Survival of inoculated fungal strains in a brand new surroundings performs a important position in practical efficiency, however few research have centered on strain-specific quantitative PCR (qPCR) strategies for monitoring useful fungi.
On this research, the Trichoderma guizhouense pressure NJAU 4742 (remodeled with the gfp gene and named gfp-NJAU 4742), which reveals a growth-promoting impact via phytohormone manufacturing and pathogen antagonism, was chosen as a mannequin to design strain-specific primer pairs utilizing two steps of genomic sequence comparability to detect its abundance in soil.
After a second comparability with the carefully associated species T. harzianum CBS 226-95 to additional differentiate the strain-specific fragments that had proven no homology to any sequence deposited within the databases used within the first comparability, ten primer pairs had been designed from the entire genome.
In the meantime, three primer pairs, P11, P12 and P13, had been additionally designed from the inserted fragment containing the gfp gene. After verification testing with three varieties of subject soils, primer pairs P6, P7 and P8 had been additional chosen by comparability with P11, P12 and P13.
A sensible check utilizing a pot experiment confirmed that steady colonization of gfp-NJAU 4742 in pepper rhizosphere soil could possibly be detected utilizing primer pairs P6 and P7, displaying no important distinction from the outcomes of primers P11 and P12.
Therefore, the technique described right here for designing fungal-strain-specific primers might theoretically be used for some other fungi for which the entire genome sequence is on the market in a database, and the qPCR methodology developed will also be used to additional monitor the inhabitants dynamics of various strains primarily based on the designed primers.
Quantitative PCR primer design impacts quantification of dsRNA-mediated gene knockdown.
RNA interference (RNAi) is a robust software for learning features of candidate genes in each mannequin and nonmodel organisms and a promising approach for therapeutic functions. Profitable software of this system depends on the accuracy and reliability of strategies used to quantify gene knockdown.
With the limitation within the availability of antibodies for detecting proteins, quantitative PCR (qPCR) stays the popular technique for quantifying goal gene knockdown after dsRNA therapy. We evaluated how qPCR primer binding web site and goal gene expression ranges have an effect on quantification of intact mRNA transcripts following dsRNA-mediated RNAi.

The usage of primer pairs focusing on the mRNA sequence inside the dsRNA goal area did not reveal a major lower in goal mRNA transcripts for genes with low expression ranges, however not for a extremely expressed gene. Against this, important knockdown was detected in all instances with primer pairs focusing on the mRNA sequence extending past the dsRNA goal area, whatever the expression ranges of the goal gene.
Our outcomes counsel that at the least for genes with low expression ranges, quantifying the effectivity of dsRNA-mediated RNAi with primers amplifying sequences fully contained within the dsRNA goal area ought to be prevented because of the danger of false-negative outcomes. As an alternative, primer pairs extending past the dsRNA goal area of the mRNA transcript sequences ought to be used for correct and dependable quantification of silencing effectivity.
Combining bioinformatics and standard PCR optimization technique for one-time design of high-specificity primers for WRKY gene household utilizing unigene database.
Gene households, just like the conserved transcription issue households, evolve via gene duplications and share reasonable similarity between member genes. Lack of genomic information makes it tough to design high-specificity primers to the goal genes.
Moreover, many primers under-perform in extremely delicate assays like quantitative PCR as a result of problems with thermodynamic nature, thereby growing the fee and time for evaluation. A strategy involving intra-species and inter-generic bioinformatic sequence comparability mixed with thermodynamic estimation of primer efficiency was used for one-time design of gene particular primers for various WRKYs, Mitogen Activated Protein-kinases and N-methyltransferases of Coffea canephora with out the help of genome sequence assets.
Out of a complete 37 primer units together with 31 pairs of primers for WRKY from 34 mined WRKY Unigenes/ESTs and 6 pairs for genes coding for MAP kinases and NBS-LRR proteins, 32 units exhibited excessive specificity of amplification upon genome evaluation in addition to within the high-resolution soften evaluation.
