasyPrimer, a user-friendly on-line instrument developed to help pan-PCR and Excessive Decision Melting (HRM) primer design. The instrument finds essentially the most appropriate areas for primer design in a gene alignment and returns a transparent graphical illustration of their positions on the consensus sequence.
EasyPrimer is especially helpful in tough contexts, e.g. on gene alignments of tons of of sequences and/or on extremely variable genes. HRM evaluation is an rising methodology for quick and value saving bacterial typing and an HRM scheme of six primer pairs on 5 Multi-Locus Sequence Kind (MLST) genes is already obtainable for Klebsiella pneumoniae.
We validated the instrument designing a scheme of two HRM primer pairs on the hypervariable gene wzi of Klebsiella pneumoniae and in contrast the 2 schemes. The wzi scheme resulted to have a discriminatory energy akin to the HRM MLST scheme, utilizing just one third of primer pairs.
Then we efficiently used the wzi HRM primer scheme to reconstruct a Klebsiella pneumoniae nosocomial outbreak in few hours. The usage of hypervariable genes reduces the variety of HRM primer pairs required for bacterial typing permitting to carry out price saving, large-scale surveillance applications.
ampPD: An Automated PrimerDesign Device for Extremely Multiplexed, Single-Tube Tiled Amplicon PCR for Resequencing of Tumor Samples.
PCR-based goal enrichment is extensively used to arrange libraries for subsequent technology sequencing (NGS). Amplicon tiling is commonly used for assay design however may end up in amplicon overlap that reduces assay sensitivity. Pillar Biosciences™ SLIMamp® enrichment expertise permits environment friendly amplicon tiling even with overlapping amplicons. Right here, we current an automatic primer design instrument (ampPD) for amplicon tiling and consider the efficiency of an assay designed towards the TP53 tumor suppressor gene. The ampPD workflow contains goal preparation, candidate primer technology, primer choice and pooling.
All 11 TP53 coding exons had been focused for amplification and efficiency of the assay design was evaluated by synthesizing and pooling the ampPD output primers to arrange SLIMamp libraries from 10ng, 5ng and 1ng enter DNA from FFPE tumor samples. Ensuing libraries had been normalized, pooled and sequenced on an Illumina MiSeq. Automated TP53 primer design generated an preliminary pool of 627 suitable primers that was diminished to an optimized pool of 19 amplicons, of which 16 amplicons had been overlapping. The whole design took roughly 10 seconds to finish.
The assay displayed excessive protection uniformity, with 100% of focused based mostly coated >0.2x imply protection for all samples. For the 5ng and 10ng samples, variant detection was extremely reproducible and 100% concordant with identified end result and a median 86% on-target fee. For the 1ng samples, all constructive variants had been detected however with elevated background noise and a decrease median on-target fee. The ampPD instrument is a fast and sturdy primer design pipeline for amplicon tiling assays. Utilizing TP53 as a mannequin, the ensuing primers supplied uniform protection and delicate variant detection at FFPE-derived DNA inputs >1ng.
) SYBR Green qPCR Master Mix (No ROX) |
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C0006-1mg |
TargetMol Chemicals |
1mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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C0006-50mg |
TargetMol Chemicals |
50mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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C0006-5mg |
TargetMol Chemicals |
5mg |
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Description: SYBR Green qPCR Master Mix (No ROX) |
SYBR Green qPCR Master Mix (No ROX) |
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HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
) SYBR Green qPCR Master Mix (Low ROX) |
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C0008-10mg |
TargetMol Chemicals |
10mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-1g |
TargetMol Chemicals |
1g |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-1mg |
TargetMol Chemicals |
1mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-50mg |
TargetMol Chemicals |
50mg |
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Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
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C0008-5mg |
TargetMol Chemicals |
5mg |
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|
Description: SYBR Green qPCR Master Mix (Low ROX) |
SYBR Green qPCR Master Mix (Low ROX) |
|
HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
) SYBR Green qPCR Master Mix (High ROX) |
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C0007-10mg |
TargetMol Chemicals |
10mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-1g |
TargetMol Chemicals |
1g |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-1mg |
TargetMol Chemicals |
1mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-50mg |
TargetMol Chemicals |
50mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
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C0007-5mg |
TargetMol Chemicals |
5mg |
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Description: SYBR Green qPCR Master Mix (High ROX) |
SYBR Green qPCR Master Mix (High ROX) |
|
HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 135.6 |
) 2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21702 |
Bimake |
5 ml |
EUR 268.8 |
|
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
|
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) 2x SYBR Green qPCR Master Mix (High ROX) |
|
B21402 |
Bimake |
5 ml |
EUR 268.8 |
|
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
|
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
|
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
 AceQ qPCR SYBR® Green Master Mix |
|
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
|
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
 Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns |
|
THK0221 |
Westburg |
each |
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 Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns |
|
THK0222 |
Westburg |
each |
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 Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns |
|
THK0223 |
Westburg |
each |
EUR 3324.5 |
 Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns |
|
THK0241 |
Westburg |
each |
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 Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns |
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THK0242 |
Westburg |
each |
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 Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns |
