The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the metabolite glucose-6-phosphate in producing NADPH throughout the first part of pentose-phosphate pathway thus gives decreasing energy to all cells for mobile development, antioxidant defence, and biosynthetic reactions in all dwelling organism.
The deliberate inclusion of starch as carbohydrate supply in business feed nonetheless might have an effect on the G6PD hepatic exercise in cultured fish. We designed a set of primers to focus on G6PD gene within the well-liked Malaysian aquaculture species, Tor tambroides. For this dataset, the molecular traits of obtained T. tambroides G6PD (TtG6PD) nucleotide sequence was analysed utilizing a number of alignments and phylogenetic analyses of the deduced amino acids. The set of primers obtained had been then utilized in a examine to judge the impact of various dietary carbohydrate inclusion ranges on the hepatic TtG6PD mRNA expression of the T. tambroides fingerlings.
4 teams of fish got a dietary remedy of 15%, 20%, 25% and 30% starch on the optimum inclusion stage of 23.4% for 10 weeks. The TtG6PD mRNA transcripts had been measured utilizing real-time-PCR assays and its expression normalized towards β-actin, which acts as the inner management gene.
This text gives supportive information in relation between hepatic TtG6PD mRNA gene expression in T. tambroides and the way it’s influenced by its dietary carbohydrate consumption. These information can even help in additional dietary genomic research of carbohydrate and power utilization for all species within the mahseer household.
Primer design for quantitative real-time PCR for the rising Coronavirus SARS-CoV-2
In December 2019, a brand new coronavirus illness (COVID-19) outbreak occurred in Wuhan, China. Extreme acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus identified to contaminate people, is extremely contagious and has quickly expanded worldwide since its discovery.
Quantitative nucleic acid testing has turn out to be the gold normal for analysis and guiding scientific selections concerning the usage of antiviral remedy. Nonetheless, the RT-qPCR assays concentrating on SARS-CoV-2 have various challenges, particularly by way of primer design. Primers are the pivotal elements of a RT-qPCR assay. As soon as virus mutation and recombination happen, it’s tough to successfully diagnose viral an infection by current RT-qPCR primers. Some primers and probes have additionally been made obtainable on the WHO web site for reference.
Nonetheless, no earlier assessment has systematically in contrast the beforehand reported primers and probes and described tips on how to design new primers within the occasion of a brand new coronavirus an infection.
This assessment focuses on how primers and probes could be designed methodically and rationally, and the way the sensitivity and specificity of the detection course of could be improved. This transient assessment shall be helpful for the correct analysis and well timed remedy of the brand new coronavirus pneumonia.
EasyPrimer: user-friendly instrument for pan-PCR/HRM primers design. Improvement of an HRM protocol on wzi gene for quick Klebsiella pneumoniae typing.
On this work we current EasyPrimer, a user-friendly on-line instrument developed to help pan-PCR and Excessive Decision Melting (HRM) primer design. The instrument finds essentially the most appropriate areas for primer design in a gene alignment and returns a transparent graphical illustration of their positions on the consensus sequence.
EasyPrimer is especially helpful in tough contexts, e.g. on gene alignments of tons of of sequences and/or on extremely variable genes. HRM evaluation is an rising methodology for quick and value saving bacterial typing and an HRM scheme of six primer pairs on 5 Multi-Locus Sequence Kind (MLST) genes is already obtainable for Klebsiella pneumoniae.
We validated the instrument designing a scheme of two HRM primer pairs on the hypervariable gene wzi of Klebsiella pneumoniae and in contrast the 2 schemes. The wzi scheme resulted to have a discriminatory energy akin to the HRM MLST scheme, utilizing just one third of primer pairs.
Then we efficiently used the wzi HRM primer scheme to reconstruct a Klebsiella pneumoniae nosocomial outbreak in few hours. The usage of hypervariable genes reduces the variety of HRM primer pairs required for bacterial typing permitting to carry out price saving, large-scale surveillance applications.
ampPD: An Automated PrimerDesign Device for Extremely Multiplexed, Single-Tube Tiled Amplicon PCR for Resequencing of Tumor Samples.
PCR-based goal enrichment is extensively used to arrange libraries for subsequent technology sequencing (NGS). Amplicon tiling is commonly used for assay design however may end up in amplicon overlap that reduces assay sensitivity. Pillar Biosciences™ SLIMamp® enrichment expertise permits environment friendly amplicon tiling even with overlapping amplicons. Right here, we current an automatic primer design instrument (ampPD) for amplicon tiling and consider the efficiency of an assay designed towards the TP53 tumor suppressor gene. The ampPD workflow contains goal preparation, candidate primer technology, primer choice and pooling.
