Data on PCR primer design for glucose 6-phosphate dehydrogenase gene and the effects of dietary carbohydrate levels on its expression in the liver of Malaysian mahseer.

The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the metabolite glucose-6-phosphate in producing NADPH throughout the first part of pentose-phosphate pathway thus gives decreasing energy to all cells for mobile development, antioxidant defence, and biosynthetic reactions in all dwelling organism.

The deliberate inclusion of starch as carbohydrate supply in business feed nonetheless might have an effect on the G6PD hepatic exercise in cultured fish. We designed a set of primers to focus on G6PD gene within the well-liked Malaysian aquaculture species, Tor tambroides. For this dataset, the molecular traits of obtained T. tambroides G6PD (TtG6PD) nucleotide sequence was analysed utilizing a number of alignments and phylogenetic analyses of the deduced amino acids. The set of primers obtained had been then utilized in a examine to judge the impact of various dietary carbohydrate inclusion ranges on the hepatic TtG6PD mRNA expression of the T. tambroides fingerlings.

4 teams of fish got a dietary remedy of 15%, 20%, 25% and 30% starch on the optimum inclusion stage of 23.4% for 10 weeks. The TtG6PD mRNA transcripts had been measured utilizing real-time-PCR assays and its expression normalized towards β-actin, which acts as the inner management gene.

This text gives supportive information in relation between hepatic TtG6PD mRNA gene expression in T. tambroides and the way it’s influenced by its dietary carbohydrate consumption. These information can even help in additional dietary genomic research of carbohydrate and power utilization for all species within the mahseer household.

Primer design for quantitative real-time PCR for the rising Coronavirus SARS-CoV-2

In December 2019, a brand new coronavirus illness (COVID-19) outbreak occurred in Wuhan, China. Extreme acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus identified to contaminate people, is extremely contagious and has quickly expanded worldwide since its discovery.

Quantitative nucleic acid testing has turn out to be the gold normal for analysis and guiding scientific selections concerning the usage of antiviral remedy. Nonetheless, the RT-qPCR assays concentrating on SARS-CoV-2 have various challenges, particularly by way of primer design. Primers are the pivotal elements of a RT-qPCR assay. As soon as virus mutation and recombination happen, it’s tough to successfully diagnose viral an infection by current RT-qPCR primers. Some primers and probes have additionally been made obtainable on the WHO web site for reference.

Nonetheless, no earlier assessment has systematically in contrast the beforehand reported primers and probes and described tips on how to design new primers within the occasion of a brand new coronavirus an infection.

This assessment focuses on how primers and probes could be designed methodically and rationally, and the way the sensitivity and specificity of the detection course of could be improved. This transient assessment shall be helpful for the correct analysis and well timed remedy of the brand new coronavirus pneumonia.

EasyPrimer: user-friendly instrument for pan-PCR/HRM primers design. Improvement of an HRM protocol on wzi gene for quick Klebsiella pneumoniae typing.

On this work we current EasyPrimer, a user-friendly on-line instrument developed to help pan-PCR and Excessive Decision Melting (HRM) primer design. The instrument finds essentially the most appropriate areas for primer design in a gene alignment and returns a transparent graphical illustration of their positions on the consensus sequence.

EasyPrimer is especially helpful in tough contexts, e.g. on gene alignments of tons of of sequences and/or on extremely variable genes. HRM evaluation is an rising methodology for quick and value saving bacterial typing and an HRM scheme of six primer pairs on 5 Multi-Locus Sequence Kind (MLST) genes is already obtainable for Klebsiella pneumoniae.

We validated the instrument designing a scheme of two HRM primer pairs on the hypervariable gene wzi of Klebsiella pneumoniae and in contrast the 2 schemes. The wzi scheme resulted to have a discriminatory energy akin to the HRM MLST scheme, utilizing just one third of primer pairs.

Then we efficiently used the wzi HRM primer scheme to reconstruct a Klebsiella pneumoniae nosocomial outbreak in few hours. The usage of hypervariable genes reduces the variety of HRM primer pairs required for bacterial typing permitting to carry out price saving, large-scale surveillance applications.

PCR-based goal enrichment is extensively used to arrange libraries for subsequent technology sequencing (NGS). Amplicon tiling is commonly used for assay design however may end up in amplicon overlap that reduces assay sensitivity. Pillar Biosciences™ SLIMamp® enrichment expertise permits environment friendly amplicon tiling even with overlapping amplicons. Right here, we current an automatic primer design instrument (ampPD) for amplicon tiling and consider the efficiency of an assay designed towards the TP53 tumor suppressor gene. The ampPD workflow contains goal preparation, candidate primer technology, primer choice and pooling.