Moreover, PCR optimization strategies-both in silico and experimental-indicated a superior efficiency of the primer units for various functions like quantitative PCR and speedy amplification of cDNA ends. Just one set of primer resulted in mis-priming upon affirmation by DNA sequencing of the cloned amplicons.
The intra-species variations and inter-generic similarities guarantee excessive specificity of primers in all instances studied. The process allowed design of primers for the use in numerous downstream functions with excessive efficiency, specificity, yield and ease-of-use.
Primer design and amplification efficiencies are essential for reliability of quantitative PCR research of caffeine biosynthetic N-methyltransferases in espresso.
Primers having suboptimal amplification efficiencies had been proven to falsely characterize fold change expression of the N-methyltransferases gene household concerned in caffeine biosynthesis in Coffea canephora.
To check this phenomenon, the position of stability of the interior reference gene, in addition to the amplification effectivity correction of the primers was investigated. GAPDH and Ubiquitin exhibited a great stability for learning the ontogeny of endosperm tissue, in addition to the leaf transcriptome throughout stress from salicylic acid, methyl jasmonate, PEG-mediated drought and sudden publicity to mild.
Ubiquitin manifested low variation in Cq beneath all these stress regimes and in endosperm ontogeny with 30.1-30.9 in one of the best dataset and 28.8-30.9 in probably the most deviating dataset. It was noticed that issues arising as a result of improper amplification effectivity of the goal or reference genes or each may result in misinterpretation of gene expression ranges.
Quantitative RT-PCR carried out at a sub-optimal effectivity of GAPDH reference gene at 1.68 led to the defective interpretation of two.007 folds upregulation by the 2-ΔΔCt technique and 1.705 folds upregulation by Effectivity technique for the primary NMT (Xanthosine methyltransferase), which truly is repressed throughout darkish acclimatization of espresso vegetation.
Effectivity correction improved the reliability of the expression information and likewise indicated a downregulation of this gene by 0.485 folds and 0.474 folds utilizing 2-ΔΔCt and E technique, respectively, in concordance to earlier studies.
The egg drop syndrome (76) PCR kit |
PCR-V044-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of The egg drop syndrome (76) |
The egg drop syndrome (76) PCR kit |
PCR-V044-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of The egg drop syndrome (76) |
PCR Mix |
L5051100 |
Biochain |
2.5 ml |
EUR 107 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
2×Taq PCR Master Mix(with dye) |
K1034-1 |
ApexBio |
1 ml |
EUR 73 |
Egg Drop Syndrome RT PCR kit |
RTq-V026-100D |
Bioingentech |
100T |
EUR 628.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: A Real-Time PCR kit for detection of Egg Drop Syndrome . |
Egg Drop Syndrome RT PCR kit |
RTq-V026-150D |
Bioingentech |
150T |
EUR 701 |
- Contact us in order to know the reactivity of the kit.