All 11 TP53 coding exons had been focused for amplification and efficiency of the assay design was evaluated by synthesizing and pooling the ampPD output primers to arrange SLIMamp libraries from 10ng, 5ng and 1ng enter DNA from FFPE tumor samples. Ensuing libraries had been normalized, pooled and sequenced on an Illumina MiSeq. Automated TP53 primer design generated an preliminary pool of 627 suitable primers that was diminished to an optimized pool of 19 amplicons, of which 16 amplicons had been overlapping. The whole design took roughly 10 seconds to finish.
The assay displayed excessive protection uniformity, with 100% of focused based mostly coated >0.2x imply protection for all samples. For the 5ng and 10ng samples, variant detection was extremely reproducible and 100% concordant with identified end result and a median 86% on-target fee. For the 1ng samples, all constructive variants had been detected however with elevated background noise and a decrease median on-target fee. The ampPD instrument is a fast and sturdy primer design pipeline for amplicon tiling assays. Utilizing TP53 as a mannequin, the ensuing primers supplied uniform protection and delicate variant detection at FFPE-derived DNA inputs >1ng.
) 2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21702 |
Bimake |
5 ml |
EUR 268.8 |
|
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
|
B21703 |
Bimake |
25 ml |
EUR 1027.2 |
|
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) 2x SYBR Green qPCR Master Mix (High ROX) |
|
B21402 |
Bimake |
5 ml |
EUR 268.8 |
|
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
|
B21403 |
Bimake |
25 ml |
EUR 1027.2 |
|
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
 AceQ qPCR SYBR® Green Master Mix |
|
Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
|
Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
 ChamQ SYBR qPCR Master Mix |
|
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix |
|
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
 ChamQ SYBR Color qPCR Master Mix |
|
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix |
|
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
 AceQ Universal SYBR qPCR Master Mix |
|
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
|
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
 ChamQ Universal SYBR qPCR Master Mix |
|
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
|
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
) ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
|
Q321-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
 Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 128.8 |
Taq Pro Universal SYBR qPCR Master Mix |
|
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 580 |
 miRNA Universal SYBR® qPCR Master Mix |
|
MQ101-01 |
Vazyme |
125 rxn(20 μl/rxn) |
EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
|
MQ101-02 |
Vazyme |
500 rxn(20 μl/rxn) |
EUR 290.4 |
) ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
|
Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
) ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
|
Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
) ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
|
Q421-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
) ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
|
Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
) ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
|
Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 Genious 2X SYBR Green Fast qPCR Mix |
|
RK21204 |
Abclonal |
1 mL |
Ask for price |
|
|
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17276-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
|
17277-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17278-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
|
17279-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17280-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
|
17281-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
 2X Universal SYBR Green Fast qPCR Mix |
|
RK21203 |
Abclonal |
1 mL |
Ask for price |
|
|
 TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
|
17270 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
|
17271 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
|
17272 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
|
17273 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
|
17274 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
|
17275 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
|
17276 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
|
17277 |
AAT Bioquest |
5 mL |
EUR 308 |
 lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|
M-PCR-173L |
MiTeGen |
960 reactions x 20 µl |
EUR 897 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
|
M-PCR-173S |
MiTeGen |
192 reactions x 20 µl |
EUR 224 |
|
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
 TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
|
17278 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
|
17279 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
|
17280 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
|
17281 |
AAT Bioquest |
5 mL |
EUR 308 |
) Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
|
RK21205 |
Abclonal |
1 ml |
Ask for price |
|
|
) amfiSure Ultra Fidelity PCR Master Mix(2X) |
|
P0346-001 |
GenDepot |
1ml |
EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
|
P0346-002 |
GenDepot |
2x1ml |
EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
|
P0346-005 |
GenDepot |
5x1ml |
EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
|
P0346-010 |
GenDepot |
10x1ml |
EUR 1632 |
 RT Master Mix for qPCR |
|
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
 AceQ qPCR Probe Master Mix |
|
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR Probe Master Mix |
|
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
) HotStart PCR Master mix (2X, Green Dye) |
|
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
|
HotStart PCR Master mix (2X, Green Dye) |
|
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
|
 Entrans 2X qPCR Probe Master Mix |
|
RK21208 |
Abclonal |
40 RXN |
Ask for price |
|
|
) Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
|
31020-2 |
Biotium |
50x1mL |
EUR 4291.2 |
|
Description: Minimum order quantity: 1 unit of 50x1mL |
) Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
|
31020-1 |