All 11 TP53 coding exons had been focused for amplification and efficiency of the assay design was evaluated by synthesizing and pooling the ampPD output primers to arrange SLIMamp libraries from 10ng, 5ng and 1ng enter DNA from FFPE tumor samples. Ensuing libraries had been normalized, pooled and sequenced on an Illumina MiSeq. Automated TP53 primer design generated an preliminary pool of 627 suitable primers that was diminished to an optimized pool of 19 amplicons, of which 16 amplicons had been overlapping. The whole design took roughly 10 seconds to finish.

The assay displayed excessive protection uniformity, with 100% of focused based mostly coated >0.2x imply protection for all samples. For the 5ng and 10ng samples, variant detection was extremely reproducible and 100% concordant with identified end result and a median 86% on-target fee. For the 1ng samples, all constructive variants had been detected however with elevated background noise and a decrease median on-target fee. The ampPD instrument is a fast and sturdy primer design pipeline for amplicon tiling assays. Utilizing TP53 as a mannequin, the ensuing primers supplied uniform protection and delicate variant detection at FFPE-derived DNA inputs >1ng.

SYBR Green qPCR Master Mix (No ROX)
C0006-50mg	TargetMol Chemicals	50mg	Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)
C0006-5mg	TargetMol Chemicals	5mg	Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)
HY-K0523	MedChemExpress	5 mL (500 rxns )	EUR 308.4

SYBR Green qPCR Master Mix (Low ROX)
C0008-10mg	TargetMol Chemicals	10mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-1g	TargetMol Chemicals	1g	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-1mg	TargetMol Chemicals	1mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-50mg	TargetMol Chemicals	50mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-5mg	TargetMol Chemicals	5mg	Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522	MedChemExpress	5 mL (500 rxns )	EUR 308.4

SYBR Green qPCR Master Mix (High ROX)
C0007-10mg	TargetMol Chemicals	10mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-1g	TargetMol Chemicals	1g	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-1mg	TargetMol Chemicals	1mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-50mg	TargetMol Chemicals	50mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-5mg	TargetMol Chemicals	5mg	Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
HY-K0521	MedChemExpress	1 mL (100 rxns)	EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)
B21702	Bimake	5 ml	EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
B21703	Bimake	25 ml	EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21402	Bimake	5 ml	EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)
B21403	Bimake	25 ml	EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix
Q111-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR SYBR® Green Master Mix
Q111-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-25	ApexBio	25ml	EUR 336

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-5	ApexBio	5ml	EUR 96

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-50	ApexBio	50ml	EUR 672

ig SYBR Green qPCR 2X Master Mix - 200 Reactions
3354	Intact Genomics	200	EUR 110
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-25	ApexBio	25ml	EUR 336
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-5	ApexBio	5ml	EUR 96
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-50	ApexBio	50ml	EUR 672
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns
THK0221	Westburg	each	Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns
THK0222	Westburg	each	Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns
THK0223	Westburg	each	EUR 3324.5

Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns
THK0241	Westburg	each	Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns
THK0242	Westburg	each	Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns
THK0243	Westburg	each	Ask for price

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns
THK0251	Westburg	each	EUR 232.17

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns
THK0252	Westburg	each	EUR 1046.4

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns
THK0253	Westburg	each	Ask for price

BioEasy Master Mix (SYBR Green)
TRI-B25L1	TRI Biotech	200T	EUR 403.2

ChamQ SYBR qPCR Master Mix
Q311-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix
Q311-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

ChamQ SYBR qPCR Master Mix
Q311-00-100rxns	Vazyme	100 rxns	EUR 1.09

ChamQ SYBR qPCR Master Mix
Q311-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix
Q311-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

Apex qPCR GREEN Master Mix
42-116PG	Genesee Scientific	400 x 25µl Reactions/Unit	EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix
42-117PG	Genesee Scientific	4000 x 25µl Reactions/Unit	EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix
42-118PG	Genesee Scientific	400 x 25µl Reactions/Unit	EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix
42-119PG	Genesee Scientific	4000 x 25µl Reactions/Unit	EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix
42-120PG	Genesee Scientific	400 x 25µl Reactions/Unit	EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix
42-121PG	Genesee Scientific	4000 x 25µl Reactions/Unit	EUR 3095.57
Description: 2X, High ROX, 50ml

BioEasy Master Mix (SYBR Green, No ROX)
TRI-B25L1D	TRI Biotech	200T	EUR 403.2

BioEasy Master Mix (SYBR Green, low ROX)
TRI-B25L1C	TRI Biotech	200T	EUR 403.2

BioEasy Master Mix (SYBR Green, high ROX)
TRI-B25L1B	TRI Biotech	200T	EUR 403.2

ChamQ SYBR Color qPCR Master Mix
Q411-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix
Q411-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR Color qPCR Master Mix
Q411-00-100rxns	Vazyme	100 rxns	EUR 1.09