|
Description: A Real-Time PCR kit for detection of Egg Drop Syndrome . |
Egg Drop Syndrome RT PCR kit |
RTq-V026-50D |
Bioingentech |
50T |
EUR 532.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: A Real-Time PCR kit for detection of Egg Drop Syndrome . |
Individual Reaction Mix 1 |
G065-1 |
ABM |
200 reactions |
EUR 167 |
PCR-EZ D-PCR MASTER MIX |
BS294 |
BBI Biotech |
100RXN, 100prep |
EUR 93.5 |
- Product category: PCR Related/PCR Premix (dPCR)/dPCR Premix + Taq
|
amfiSure PCR Master Mix |
P0311-010 |
GenDepot |
2x50 rxns |
EUR 126 |
amfiSure PCR Master Mix |
P0311-025 |
GenDepot |
5X50 rxns |
EUR 165 |
amfiSure PCR Master Mix |
P0311-050 |
GenDepot |
10X50 rxns |
EUR 219 |
amfiSure PCR Master Mix |
P0311-100 |
GenDepot |
10x1ml |
EUR 286 |
amfiSure PCR Master Mix |
P0311-125 |
GenDepot |
15X50 rxns |
EUR 278 |
amfiSure PCR Master Mix |
P0311-200 |
GenDepot |
20x1ml |
EUR 469 |
amfiSure PCR Master Mix |
P0311-250 |
GenDepot |
50x50 rxns |
EUR 752 |
amfiSure PCR Master Mix |
P0311-500 |
GenDepot |
100x50 rxns |
EUR 1258 |
amfiXpand PCR Master Mix |
P0331-010 |
GenDepot |
2X50 rxns |
EUR 151 |
amfiXpand PCR Master Mix |
P0331-025 |
GenDepot |
5X50 rxns |
EUR 273 |
amfiXpand PCR Master Mix |
P0331-050 |
GenDepot |
10X50 rxns |
EUR 448 |
amfiXpand PCR Master Mix |
P0331-250 |
GenDepot |
50x50 rxns |
EUR 1873 |
Ready? PCR Mix-Dye |
M1128-1000 |
Biovision |
|
EUR 512 |
Ready? PCR Mix-Dye |
M1128-200 |
Biovision |
|
EUR 191 |
Image Ready? PCR Mix |
M1129-200 |
Biovision |
|
EUR 294 |
Robust Ready? PCR Mix |
M1130-1000 |
Biovision |
|
EUR 615 |
Robust Ready? PCR Mix |
M1130-200 |
Biovision |
|
EUR 213 |
Rigor? PCR Mix-Dye |
M1133-200 |
Biovision |
|
EUR 321 |
Breeze? PCR Mix-Dye |
M1135-200 |
Biovision |
|
EUR 267 |
Distant? PCR Mix-Dye |
M1137-200 |
Biovision |
|
EUR 294 |
Image Distant? PCR Mix |
M1138-200 |
Biovision |
|
EUR 321 |
Advance? PCR Mix-Dye |
M1140-200 |
Biovision |
|
EUR 376 |
Fire Start? PCR Mix |
M1141-200 |
Biovision |
|
EUR 376 |
Whole Blood PCR Mix |
M1143-200 |
Biovision |
|
EUR 321 |
Egg Drop Syndrome One-Step PCR kit |
Oneq-V026-100D |
Bioingentech |
100T |
EUR 747.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Egg Drop Syndrome. |
Egg Drop Syndrome One-Step PCR kit |
Oneq-V026-150D |
Bioingentech |
150T |
EUR 838.75 |
- Contact us in order to know the reactivity of the kit.
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Egg Drop Syndrome. |
Egg Drop Syndrome One-Step PCR kit |
Oneq-V026-50D |
Bioingentech |
50T |
EUR 628.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Egg Drop Syndrome. |
2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn |
W0655-1 |
101Bio |
NULL |
EUR 0 |
MyTaq Blood PCR Mix, 2x |
BIO-25053/S |
Bioline |
Sample |
Ask for price |
MyTaq Blood PCR Mix, 2x |
BIO-25054 |
Bioline |
250 Reactions |
Ask for price |
amfiSure Prime PCR Master Mix |
P1311-025 |
GenDepot |
5X50 rxns |
EUR 161 |
amfiSure Prime PCR Master Mix |
P1311-050 |
GenDepot |
10X50 rxns |
EUR 219 |
amfiSure Prime PCR Master Mix |
P1311-125 |
GenDepot |
15X50 rxns |
EUR 273 |
amfiSure Prime PCR Master Mix |
P1311-250 |
GenDepot |
50x50 rxns |
EUR 739 |
amfiSure Prime PCR Master Mix |
P1311-500 |
GenDepot |
100x50 rxns |
EUR 1238 |
amfiSure Advanced PCR Master Mix |
P2311-025 |
GenDepot |
5X50 rxns |
EUR 165 |
amfiSure Advanced PCR Master Mix |
P2311-050 |
GenDepot |
10X50 rxns |
EUR 223 |
amfiSure Advanced PCR Master Mix |
P2311-125 |
GenDepot |
15X50 rxns |
EUR 282 |
amfiSure Advanced PCR Master Mix |