Biotium |
5x1mL |
EUR 524.4 |
|
Description: Minimum order quantity: 1 unit of 5x1mL |
) Green-2-Go qPCR Mastermix (500X20ul Rxn) |
|
QPCR004-S |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
|
) Fast EvaGreen Master Mix for qPCR (5000 rxn) |
|
31003-2 |
Biotium |
50x1ML |
EUR 3739.2 |
|
Description: Minimum order quantity: 1 unit of 50x1ML |
) Fast EvaGreen Master Mix for qPCR(200 rxn) |
|
31003 |
Biotium |
2x1ML |
EUR 244.8 |
|
Description: Minimum order quantity: 1 unit of 2x1ML |
) Fast EvaGreen Master Mix for qPCR (500 rxn) |
|
31003-1 |
Biotium |
5x1ML |
EUR 463.2 |
|
Description: Minimum order quantity: 1 unit of 5x1ML |
) Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
|
QPCR001-R |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
|
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
|
31041-20mL |
Biotium |
2x10mL |
EUR 592.8 |
|
Description: Minimum order quantity: 1 unit of 2x10mL |
) RT Master Mix for qPCR (gDNA digester plus) |
|
HY-K0511 |
MedChemExpress |
1 mL (100 rxns) |
EUR 378 |
) Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
|
QPCR003-iC |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
|
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
|
31041-1 |
Biotium |
5x1mL |
EUR 326.4 |
|
Description: Minimum order quantity: 1 unit of 5x1mL |
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
|
31041-T |
Biotium |
1mL |
EUR 136.8 |
|
Description: Minimum order quantity: 1 unit of 1mL |
) Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
|
QPCR006-NODYE |
Bio Basic |
4X1.25ml |
EUR 326.22 |
|
|
) Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
|
QPCR002-L |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
|
 TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
|
17282-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
|
17283-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
 TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17284-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17285-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
 TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
|
17286-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
|
17287-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
 TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
|
17282 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
|
17283 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17284 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17285 |
AAT Bioquest |
5 mL |
EUR 308 |
) Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
|
31020-T |
Biotium |
1mL |
EUR 160.8 |
|
Description: Minimum order quantity: 1 unit of 1mL |
 TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
|
17286 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
|
17287 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|
17288-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|
17289-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
) Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
|
QPCR005-NODYE |
Bio Basic |
4X1.25ml |
EUR 303.25 |
|
|
 TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17290-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17291-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
 TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
|
17292-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
|
17293-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
|
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
) Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
|
31014-1 |
Biotium |
5x1mL |
EUR 524.4 |
|
Description: Minimum order quantity: 1 unit of 5x1mL |
) Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
|
31014-2 |
Biotium |
50x1mL |
EUR 4291.2 |
|
Description: Minimum order quantity: 1 unit of 50x1mL |
) Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
|
31015-1 |
Biotium |
5x1mL |
EUR 524.4 |
|
Description: Minimum order quantity: 1 unit of 5x1mL |
) Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
|
31015-2 |
Biotium |
50x1mL |
EUR 4291.2 |
|
Description: Minimum order quantity: 1 unit of 50x1mL |
 TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|
17288 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|
17289 |
AAT Bioquest |
5 mL |
EUR 308 |
, packed in 1,25 ml vials) qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
|
S190 |
GeneOn |
100 rcs (2,5 ml) |
EUR 165.6 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
|
S190L |
GeneOn |
500 rcs (12,5 ml) |
EUR 627.6 |
 TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17290 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|
17291 |
AAT Bioquest |
5 mL |
EUR 308 |
 TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
|
17292 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
|
17293 |
AAT Bioquest |
5 mL |
EUR 308 |
) Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
|
31003-T |
Biotium |
1ML |
EUR 147.6 |
|
Description: Minimum order quantity: 1 unit of 1ML |
: (1mL)) Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
|
31014-T |
Biotium |
1mL |
EUR 160.8 |
|
Description: Minimum order quantity: 1 unit of 1mL |
: (1mL)) Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
|
31015-T |
Biotium |
1mL |
EUR 160.8 |
|
Description: Minimum order quantity: 1 unit of 1mL |
, packed in 1,25 ml vials) qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
|
S200 |
GeneOn |
100 rcs (2,5 ml) |
EUR 190.8 |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
|
S200L |
GeneOn |
500 rcs (12,5 ml) |
EUR 734.4 |
 (500 reactions)) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
|
9-31045 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
|
31042-1 |
Biotium |
5x1mL |
EUR 326.4 |
|
Description: Minimum order quantity: 1 unit of 5x1mL |
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
|
31042-20mL |
Biotium |
2x10mL |
EUR 592.8 |
|
Description: Minimum order quantity: 1 unit of 2x10mL |
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
|
31042-T |
Biotium |
1mL |
EUR 136.8 |
|
Description: Minimum order quantity: 1 unit of 1mL |
 (500 reactions)) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
|
9-31046 |
Biotium |
-
EUR 136.80
-
EUR 578.40
-
EUR 319.20
|
|
|
Description: Minimum order quantity: 1 unit of 5x1mL |
Future work will prolong the design pipeline to bigger and extra advanced panels, enabling fast creation of single-tube focused sequencing assays for quite a lot of functions.