|
THK0243 |
Westburg |
each |
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 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns |
|
THK0251 |
Westburg |
each |
EUR 232.17 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns |
|
THK0252 |
Westburg |
each |
EUR 1046.4 |
 Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns |
|
THK0253 |
Westburg |
each |
Ask for price |
) BioEasy Master Mix (SYBR Green) |
|
TRI-B25L1 |
TRI Biotech |
200T |
EUR 403.2 |
 SYBR Green cRNA PCR Master Mix |
|
SGM002 |
Signosis |
2 ml |
EUR 174 |
 EnoGene Green qPCR Master Mix |
|
E31M001 |
EnoGene |
500 reaction |
EUR 75 |
 ChamQ SYBR qPCR Master Mix |
|
Q311-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ SYBR qPCR Master Mix |
|
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix |
|
Q311-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix |
|
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix |
|
Q311-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
 Apex qPCR GREEN Master Mix |
|
42-116PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
|
Description: 2X, Without ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-117PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
|
Description: 2X, Without ROX, 50ml |
Apex qPCR GREEN Master Mix |
|
42-118PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 338.88 |
|
Description: 2X, Low ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-119PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 2926.74 |
|
Description: 2X, Low ROX, 50ml |
Apex qPCR GREEN Master Mix |
|
42-120PG |
Genesee Scientific |
400 x 25µl Reactions/Unit |
EUR 341.44 |
|
Description: 2X, High ROX, 5ml |
Apex qPCR GREEN Master Mix |
|
42-121PG |
Genesee Scientific |
4000 x 25µl Reactions/Unit |
EUR 3095.57 |
|
Description: 2X, High ROX, 50ml |
) BioEasy Master Mix (SYBR Green, No ROX) |
|
TRI-B25L1D |
TRI Biotech |
200T |
EUR 403.2 |
) BioEasy Master Mix (SYBR Green, low ROX) |
|
TRI-B25L1C |
TRI Biotech |
200T |
EUR 403.2 |
) BioEasy Master Mix (SYBR Green, high ROX) |
|
TRI-B25L1B |
TRI Biotech |
200T |
EUR 403.2 |
 ChamQ SYBR Color qPCR Master Mix |
|
Q411-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 AceQ Universal SYBR qPCR Master Mix |
|
Q511-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-02-500rxns |
Vazyme |
500 rxns |
EUR 158.14 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 712.94 |
 miRNA Universal SYBR qPCR Master Mix |
|
MQ101-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 38.9 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-01-125rxns |
Vazyme |
125 rxns |
EUR 40.28 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 145 |
miRNA Universal SYBR qPCR Master Mix |
|
MQ101-02-500rxns |
Vazyme |
500 rxns |
EUR 150.15 |
 ChamQ Universal SYBR qPCR Master Mix |
|
Q711-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 152.72 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02-500rxns |
Vazyme |
500 rxns |
EUR 158.14 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 688.5 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 712.94 |
 HotStart™ 2X Green qPCR Master Mix |
|
K1070-25 |
ApexBio |
25ml |
EUR 392 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
HotStart™ 2X Green qPCR Master Mix |
|
K1070-5 |
ApexBio |
5ml |
EUR 124 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
HotStart™ 2X Green qPCR Master Mix |
|
K1070-50 |
ApexBio |
50ml |
EUR 768 |
|
|
|
Description: Reverse Transcription & PCR|qPCR |
 2×SYBR Green qPCR Mix |
|
E37F021-10x5mL |
EnoGene |
10×5mL |
EUR 1360 |
|
Description: DsDNA, ssDNA, RNA quantitative detection kit |
2×SYBR Green qPCR Mix |
|
E37F021-5mL |
EnoGene |
5mL |
EUR 227 |
|
Description: Fluorescent quantitative PCR kits |
2×SYBR Green qPCR Mix |
|
MBS8579840-200Tests |
MyBiosource |
200Tests |
EUR 285 |
2×SYBR Green qPCR Mix |
|
MBS8579840-50Tests |
MyBiosource |
50Tests |
EUR 180 |
2×SYBR Green qPCR Mix |
|
MBS8579840-5x200Tests |
MyBiosource |
5x200Tests |
EUR 1230 |
2×SYBR Green qPCR Mix |
|
MBS8579840-5x50Tests |
MyBiosource |
5x50Tests |
EUR 770 |
) ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
 Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-00-100rxns |
Vazyme |
100 rxns |
EUR 1.09 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 161 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-02-500rxns |
Vazyme |
500 rxns |
EUR 166.72 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 725 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 750.74 |
 miRNA SYBR Green qPCR Mix |
|
4308A |
Unibiotest |
100 Tests*20μL |
EUR 80 |
miRNA SYBR Green qPCR Mix |
|
4308B |
Unibiotest |
200 Tests*20μL |
EUR 150 |
miRNA SYBR Green qPCR Mix |
|
4308C |
Unibiotest |
500 Tests*20μL |
EUR 300 |
, 100rxns) ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns |
|
QLMM10 |
Vivantis |
each |
Ask for price |
 HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-25 |
ApexBio |
25ml |
EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-5 |
ApexBio |
5ml |
EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ 2X FAST Green qPCR Master Mix |
|
K1171-50 |
ApexBio |
50ml |
EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
 HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-25 |
ApexBio |
25ml |
EUR 392 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-5 |
ApexBio |
5ml |
EUR 124 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X Green qPCR Master Mix |
|
K1170-50 |
ApexBio |
50ml |
EUR 752 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
) ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
) ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02-500rxns |
Vazyme |
500 rxns |
EUR 124.83 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 562.79 |
) ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-25 |
ApexBio |
25ml |
EUR 424 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-5 |
ApexBio |
5ml |
EUR 132 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
HotStart™ Universal 2X FAST Green qPCR Master Mix |
|
K1172-50 |
ApexBio |
50ml |
EUR 826.4 |
|
Description: Reverse Transcription & PCR|qPCR/Real-time PCR |
 ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
|
QLMM12 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns |
|
QLMM17 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns |
|
QLMM02 |
Vivantis |
each |
Ask for price |
 ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns |
|
QLMM07 |
Vivantis |
each |
Ask for price |
) ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271 |
AAT Bioquest |
5 mL |
EUR 508 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
) ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
Future work will prolong the design pipeline to bigger and extra advanced panels, enabling fast creation of single-tube focused sequencing assays for quite a lot of functions.