P2311-250 |
GenDepot |
50x50 rxns |
EUR 752 |
amfiSure Advanced PCR Master Mix |
P2311-500 |
GenDepot |
100x50 rxns |
EUR 1279 |
Robust Ready? PCR Mix-Dye |
M1131-1000 |
Biovision |
|
EUR 615 |
Robust Ready? PCR Mix-Dye |
M1131-200 |
Biovision |
|
EUR 213 |
Fire Start? PCR Mix-Dye |
M1142-200 |
Biovision |
|
EUR 376 |
mRNAExpress Tail PCR mix (5uM) |
MR-TAIL-PR |
SBI |
100 ul |
EUR 164 |
|
The egg drop syndrome (76) RT PCR kit |
RTq-V044-100D |
Bioingentech |
100T |
EUR 628.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: A Real-Time PCR kit for detection of The egg drop syndrome (76) . |
The egg drop syndrome (76) RT PCR kit |
RTq-V044-150D |
Bioingentech |
150T |
EUR 701 |
- Contact us in order to know the reactivity of the kit.
|
Description: A Real-Time PCR kit for detection of The egg drop syndrome (76) . |
The egg drop syndrome (76) RT PCR kit |
RTq-V044-50D |
Bioingentech |
50T |
EUR 532.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: A Real-Time PCR kit for detection of The egg drop syndrome (76) . |
PRICE DROP |
110-012 |
GeneOn |
4 x 1000 µl |
EUR 133 |
PRICE DROP |
110-012L |
GeneOn |
5x 4 x 1000 µl |
EUR 518 |
PRICE DROP |
110-012XL |
GeneOn |
10x 4 x 1000 µl |
EUR 939 |
The egg drop syndrome (76) One-Step PCR kit |
Oneq-V044-100D |
Bioingentech |
100T |
EUR 747.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of The egg drop syndrome (76). |
The egg drop syndrome (76) One-Step PCR kit |
Oneq-V044-150D |
Bioingentech |
150T |
EUR 838.75 |
- Contact us in order to know the reactivity of the kit.
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of The egg drop syndrome (76). |
The egg drop syndrome (76) One-Step PCR kit |
Oneq-V044-50D |
Bioingentech |
50T |
EUR 628.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of The egg drop syndrome (76). |
Taq 5x PCR Mix 12.5 mM |
BT10402 |
Bioatlas |
250rxn |
EUR 109 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq 2x PCR Mix 100 rxn |
BA01501 |
Bioatlas |
100rxn |
EUR 94 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
HotTaq 2x PCR Mix 100 rxn |
BA01503 |
Bioatlas |
100rxn |
EUR 120 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
RedTaq 2x PCR Mix 100 rxn |
BA01507 |
Bioatlas |
100rxn |
EUR 146 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
amfiSure ONE PCR Master Mix(2X) |
P7000-005 |
GenDepot |
5x1 ml |
EUR 219 |
amfiSure ONE PCR Master Mix(2X) |
P7000-010 |
GenDepot |
10x1 ml |
EUR 317 |
amfiSure ONE PCR Master Mix(2X) |
P7000-050 |
GenDepot |
50x1 ml |
EUR 1211 |
amfiSure ONE PCR Master Mix(2X) |
P7000-100 |
GenDepot |
100x1 ml |
EUR 2102 |
AXYPREP MAG PCR NORMALIZER KIT- 1 ML - (SAMPLE SIZE) |
MAG-PCR-NM-1 |
CORNING |
1/pk |
EUR 73 |
Description: Bioscience Mag Beads; Magnetic PCR Normaliser |
AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE) |
MAG-PCR-CL-1 |
CORNING |
1/pk |
EUR 58 |
Description: Bioscience Mag Beads; Magnetic PCR Clean Up |
Poliovirus 1 (PV1) PCR kit |
PCR-H659-48R |
Bioingentech |
50T |
EUR 524.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Poliovirus 1 (PV1) |
Poliovirus 1 (PV1) PCR kit |
PCR-H659-96R |
Bioingentech |
100T |
EUR 669.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Poliovirus 1 (PV1) |
Psittacid herpesvirus 1 PCR kit |
PCR-V014-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Psittacid herpesvirus 1 |
Psittacid herpesvirus 1 PCR kit |
PCR-V014-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Psittacid herpesvirus 1 |
Equine herpesvirus 1 PCR kit |