ChamQ SYBR Color qPCR Master Mix
Q411-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix
Q411-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

AceQ Universal SYBR qPCR Master Mix
Q511-02	Vazyme	500 rxn (20 μl/rxn)	EUR 152.72

AceQ Universal SYBR qPCR Master Mix
Q511-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 688.5

AceQ Universal SYBR qPCR Master Mix
Q511-00-100rxns	Vazyme	100 rxns	EUR 1.09

AceQ Universal SYBR qPCR Master Mix
Q511-02-500rxns	Vazyme	500 rxns	EUR 158.14

AceQ Universal SYBR qPCR Master Mix
Q511-03-2500rxns	Vazyme	2,500 rxns	EUR 712.94

miRNA Universal SYBR qPCR Master Mix
MQ101-01	Vazyme	125 rxn(20 μl/rxn)	EUR 38.9

miRNA Universal SYBR qPCR Master Mix
MQ101-02	Vazyme	500 rxn(20 μl/rxn)	EUR 145

ChamQ Universal SYBR qPCR Master Mix
Q711-02	Vazyme	500 rxn (20 μl/rxn)	EUR 152.72

ChamQ Universal SYBR qPCR Master Mix
Q711-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 688.5

miRNA Universal SYBR qPCR Master Mix
MQ101-00-100rxns	Vazyme	100 rxns	EUR 1.09

miRNA Universal SYBR qPCR Master Mix
MQ101-01-125rxns	Vazyme	125 rxns	EUR 40.28

miRNA Universal SYBR qPCR Master Mix
MQ101-02-500rxns	Vazyme	500 rxns	EUR 150.15

ChamQ Universal SYBR qPCR Master Mix
Q711-00-100rxns	Vazyme	100 rxns	EUR 1.09

ChamQ Universal SYBR qPCR Master Mix
Q711-02-500rxns	Vazyme	500 rxns	EUR 158.14

ChamQ Universal SYBR qPCR Master Mix
Q711-03-2500rxns	Vazyme	2,500 rxns	EUR 712.94

2×SYBR Green qPCR Mix
MBS8579840-200Tests	MyBiosource	200Tests	EUR 285

2×SYBR Green qPCR Mix
MBS8579840-50Tests	MyBiosource	50Tests	EUR 180

2×SYBR Green qPCR Mix
MBS8579840-5x200Tests	MyBiosource	5x200Tests	EUR 1230

2×SYBR Green qPCR Mix
MBS8579840-5x50Tests	MyBiosource	5x50Tests	EUR 770

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

Taq Pro Universal SYBR qPCR Master Mix
Q712-02	Vazyme	500 rxns (20 μl/rxn)	EUR 161

Taq Pro Universal SYBR qPCR Master Mix
Q712-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 725

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

Taq Pro Universal SYBR qPCR Master Mix
Q712-00-100rxns	Vazyme	100 rxns	EUR 1.09

Taq Pro Universal SYBR qPCR Master Mix
Q712-02-500rxns	Vazyme	500 rxns	EUR 166.72

Taq Pro Universal SYBR qPCR Master Mix
Q712-03-2500rxns	Vazyme	2,500 rxns	EUR 750.74

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns
QLMM10	Vivantis	each	Ask for price

HotStart™ 2X FAST Green qPCR Master Mix
K1171-25	ApexBio	25ml	EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix
K1171-5	ApexBio	5ml	EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix
K1171-50	ApexBio	50ml	EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02	Vazyme	500 rxns (20 μl/rxn)	EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 543.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02-500rxns	Vazyme	500 rxns	EUR 124.83

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03-2500rxns	Vazyme	2,500 rxns	EUR 562.79

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-25	ApexBio	25ml	EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-5	ApexBio	5ml	EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-50	ApexBio	50ml	EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns
QLMM12	Vivantis	each	Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns
QLMM17	Vivantis	each	Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns
QLMM02	Vivantis	each	Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns
QLMM07	Vivantis	each	Ask for price

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02	Vazyme	500 rxns (20 μl/rxn)	EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03	Vazyme	2,500 rxns (20 μl/rxn)	EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02-500rxns	Vazyme	500 rxns	EUR 145.62

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03-2500rxns	Vazyme	2,500 rxns	EUR 656.51

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272	AAT Bioquest	1 mL	EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272-1mL	AAT Bioquest	1 mL	EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273	AAT Bioquest	5 mL	EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273-5mL	AAT Bioquest	5 mL	EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

ABsolute SYBR Green QPCR Mix 800 rxns
AB-1158/B	Westburg	each	Ask for price

ABsolute SYBR Green QPCR Mix 200 rxns
AB-1159	Westburg	each	EUR 529.74

Future work will prolong the design pipeline to bigger and extra advanced panels, enabling fast creation of single-tube focused sequencing assays for quite a lot of functions.