PCR-V144-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Equine herpesvirus 1 |
Equine herpesvirus 1 PCR kit |
PCR-V144-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Equine herpesvirus 1 |
Bovine herpesvirus 1 PCR kit |
PCR-V182-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Bovine herpesvirus 1 |
Bovine herpesvirus 1 PCR kit |
PCR-V182-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Bovine herpesvirus 1 |
Bluetongue virus 1 PCR kit |
PCR-V226-48R |
Bioingentech |
50T |
EUR 524.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Bluetongue virus 1 |
Bluetongue virus 1 PCR kit |
PCR-V226-96R |
Bioingentech |
100T |
EUR 669.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Bluetongue virus 1 |
Smart 2X PCR pre-mix Pfu (30ul) |
9K-002-0038 |
BBI Biotech |
30uL, 30uL |
EUR 581.14 |
- Product category: PCR Related/PCR Premix/PCR Premix + Pfu
|
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
BBI Biotech |
5X1mL, 5ml |
EUR 209.5 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
BBI Biotech |
1ml |
EUR 96.4 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9295 |
BBI Biotech |
1ml |
EUR 84.8 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9296 |
BBI Biotech |
5ml |
EUR 189.2 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
Taq PCR Master Mix (2X, Red Dye) |
BS9297 |
BBI Biotech |
1ml |
EUR 84.8 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
Taq PCR Master Mix (2X, Red Dye) |
BS9298 |
BBI Biotech |
5ml |
EUR 189.2 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
2×Taq PCR Master Mix(with dye) |
K1034-100 |
ApexBio |
100×1 ml |
EUR 792 |
2×Taq PCR Master Mix(with dye) |
K1034-20 |
ApexBio |
20×1 ml |
EUR 282 |
2×Taq PCR Master Mix(with dye) |
K1034-5 |
ApexBio |
5×1 ml |
EUR 119 |
2×Taq PCR Master Mix(with dye) |
K1034-50 |
ApexBio |
50×1 ml |
EUR 514 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-010 |
GenDepot |
2x50 rxns |
EUR 253 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-025 |
GenDepot |
5X50 rxns |
EUR 455 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-050 |
GenDepot |
10X50 rxns |
EUR 779 |
amfiFusion High Fidelity PCR Master Mix(2X) |
P0342-100 |
GenDepot |
20x50 rxns |
EUR 1333 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-001 |
GenDepot |
1ml |
EUR 280 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-002 |
GenDepot |
2x1ml |
EUR 482 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-005 |
GenDepot |
5x1ml |
EUR 828 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
P0346-010 |
GenDepot |
10x1ml |
EUR 1360 |
Reverse Transcriptase RT PCR Master Mix (5X) |
MB1002-100reactions |
BosterBio |
100 reactions |
EUR 514 |
Reverse Transcriptase RT PCR Master Mix (5X) |
MB1002-200reactions |
BosterBio |
200 reactions |
EUR 746 |
Reverse Transcriptase RT PCR Master Mix (5X) |
MB1002-25reactions |
BosterBio |
25 reactions |
EUR 189 |
MULTIPLEX KIT PCR MASTITIS PCR kit |
PCR-MPX218-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
MULTIPLEX KIT PCR MASTITIS PCR kit |
PCR-MPX218-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
Herpes simplex type 1 PCR kit |
PCR-H426-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Herpes simplex type 1 |
Herpes simplex type 1 PCR kit |
PCR-H426-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Herpes simplex type 1 |
Dengue virus type 1 PCR kit |
PCR-H521-48R |
Bioingentech |
50T |
EUR 524.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Dengue virus type 1 |
Dengue virus type 1 PCR kit |
PCR-H521-96R |
Bioingentech |
100T |
EUR 669.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Dengue virus type 1 |
Canine adenovirus type 1 PCR kit |
PCR-V077-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Canine adenovirus type 1 |
Canine adenovirus type 1 PCR kit |
PCR-V077-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Canine adenovirus type 1 |
Caprine herpes virus 1 PCR kit |
PCR-V312-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Caprine herpes virus 1 |
Caprine herpes virus 1 PCR kit |
PCR-V312-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Caprine herpes virus 1 |
Mouse parvovirus type 1 PCR kit |
PCR-V324-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Mouse parvovirus type 1 |
Mouse parvovirus type 1 PCR kit |
PCR-V324-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Mouse parvovirus type 1 |
Borna disease viruses 1 PCR kit |
PCR-V605-48R |
Bioingentech |
50T |
EUR 524.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Borna disease viruses 1 |
Borna disease viruses 1 PCR kit |
PCR-V605-96R |
Bioingentech |
100T |
EUR 669.4 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of Borna disease viruses 1 |
Fast Probe Master Mix (500 rxn) |
31005-1 |
Biotium |
5x1ML |
EUR 466 |
Description: Minimum order quantity: 1 unit of 5x1ML |
2xEs Taq Master Mix (with Dyes) |
W0690-1 |
101Bio |
NULL |
EUR 0 |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
PCR-MPX401-48D |
Bioingentech |
50T |
EUR 425.8 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
PCR-MPX401-96D |
Bioingentech |
100T |
EUR 521.5 |
- Contact us in order to know the reactivity of the kit.
|
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria |
Primer Mix for Junction PCR to Confirm AAVS1 Integration Site (compatible with GE620A-1, GE622A-1, GE624A-1 and CAS620A-1), 50ul at 5uM for each primer set |
GE640PR-1 |
SBI |
50 reactions |
EUR 159 |
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Drop Delay Calibration Particles |
DDCP-70-2 |
Spherotech |
2 mL |
EUR 283 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
Drop Delay Calibration Particles |
DDCP-70-20 |
Spherotech |
20 mL |
EUR 1918 |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
Human T-lymphotropic Virus 1 PCR kit |
PCR-H657-48R |
Bioingentech |
50T |
EUR 524.4 |
- Contact us in order to know the reactivity of the kit.
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Description: An conventional PCR kit for detection of Human T-lymphotropic Virus 1 |
Human T-lymphotropic Virus 1 PCR kit |
PCR-H657-96R |
Bioingentech |
100T |
EUR 669.4 |
- Contact us in order to know the reactivity of the kit.
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Description: An conventional PCR kit for detection of Human T-lymphotropic Virus 1 |
Equine rotavirus strain H-1 PCR kit |
PCR-V161-48R |
Bioingentech |
50T |
EUR 524.4 |
- Contact us in order to know the reactivity of the kit.
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Description: An conventional PCR kit for detection of Equine rotavirus strain H-1 |
Equine rotavirus strain H-1 PCR kit |
PCR-V161-96R |
Bioingentech |
100T |
EUR 669.4 |
- Contact us in order to know the reactivity of the kit.
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Description: An conventional PCR kit for detection of Equine rotavirus strain H-1 |
Therefore, effectivity correction of the primers having suboptimal efficiencies is an absolute prerequisite for the correct calculation of fold change utilizing quantitative RT-PCR.